A robust method for proteomic characterization of mouse cartilage using solubility-based sequential fractionation and two-dimensional gel electrophoresis

Matrix Biology

Volumn 27, Issue 8, 2008, Pages 709-712

  Wilson, Richard ^a   Bateman, John F ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

2 D electrophoresis; Cartilage; Proteomics; Sample fractionation

Indexed keywords

CELL PROTEIN; GUANIDINE; SODIUM CHLORIDE;

ANIMAL EXPERIMENT; ARTICLE; CARTILAGE; CONTROLLED STUDY; EXTRACELLULAR MATRIX; FRACTIONATION; IMMUNOBLOTTING; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEOMICS; REPRODUCIBILITY; SOLUBILITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; CARTILAGE; CHEMICAL FRACTIONATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; MALE; MICE; MICE, INBRED C57BL; PROTEOMICS; SOLUBILITY;

EID: 56649120503     PISSN: 0945053X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.matbio.2008.07.007     Document Type: Article

References (13)

1. Belluoccio D., Wilson R., Thornton D.J., Wallis T.P., Gorman J.J., and Bateman J.F. Proteomic analysis of mouse growth plate cartilage. Proteomics 6 (2006) 6549-6553
2. Choe L.H., and Lee K.H. Quantitative and qualitative measure of intralaboratory two-dimensional protein gel reproducibility and the effects of sample preparation, sample load, and image analysis. Electrophoresis 24 (2003) 3500-3507
3. Goldring M.B., and Goldring S.R. Osteoarthritis. J. Cell. Physiol. 213 (2007) 626-634
4. Hendrickx S., Thomas P., Preston B.N., Stanton P.G., and Van Damme M.P. Partial characterization of matrix components interacting with cartilage proteoglycans. Arch. Biochem. Biophys. 390 (2001) 186-194
5. Hermansson M., Sawaji Y., Bolton M., Alexander S., Wallace A., Begum S., Wait R., and Saklatvala J. Proteomic analysis of articular cartilage shows increased type II collagen synthesis in osteoarthritis and expression of inhibin betaA (activin A), a regulatory molecule for chondrocytes. J. Biol. Chem. 279 (2004) 43514-43521
6. Meyer H.E., and Stuhler K. High-performance proteomics as a tool in biomarker discovery. Proteomics 7 Suppl. 1 (2007) 18-26
7. Newman B., and Wallis G.A. Skeletal dysplasias caused by a disruption of skeletal patterning and endochondral ossification. Clin. Genet. 63 (2003) 241-251
8. Pecora F., Forlino A., Gualeni B., Lupi A., Giorgetti S., Marchese L., Stoppini M., Tenni R., Cetta G., and Rossi A. A quantitative and qualitative method for direct 2-DE analysis of murine cartilage. Proteomics 7 (2007) 4003-4007
9. Righetti P.G., Castagna A., Antonioli P., and Boschetti E. Prefractionation techniques in proteome analysis: the mining tools of the third millennium. Electrophoresis 26 (2005) 297-319
10. Skandalis S.S., Theocharis D.A., Papageorgakopoulou N., and Vynios D.H. The extractability of extracellular matrix components as a marker of cartilage remodeling in laryngeal squamous cell carcinoma. Biochim. Biophys. Acta 1721 (2005) 81-88
11. Vincourt J.B., Lionneton F., Kratassiouk G., Guillemin F., Netter P., Mainard D., and Magdalou J. Establishment of a reliable method for direct proteome characterization of human articular cartilage. Mol. Cell. Proteomics 5 (2006) 1984-1995
12. Wilson R., Belluoccio D., and Bateman J.F. Proteomic analysis of cartilage proteins. Methods 45 (2008) 22-31
13. Wu J., Liu W., Bemis A., Wang E., Qiu Y., Morris E.A., Flannery C.R., and Yang Z. Comparative proteomic characterization of articular cartilage tissue from normal donors and patients with osteoarthritis. Arthritis Rheum. 56 (2007) 3675-3684