Low sensitivity of the comet assay to detect acetaldehyde-induced genotoxicity

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 657, Issue 2, 2008, Pages 93-97

  Speit, Günter ^a   Fröhler Keller, Manuela ^a   Schütz, Petra ^a   Neuss, Simone ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Comet assay; Cytotoxicity; DNA protein cross links; Micronuclei; Sister chromatid exchange

Indexed keywords

ACETALDEHYDE; PROTEINASE K;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL POPULATION; COMET ASSAY; CONCENTRATION RESPONSE; CYTOTOXICITY; DNA CROSS LINKING; DNA DAMAGE; DNA STRAND BREAKAGE; GAMMA RADIATION; GENE INDUCTION; GENOTOXICITY; MICRONUCLEUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; REPRODUCIBILITY; SENSITIVITY ANALYSIS; SISTER CHROMATID EXCHANGE; STATISTICAL ANALYSIS;

ACETALDEHYDE; ANIMALS; CELLS, CULTURED; CHO CELLS; COMET ASSAY; CRICETINAE; CRICETULUS; CROSS-LINKING REAGENTS; DNA DAMAGE; MICRONUCLEUS TESTS; SENSITIVITY AND SPECIFICITY; SISTER CHROMATID EXCHANGE;

MAMMALIA;

EID: 56249128207     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2008.07.010     Document Type: Article

References (29)

1. Barker S., Weinfeld M., and Murray D. DNA-protein crosslinks: their induction, repair, and biological consequences. Mutat. Res. 589 (2005) 111-135
2. Speit G., Schutz P., Hogel J., and Schmid O. Characterization of the genotoxic potential of formaldehyde in V79 cells. Mutagenesis 22 (2007) 387-394
3. Speit G., Neuss S., Schutz P., Frohler-Keller M., and Schmid O. The genotoxic potential of glutaraldehyde in mammalian cells in vitro in comparison with formaldehyde. Mutat. Res. 649 (2008) 146-154
4. Kuykendall J.R., and Bogdanffy M.S. Efficiency of DNA-histone crosslinking induced by saturated and unsaturated aldehydes in vitro. Mutat. Res. 283 (1992) 131-136
5. Lambert B., Chen Y., He S.M., and Sten M. DNA cross-links in human leucocytes treated with vinyl acetate and acetaldehyde in vitro. Mutat. Res. 146 (1985) 301-303
6. He S.M., and Lambert B. Induction and persistence of SCE-inducing damage in human lymphocytes exposed to vinyl acetate and acetaldehyde in vitro. Mutat. Res. 158 (1985) 201-208
7. Helander A., and Lindahl-Kiessling K. Increased frequency of acetaldehyde-induced sister-chromatid exchanges in human lymphocytes treated with an aldehyde dehydrogenase inhibitor. Mutat. Res. 264 (1991) 103-107
8. Mechilli M., Schinoppi A., Kobos K., Natarajan A.T., and Palitti F. DNA repair deficiency and acetaldehyde-induced chromosomal alterations in CHO cells. Mutagenesis 23 (2008) 51-56
9. Singh N.P., and Khan A. Acetaldehyde: genotoxicity and cytotoxicity in human lymphocytes. Mutat. Res. 337 (1995) 9-17
10. Blasiak J., Trzeciak A., Malecka-Panas E., Drzewoski J., and Wojewodzka M. In vitro genotoxicity of ethanol and acetaldehyde in human lymphocytes and the gastrointestinal tract mucosa cells. Toxicol. In Vitro 14 (2000) 287-295
11. Merk O., and Speit G. Detection of crosslinks with the comet assay in relationship to genotoxicity and cytotoxicity. Environ. Mol. Mutagen. 33 (1999) 167-172
12. Greenwood S.K., Hill R.B., Sun J.T., Armstrong M.J., Johnson T.E., Gara J.P., and Galloway S.M. Population doubling: a simple and more accurate estimation of cell growth suppression in the in vitro assay for chromosomal aberrations that reduces irrelevant positive results. Environ. Mol. Mutagen. 43 (2004) 36-44
13. Hu Y., Kabler S.L., Tennant A.H., Townsend A.J., and Kligerman A.D. Induction of DNA-protein crosslinks by dichloromethane in a V79 cell line transfected with the murine glutathione-S-transferase theta 1 gene. Mutat. Res. 607 (2006) 231-239
14. Merk O., and Speit G. Significance of formaldehyde-induced DNA-protein crosslinks for mutagenesis. Environ. Mol. Mutagen. 32 (1998) 260-268
15. Speit G., Schutz P., and Merk O. Induction and repair of formaldehyde-induced DNA-protein crosslinks in repair-deficient human cell lines. Mutagenesis 15 (2000) 85-90
16. Olive P.L., Wlodek D., Durand R.E., and Banath J.P. Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res. 198 (1992) 259-267
17. Pfuhler S., and Wolf H.U. Detection of DNA-crosslinking agents with the alkaline comet assay. Environ. Mol. Mutagen. 27 (1996) 196-201
18. Tice R.R., Yager J.W., Andrews P., and Crecelius E. Effect of hepatic methyl donor status on urinary excretion and DNA damage in B6C3F1 mice treated with sodium arsenite. Mutat. Res. 386 (1997) 315-334
19. Hartley J.M., Spanswick V.J., Gander M., Giacomini G., Whelan J., Souhami R.L., and Hartley J.A. Measurement of DNA cross-linking in patients on ifosfamide therapy using the single cell gel electrophoresis (comet) assay. Clin. Cancer Res. 5 (1999) 507-512
20. Corrie P.G., Shaw J., Spanswick V.J., Sehmbi R., Jonson A., Mayer A., Bulusu R., Hartley J.A., and Cree I.A. Phase I trial combining gemcitabine and treosulfan in advanced cutaneous and uveal melanoma patients. Br. J. Cancer 92 (2005) 1997-2003
21. Speit G., and Merk O. Evaluation of mutagenic effects of formaldehyde in vitro: detection of crosslinks and mutations in mouse lymphoma cells. Mutagenesis 17 (2002) 183-187
22. Schmid O., and Speit G. Genotoxic effects induced by formaldehyde in human blood and implications for the interpretation of biomonitoring studies. Mutagenesis 22 (2007) 69-74
23. Grafstrom R.C., Dypbukt J.M., Sundqvist K., Atzori L., Nielsen I., Curren R.D., and Harris C.C. Pathobiological effects of acetaldehyde in cultured human epithelial cells and fibroblasts. Carcinogenesis 15 (1994) 985-990
24. Hecht S.S., McIntee E.J., and Wang M. New DNA adducts of crotonaldehyde and acetaldehyde. Toxicology 166 (2001) 31-36
25. Marinari U.M., Ferro M., Sciaba L., Finollo R., Bassi A.M., and Brambilla G. DNA-damaging activity of biotic and xenobiotic aldehydes in Chinese hamster ovary cells. Cell Biochem. Funct. 2 (1984) 243-248
26. Kuykendall J.R., and Bogdanffy M.S. Reaction kinetics of DNA-histone crosslinking by vinyl acetate and acetaldehyde. Carcinogenesis 13 (1992) 2095-2100
27. Grafstrom R.C. In vitro studies of aldehyde effects related to human respiratory carcinogenesis. Mutat. Res. 238 (1990) 175-184
28. Costa M., Zhitkovich A., Harris M., Paustenbach D., and Gargas M. DNA-protein cross-links produced by various chemicals in cultured human lymphoma cells. J. Toxicol. Environ. Health 50 (1997) 433-449
29. Olin K.L., Cherr G.N., Rifkin E., and Keen C.L. The effects of some redox-active metals and reactive aldehydes on DNA-protein cross-links in vitro. Toxicology 110 (1996) 1-8