Heterologous expression and biochemical characterization of an α1,2-mannosidase encoded by the Candida albicans MNS1 gene

Memorias do Instituto Oswaldo Cruz

Volumn 103, Issue 7, 2008, Pages 724-730

  Mora Montes, Héctor M ^a,c   López Romero, Everardo ^a   Zinker, Samuel ^b   Ponce Noyola, Patricia ^a   Flores Carreón, Arturo ^a  

^a UNIVERSITY OF GUANAJUATO   (Mexico)

^b DEPARTAMENTO DE FÍSICA   (Mexico)

^c UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

Candida albicans; Glycosyl hydrolase family 47; Heterologous expression; N glycosylation; Polyclonal antibodies; 1,2 mannosidase

Indexed keywords

CANDIDA ALBICANS; ESCHERICHIA COLI;

ANTIBODY; MANNOSIDASE; MANNOSYL OLIGOSACCHARIDE 1,2 ALPHA MANNOSIDASE; MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE;

ARTICLE; CANDIDA ALBICANS; ENZYME SPECIFICITY; ENZYMOLOGY; FUNGAL GENE; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING;

ANTIBODIES; CANDIDA ALBICANS; CLONING, MOLECULAR; GENES, FUNGAL; MANNOSIDASES; SUBSTRATE SPECIFICITY;

EID: 56749153888     PISSN: 00740276     EISSN: 16788060     Source Type: Journal    
DOI: 10.1590/S0074-02762008000700016     Document Type: Article

References (23)

1. Akao T, Yamaguchi M, Yahara A, Yoshiuchi K, Fujita H, Yamada O, Akita O, Ohmachi T, Asada YT, Yoshida T 2006. Cloning and expression of 1,2-α-mannosidase gene (fmanIB) from filamentous fungus Aspergillus oryzae: in vivo visualization of the FmanIBp-GFP fusion protein. Biosci Biotechnol Biochem 70: 471-479.
2. Bradford MM 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
3. Camirand A, Heysen A, Grondin B, Herscovics A 1991. Glycoprotein biosynthesis in Saccharomyces cerevisiae. Isolation and characterization of the gene encoding a specific processing α-mannosidase. J Biol Chem 266: 15120-15127.
4. Goers J 1993. Immunochemical techniques laboratory manual, Academic Press, San Diego, p. 15-18, 119-135.
5. Helenius A, Aebi M 2004. Roles of N-linked glycans in the endoplasmic reticulum. Annu Rev Biochem 73: 1019-1049.
6. Herscovics A 1999a. Importance of glycosidases in mammalian glycoprotein biosynthesis. Biochim Biophys Acta 1473: 96-107.
7. Herscovics A 1999b. Processing glycosidases of Saccharomyces cerevisiae. Biochim Biophys Acta 1426: 275-285.
8. Herscovics A 2001. Structure and function of Class I α1,2- mannosidases involved in glycoprotein synthesis and endoplasmic reticulum quality control. Biochimie 83: 757-762.
9. Herscovics A, Romero PA, Tremblay LO 2002. The specificity of the yeast and human class I ER α1,2-mannosidases involved in ER quality control is not as strict previously reported. Glycobiology 12: 14G-15G.
10. Ichishima I, Taya N, Ikeguchi M, Chiba Y, Nakamura M, Kawabata C, Inoue T, Takahashi K, Minetoki T, Ozeki K, Kumagai C, Gomi K, Yoshida T, Nakajima T 1999. Molecular and enzymic properties of recombinant 1,2-α-mannosidase from Aspergillus saitoi overexpressed in Aspergillus oryzae cells. Biochem J 339: 589-597.
11. Kornfeld R, Kornfeld S 1985. Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem 54: 631-664.
12. Mora-Montes HM, Bates S, Netea MG, Diaz-Jiménez DF, López-Romero E, Zinker S, Ponce-Noyola P, Kullberg BJ, Brown AJ, Odds FC, Flores-Carreón A, Gow NA 2007. Endoplasmic reticulum α-glycosidases of Candida albicans are required for N-glycosylation, cell wall integrity, and normal host-fungus interaction. Eukaryot Cell 6: 2184-2193.
13. 2 oligosaccharides by a purified α-mannosidase from Candida albicans. Glycobiology 14: 593-598.
14. Mora-Montes HM, López-Romero E, Zinker S, Ponce-Noyola P, Flores-Carreón A 2006. Purification of soluble α1,2-mannosidase from Candida albicans CAI-4. FEMS Microbiol Lett 256: 50-56.
15. Mora-Montes HM, Lopez-Romero E, Zinker S, Ponce-Noyola P, Flores-Carreón A 2008. Conversion of α1,2-mannosidase E-I from Candida albicans to α1,2-mannosidase E-II by limited proteolysis. Antonie Van Leeuwenhoek 93: 61-69.
16. Movsichoff F, Castro OA, Parodi AJ 2005. Characterization of Schizosaccharomyces pombe ER α-mannosidase: a reevaluation of the role of the enzyme on ER-associated degradation. Mol Biol Cell 16: 4714-4724.
17. Towbin H, Staehelin T, Gordon J 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
18. 2 isomer B during N-glycan biosynthesis. Glycobiology 9: 1073-1078.
19. Tremblay LO, Herscovics A 2000. Characterization of a cDNA encoding a novel human Golgi α1,2-mannosidase (IC) involved in N-glycans biosynthesis. J Biol Chem 275: 31655-31660.
20. Trombetta ES 2003. The contribution of N-glycans and their processing in the endoplasmic reticulum to glycoprotein biosynthesis. Glycobiology 13: 77R-91R.
21. Vázquez-Reyna AB, Balcázar-Orozco R, Flores-Carreón A 1993. Biosynthesis of glycoproteins in Candida albicans: biochemical characterization of a soluble α-mannosidase. FEMS Microbiol Lett 106: 321-325.
22. Vázquez-Reyna AB, Ponce-Noyola P, Calvo-Méndez C, López-Romero E, Flores-Carreón A 1999. Purification and biochemical characterization of two soluble α-mannosidases from Candida albicans. Glycobiology 9: 533-537.
23. 9 processing α-mannosidase. Glycobiology 1: 605-614.