High-performance liquid chromatographic method to measure protein l-isoaspartyl/d-aspartyl o-methyltransferase activity in cell lysates

Analytical Biochemistry

Volumn 384, Issue 2, 2009, Pages 207-212

  Furuchi, Takemitsu ^a   Kosugi, Sakurako ^a   Ohno, Keiko ^a   Egawa, Tsukasa ^a   Sekine, Masae ^a   Katane, Masumi ^a   Homma, Hiroshi ^a  

^a KITASATO UNIVERSITY   (Japan)

Author keywords

Enzyme activity; Fluorometric HPLC assay; Protein l isoaspartyl d aspartyl methyltransferase (PIMT)

Indexed keywords

CELLS; CHROMATOGRAPHIC ANALYSIS; CYTOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PEPTIDES;

BIOLOGICAL SAMPLES; FLUOROMETRIC HPLC; HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHODS; LIMITED SENSITIVITY; METHYLTRANSFERASES; O-METHYLTRANSFERASE; QUANTITATION LIMITS; SIGNAL/NOISE RATIO;

ENZYME ACTIVITY;

7 NITRO 2,1,3 BENZOXADIAZOLE; ASPARTIC ACID DERIVATIVE; DELTA SLEEP INDUCING PEPTIDE; METHYL GROUP; PROTEIN DEXTRO ASPARTATE METHYLTRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYST; CELL LYSATE; CELL STRAIN HEK293; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME MECHANISM; ENZYME SUBSTRATE; FLUOROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN METHYLATION; QUANTITATIVE ASSAY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TECHNIQUE;

EID: 56749150878     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.09.043     Document Type: Article

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