Genetic differentiation between two species of the medicinal leech, Hirudo medicinalis and the neglected H. verbana, based on random-amplified polymorphic DNA

Parasitology Research

Volumn 94, Issue 2, 2004, Pages 118-124

  Trontelj, Peter ^a   Sotler, Maruša ^a,b   Verovnik, Rudi ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

^b JOHNSON AND JOHNSON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HIRUDIN; MOLECULAR MARKER;

ARTICLE; GENE AMPLIFICATION; GENETIC POLYMORPHISM; GENETIC VARIABILITY; GEOGRAPHICAL VARIATION (SPECIES); GEOGRAPHY; LEECH; MORPHOLOGY; NONHUMAN; PRIORITY JOURNAL; SOUTHERN EUROPE; SPECIES; SPECIES DIFFERENTIATION; TAXON; TURKEY (REPUBLIC);

ANIMALS; DNA; HIRUDO MEDICINALIS; LEECHES; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; VARIATION (GENETICS);

HIRUDINIDA; HIRUDO MEDICINALIS; INVERTEBRATA;

EID: 5644221467     PISSN: 09320113     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00436-004-1181-x     Document Type: Article

References (38)

1. Black IVBW (1997) FORTRAN program to estimate F(ST) and effective migration rates among subpopulations using RAPD-PCR files. Colorado State University, Fort Collins, Colo
2. Caccone A, Allegrucci G, Fortunato C, Sbordoni V (1997) Genetic differentiation within the European sea bass (D. labrax) as revealed by RAPD- PCR Assay. J Hered 88:316-324
3. Callejas C, Ochando MD (2001) Molecular identification (RAPD) of the eight species of the genus Barbus (Cyprinidae) in the Iberian Peninsula. J Fish Biol 59:1589-1599
4. Castagnone-Sereno P, Vanlerberghe-Masutti F, Leroy F (1994) Genetic polymorphism between and within Meloidgyne species detected with RAPD markers. Genome 37:904-909
5. Chalmers KJ, Waugh R, Sprent JI, Simons AJ, Powell W (1992) Detection of genetic variation between and within populations of Gliricida sepium and G. maculata using RAPD markers. Heredity 69:465-472
6. Chapco W, Ashton NW, Martel RKB, Antonishyn N (1992) A feasibility study of random amplified polymorphic DNA in the population genetics and systematics of grasshoppers. Genome 35:569-574
7. Comincini S, Sironi M, Bandi C, Giunta C, Rubini M, Fontana F (1996) RAPD analysis of systematic relationships among the Cervidae. Heredity 76:215-221
8. Demirsoy A, Kasparek M, Akbulut A, Durmus Y, Akbulut NE, Çalskan M (2001) Phenology of the medicinal leech, Hirudo medicinalis L., in north-western Turkey. Hydrobiologia 462:19-24
9. Elliott JM, Tullett PA (1984) The status of the medicinal leech Hirudo medicinalis in Europe and especially in the British Isles. Biol Conserv 29:15-26
10. Excoffier L (1995) WINAMOVA, version 1.55. University of Geneva, Geneva
11. Excoffier L, Smouse PE, Quattro JM, (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479-491
12. Felsenstein J, (1993) PHYLIP (phylogeny inference package), version 3.57c. University of Washington, Seattle, Wash.
13. Gabrielsen TM, Bachmann K, Jakobsen KS, Brochmann C (1997) Glacial survival does not matter: RAPD phylogeography of Nordic Saxifraga oppositifolia. Mol Ecol 6:831-642
14. Goodwin SB, Legard DE, Smart CD, Levy M, Fry WE (1999) Gene flow analysis of molecular markers confirms that Phytophtora mirabilis and P. infestans are separate species. Mycologia 91:796-810
15. Hechtel FOP, Sawyer RT (2002) Toward a taxonomic revision of the medicinal leech Hirudo medicinalis Linnaeus, 1758 (Hirudinea: Hirudinidae): re-description of Hirudo troctina Johnston, 1816 from North Africa. J Nat Hist 36:1269-1289
16. Huff DR, Peakall R, Smouse PE (1993) RAPD variation within and among natural populations of outcrossing buffalograss [Buchloè dactyloides (Nutt.) Engelm.]. Theor Appl Genet 86:119-132
17. Kasparek M, Demirsoy A, Akbulut A, Akbulut NE, Çalskan M, Durmuç Y (2000) Distribution and status of the medicinal leech (Hirudo medicinalis L.) in Turkey. Hydrobiologia 441:37-44
18. Kazan K, Manners JM, Cameron DF (1993) Genetic relationships and variation in the Stylosanthes guianensis species complex assessed by RAPD. Genome 36:43-49
19. Lowe AJ, Gillies ACM, Wilson J, Dawson K (2000) Conservation genetics of bush mango from central/west Africa: implications from random amplified polymorphic DNA analysis. Mol Ecol 9:831-841
20. Lukin EI (1976) Piyavki, vol 1. (Fauna SSSR) Academy of Sciences of the SSSR, Leningrad
21. Maia Da Silva F, Rodrigues AC, Campaner M, Takata CSA, Brigido MC, Junqueira ACV, Coura JR, Takeda GF, Shaw JJ, Teixeira MMG (2004): Randomly amplified polymorphic DNA analysis of Trypanosoma rangeli and allied species from human, monkeys and other sylvatic mammals of the Brazilian Amazon disclosed a new group and a species-specific marker. Parasitology 128:283-294
22. Martin-Sanchez J, Diaz M, Artacho ME, Valero A (2003) Molecular arguments for considering Hysterothylacium fabri (Nematoda: Anisakidae) a complex of sibling species. Parasitol Res 89:214-220
23. Moquin-Tandon A (1846) Monographie de la Famille des Hirudineés. Bailliére, Paris
24. Nesemann H, Neubert E (1999) Annelida: Clitellata: Branchiobdellida, Acanthobdellea, Hirudinea. Spektrum, Berlin
25. Parsons YM, Shaw KL (2001) Species boundaries and genetic diversity among Hawaiian crickets of the genus Laupala identified using amplified fragment length polymorphism. Mol Ecol 10:1765-1772
26. Rohlf JF (1994) NTSYS. Numerical taxonomy and multivariate analysis system. Exeter Software, New York
27. Sawyer RT (1981) Why we need to save the medicinal leech. Oryx 16:165-168
28. Sawyer RT (1986) Leech biology and behaviour. Oxford University Press, Oxford
29. Schmidt H, Westheide W (2000) Are the meiofaunal polychaetes Hesionides arenaria and Stygocapitella subterranea true cosmopolitan species? Results of RAPD-PCR investigations. Zool Scr 29:17-27
30. Smith JJ, Scot-Craig JS, Leadbetter JR, Bush GL, Roberts DL, Fulbright DW (1994) Characterization of RAPD products from Xantomonas campestris and some comments on the use of RAPD products in phylogenetic analysis. Mol Phylogenet Evol 3:135-145
31. Sohn JH, Kang HA, Rao KJ, Kim CH, Choi ES, Chung BH, Rhee SK (2001) Current status of the anticoagulant hirudin: its biotechnological production and clinical practice. Appl Microbiol Biotechnol 57:606-613
32. Thormann CE, Ferreira ME, Camargo LEA, Tivang JG, Osborn TC (1994) Comparison of RFLP and RAPD markers to estimate genetic relationship within and among cruciferous species. Theor Appl Genet 88:973-980
33. Verovnik R, Trontelj P, Sket B (1999) Genetic differentiation and species status within the snail leech Glossiphonia complanata aggregate (Hirudinea: Glossiphoniidae) revealed by RAPD analysis. Arch Hydrobiol 144:327-338
34. Wells S, Coombes W (1987) The status of and trade in the medicinal leech. Traffic Bull 8:64-69
35. Wells SM, Pyle RM, Collins NM (1983) The IUCN invertebrate red data book. The World Conservation Union, Cambridge
36. Westheide W, Hass-Cordes E (2001) Description of a cryptic Petitia species (Polychaeta: Syllidae) from the island of Mahe (Seychelles, Indian Ocean) using RAPD markers and ITS2 sequences. J Zool Syst Evol Res 39:103-111
37. Whitaker IS, Rao J, Izadi D, Butler PE (2004) Hirudo medicinalis: ancient origins of, and trends in the use of medicinal leeches throughout history. Br J Oral Maxillofac Surg 42:133-137
38. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey ST (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531-6535