Capillary electrophoretic approach to screen for enzyme inhibitors in natural extracts

Journal of Chromatography A

Volumn 1053, Issue 1-2 SPEC. ISS., 2004, Pages 247-251

  Belenky, Alexei ^a   Hughes, Dallas ^a   Korneev, Alexei ^a   Dunayevskiy, Yuriy ^a  

^a Cetek Corproation   (United States)

Author keywords

Enzymatic assay; Mediated micro analysis; Protein tyrosine phosphatase

Indexed keywords

CAPILLARY ELECTROPHORESIS (CE); LIGANDS;

CAPILLARITY; ELECTROPHORESIS; REACTION KINETICS; SUBSTRATES; TITRATION;

ENZYMES;

PROTEIN TYROSINE PHOSPHATASE INHIBITOR;

AFFINITY CHROMATOGRAPHY; BINDING AFFINITY; CAPILLARY ELECTROPHORESIS; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME BINDING; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE; LIGAND BINDING; MICHAELIS MENTEN KINETICS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; SEPARATION TECHNIQUE; TITRIMETRY;

BIOLOGICAL FACTORS; ELECTROPHORESIS, CAPILLARY; ENZYME INHIBITORS;

EMMA;

EID: 5644277656     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2004.07.045     Document Type: Conference Paper

References (11)

1. F.A. Gomez, L.Z. Avila, Y.H. Chu, and G.M. Whitesides Anal. Chem. 66 1994 1785
2. N.H.H. Heegaard, S. Nilsson, and N.A. Guzman J. Chromatogr. B 715 1998 29
3. N.N.H. Heegaard, M.H. Nissen, and D.D.Y. Chen Electrophoresis 23 2002 815
4. D.E. Hughes, B.L. Karger, J.L. Waters, Y.M. Dunayevskiy, US Patent 6,432,651 B1 (August 13, 2002).
5. J.J. Bao, J.M. Fujima, and N.D. Danielson J. Chromatogr. B 699 1-2 1997 481
6. J. Wang Electrophoresis 23 2002 713
7. C.B. Cohen, E. Chin-Dixon, S. Jeong, and T.T. Nikiforov Anal. Biochem. 273 1999 89
8. P. Pascual, E. Martinez-Lara, J.A. Barcena, J. Lopez-Barea, and F. Toribio J. Chromatogr. 581 1992 49
9. J. Bao, and F.E. Regnier J. Chromatogr. 608 1992 217
10. Q. Xue, and E.S. Yeung Anal. Chem. 66 1994 1175
11. S. Van Dyck, E. Kaale, Z. Glatz, J. Hoogmartens, and A. Van Schepdael Electrophoresis 24 2003 3868