Quantitation of polysorbate 20 in protein solutions using mixed-mode chromatography and evaporative light scattering detection

Journal of Chromatography A

Volumn 1215, Issue 1-2, 2008, Pages 156-160

  Hewitt, Daniel ^a   Zhang, Taylor ^a   Kao, Yung Hsiang ^a  

^a GENENTECH INC   (United States)

Author keywords

Evaporative light scattering detection; Mixed mode chromatography; Polysorbate; Protein; Sample preparation

Indexed keywords

AMINES; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; LIGHT SCATTERING; ORGANIC ACIDS; REFRACTION; RESINS; SCATTERING; SORPTION; SURFACE ACTIVE AGENTS;

ELECTROSTATIC REPULSIONS; EVAPORATIVE LIGHT SCATTERING DETECTION; EVAPORATIVE LIGHT SCATTERINGS; HARMONISATION; IONIC SURFACTANTS; MIXED-MODE CHROMATOGRAPHY; NO COMPLEXES; POLYSORBATE; POLYSORBATE 20; PROTEIN SAMPLES; PROTEIN SOLUTIONS; QUATERNARY AMINES; SAMPLE PREPARATION; SINGLE PEAKS; STATIONARY PHASE; TRITON X-100; VOID VOLUMES;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

AMINE; POLYSORBATE 20; RESIN;

ACCURACY; ARTICLE; CHEMICAL STRUCTURE; CHROMATOGRAPHY; HYDROPHILICITY; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; LIGHT; POLYSORBATES; PROTEINS; REPRODUCIBILITY OF RESULTS; SCATTERING, RADIATION; SENSITIVITY AND SPECIFICITY; SOLUTIONS;

EID: 57049117318     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2008.11.017     Document Type: Article

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