Neosappanone A, a xanthine oxidase (XO) inhibitory dimeric methanodibenzoxocinone with a new carbon skeleton from Caesalpinia sappan

Tetrahedron Letters

Volumn 45, Issue 46, 2004, Pages 8519-8522

  Nguyen, Mai Thanh Thi ^a   Awale, Suresh ^a   Tezuka, Yasuhiro ^a   Tran, Quan Le ^a   Kadota, Shigetoshi ^a  

^a UNIVERSITY OF TOYAMA   (Japan)

Author keywords

Caesalpinia sappan; Dimeric methanodibenzoxocinone; New carbon skeleton; Xanthine oxidase (XO) inhibitory activity

Indexed keywords

CAESALPINIA SAPPAN EXTRACT; METHANODIBENZOXOCINONE DERIVATIVE; NEOSAPPANONE A; PLANT EXTRACT; UNCLASSIFIED DRUG; XANTHINE OXIDASE INHIBITOR;

ARTICLE; CAESALPINIA; DRUG EFFECT; DRUG ISOLATION; DRUG STRUCTURE; ENZYME INHIBITION; IC 50; STRUCTURE ANALYSIS;

CAESALPINIA; CAESALPINIA SAPPAN;

EID: 5644235364     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tetlet.2004.09.107     Document Type: Article

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13. The XO inhibitory activity was assayed spectrophotometrically under aerobic conditions by using 96-well plates. The assay mixture consisting of 50 μL of test solution, 35 μL of 70 mM phosphate buffer (pH = 7.5), and 30 μL of enzyme solution (0.01 units/mL in 70 mM phosphate buffer, pH = 7.5) was prepared immediately before use. After preincubation at 25°C for 15 min, the reaction was initiated by the addition of 60 μL of substrate solution (150 μM xanthine in the same buffer). The assay mixture was incubated at 25°C for 30 min. The reaction was stopped by adding 25 μL of 1 N HCl, and the absorbance at 290 nm was measured with a Perkin-Elmer HTS-7000 Bio Assay Reader (Norwalk, CT, USA). A blank was prepared in the same way, but the enzyme solution was added to the assay mixture after adding 1 N HCl. One unit of XO is defined as the amount of enzyme required to produce 1 μmol of uric acid/min at 25°C