Down-regulation of flavonoid 3′-hydroxylase gene expression by virus-induced gene silencing in soybean reveals the presence of a threshold mRNA level associated with pigmentation in pubescence

Journal of Plant Physiology

Volumn 166, Issue 1, 2009, Pages 32-39

  Nagamatsu, Atsushi ^a   Masuta, Chikara ^a   Matsuura, Hideyuki ^a   Kitamura, Keisuke ^a   Abe, Jun ^a   Kanazawa, Akira ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Cucumber mosaic virus; Flavonoid 3 hydroxylase; Pigmentation; Soybean (Glycine max); Virus induced gene silencing

Indexed keywords

CYTOCHROME P450; FLAVONOID 3' HYDROXYLASE; FLAVONOID 3'-HYDROXYLASE; KAEMPFEROL; KAEMPFEROL DERIVATIVE; MESSENGER RNA; QUERCETIN; SMALL INTERFERING RNA;

ARTICLE; DOWN REGULATION; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; METABOLISM; PIGMENTATION; PLANT SEED; PLANT VIRUS; SOYBEAN; VIROLOGY;

CYTOCHROME P-450 ENZYME SYSTEM; DOWN-REGULATION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, PLANT; GENE SILENCING; KAEMPFEROLS; PIGMENTATION; PLANT VIRUSES; QUERCETIN; RNA, MESSENGER; RNA, SMALL INTERFERING; SEEDS; SOYBEANS;

CUCUMBER MOSAIC VIRUS; GLYCINE MAX;

EID: 56949100823     PISSN: 01761617     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jplph.2008.02.002     Document Type: Article

References (26)

1. Burch-Smith T.M., Anderson J.C., Martin G.B., and Dinesh-Kumar S.P. Applications and advantages of virus-induced gene silencing for gene function studies in plants. Plant J 39 (2004) 734-746
2. Buttery B.R., and Buzzell R.I. Varietal differences in leaf flavonoids of soybeans. Crop Sci 13 (1973) 103-106
3. Cone K.C., Cocciolone S.M., Moehlenkamp C.A., Weber T., Drummond B.J., Tagliani L.A., et al. Role of the regulatory gene pl in the photocontrol of maize anthocyanin pigmentation. Plant Cell 5 (1993) 1807-1816
4. Goto K., Kanazawa A., Kusaba M., and Masuta C. A simple and rapid method to detect plant siRNAs using nonradioactive probes. Plant Mol Biol Rep 21 (2003) 51-58
5. Goto K., Kobori T., Kosaka Y., Natsuaki T., and Masuta C. Characterization of silencing suppressor 2b of cucumber mosaic virus based on examination of its small RNA-binding abilities. Plant Cell Physiol 48 (2007) 1050-1060
6. Hamilton A.J., and Baulcombe D.C. A species of small antisence RNA in posttranscriptional gene silencing in plants. Science 286 (1999) 950-952
7. Iwashina T., Benitez E.R., and Takahashi R. Analysis of flavonoids in pubescence of soybean near-isogenic lines for pubescence color loci. J Hered 97 (2006) 438-443
8. Koes R., Verweij W., and Quattrocchio F. Flavonoids: a colorful model for the regulation and evolution of biochemical pathways. Trends Plant Sci 10 (2005) 236-242
9. Koseki M., Goto K., Masuta C., and Kanazawa A. The star-type color pattern in Petunia hybrida 'Red Star' flowers is induced by sequence-specific degradation of chalcone synthase RNA. Plant Cell Physiol 46 (2005) 1879-1883
10. Maquat L.E. Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics. Nat Rev Mol Cell Biol 5 (2004) 89-99
11. Masuta C., Tanaka H., Uehara K., Kuwata S., Koiwai A., and Noma M. Broad resistance to plant viruses in transgenic plants conferred by antisense inhibition of a host gene essential in S-adenosylmethionine-dependent transmethylation reactions. Proc Natl Acad Sci USA 92 (1995) 6117-6121
12. Metzlaff M., O'Dell M., Cluster P.D., and Fravell R.B. RNA-mediated degradation and chalcone synthase A silencing in petunia. Cell 88 (1997) 845-854
13. Nagamatsu A., Masuta C., Senda M., Matsuura H., Kasai A., Hong J.S., et al. Functional analysis of soybean genes involved in flavonoid biosynthesis by virus-induced gene silencing. Plant Biotechnol J 5 (2007) 778-790
14. Napoli C., Lemieux C., and Jorgensen R. Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2 (1990) 279-289
15. Otagaki S., Arai M., Takahashi A., Goto K., Hong J.S., Masuta C., et al. Rapid induction of transcriptional and post-transcriptional gene silencing using a novel Cucumber mosaic virus vector. Plant Biotechnol 23 (2006) 259-265
16. Palmer R.G., Pfeiffer T.W., Buss G.R., and Kilen T.C. Qualitative genetics. In: Boerma H.R., and Specht J.E. (Eds). Soybeans: improvement, production, and uses. third ed (2004), American Society of Agronomy Inc., Crop Science Society of America Inc., Soil Science Society of America Inc., Madison 137-233
17. Porter P.M., Banwart W.L., and Hassett J.J. Phenolic acids and flavonoids in soybean root and leaf extracts. Environ Exp Bot 26 (1986) 65-73
18. Romani A., Vignolini P., Galardi C., Aroldi C., Vazzana C., and Heimler D. Polyphenolic content in different plant parts of soy cultivars grown under natural conditions. J Agric Food Chem 51 (2003) 5301-5306
19. Ross J.A., and Kasum C.M. Dietary flavonoids: bioavailability, metabolic effects, and safety. Annu Rev Nutr 22 (2002) 19-34
20. Schoenbohm C., Martins S., Eder C., Forkmann G., and Weisshaar B. Identification of the Arabidopsis thaliana flavonoid 3′-hydroxylase gene and functional expression of the encoded P450 enzyme. Biol Chem 381 (2000) 749-753
21. Senda M., Masuta C., Ohnishi S., Goto K., Kasai A., Sano T., et al. Pattering of virus-infected Glycine max seed coat is associated with suppression of endogenous silencing of chalcone synthase genes. Plant Cell 16 (2004) 807-818
22. Takahashi R., and Asanuma S. Association of T gene with chilling tolerance in soybean. Crop Sci 36 (1996) 559-562
23. Toda K., Yang D., Yamanaka N., Watanabe S., Harada K., and Takahashi R. A single-base deletion in soybean flavonoid 3′-hydroxylase gene is associated with gray pubescence color. Plant Mol Biol 50 (2002) 187-196
24. Treutter D. Significance of flavonoids in plant resistance and enhancement of their biosynthesis. Plant Biol 7 (2005) 581-591
25. Yu O., Shi J., Hession A.O., Maxwell C.A., McGonigle B., and Odell J.T. Metabolic engineering to increase isoflavone biosynthesis in soybean seed. Phytochemistry 63 (2003) 753-763
26. Zabala G., and Vodkin L. Cloning of the pleiotropic T locus in soybean and two recessive alleles that differentially affect structure and expression of the encoded flavonoid 3′ hydroxylase. Genetics 163 (2003) 295-309