Validation of the design of feeding experiments involving [14C]substrates used to monitor metabolic flux in higher plants

Phytochemistry

Volumn 69, Issue 17, 2008, Pages 2920-2927

  Harrison, Peter W ^a   Kruger, Nicholas J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Cyano 4 hydroxycinnamic acid; 14CO2 capture (alkaline trap); 1 14C gluconate; 14C glucose metabolism; Arabidopsis thaliana; Brassicaceae; Nitrite; Respirometric 14CO2 release

Indexed keywords

CARBON; CARBON DIOXIDE; GLUCOSE;

ARABIDOPSIS; ARTICLE; CELL CULTURE; CYTOLOGY; GENE EXPRESSION REGULATION; METABOLISM; OXIDATION REDUCTION REACTION; PHYSIOLOGY; REPRODUCIBILITY;

ARABIDOPSIS; CARBON DIOXIDE; CARBON RADIOISOTOPES; CELLS, CULTURED; GENE EXPRESSION REGULATION, PLANT; GLUCOSE; OXIDATION-REDUCTION; REPRODUCIBILITY OF RESULTS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; BRASSICACEAE; EMBRYOPHYTA;

EID: 56249106118     PISSN: 00319422     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.phytochem.2008.09.024     Document Type: Article

References (37)

1. ap Rees T. Contribution of metabolic pathways to respiration. In: Davies D.D. (Ed). The Biochemistry of Plants: A Comprehensive Treatise (1980), Academic Press, New York, London 1-29
2. ap Rees T., Fuller W.A., and Green J.H. Extremely high activities of phosphophenolpyruvate carboxylase in thermogenic tissues of Araceae. Planta 152 (1981) 79-86
3. Averill R.H., Bailey-Serres J., and Kruger N.J. Co-operation between cytosolic and plastidic oxidative pentose phosphate pathways revealed by 6-phosphogluconate dehydrogenase-deficient genotypes of maize. Plant J. 14 (1998) 449-457
4. Axelrod B., and Beevers H. Mechanisms of carbohydrate breakdown in plants. Annu. Rev. Plant Physiol. 7 (1956) 267-298
5. Bassham J.A., and Calvin M. The Path of Carbon in Photosynthesis (1957), Prentice-Hall Englewood Cliffs, New Jersey
6. Beevers H. Respiratory Metabolism in Plants (1961), Row Peterson and Co., Evanston, IL pp. 232
7. Bindon K.A., and Botha F.C. Carbon allocation to the insoluble fraction, respiration and triose-phosphate cycling in the sugarcane culm. Physiol. Plant. 116 (2002) 12-19
8. 2 in roots of Pisum and Plantago. Phytochemistry 24 (1985) 1635-1638
9. Butt V.S., and Beevers H. The regulation of pathways of glucose catabolism in maize roots. Biochem. J. 80 (1961) 21-27
10. Centeno D.C., Oliver S.N., Nunes-Nesi A., Geigenberger P., Machado D.N., Loureiro M.E., Silva M.A., and Fernie A.R. Metabolic regulation of pathways of carbohydrate oxidation in potato (Solanum tuberosum) tubers. Physiol. Plant. 133 (2008) 744-754
11. Cohen J. A power primer. Psychol. Bull. 70 (1992) 426-443
12. Davies D.D., Giovanelli J., and ap Rees T. Plant Biochemistry (1964), Blackwell Scientific, Oxford pp. 120-122
13. Davies H.V., Shepherd L.V., Burrell M.M., Carrari F., Urbanczyk-Wochniak E., Leisse A., Hancock R.D., Taylor M., Viola R., Ross H., McRae D., Willmitzer L., and Fernie A.R. Modulation of fructokinase activity of potato (Solanum tuberosum) results in substantial shifts in tuber metabolism. Plant Cell Physiol. 46 (2005) 1103-1115
14. Dry I.B., and Wiskich J.T. Inhibition of 2-oxoglutarate oxidation in plant mitochondria by pyruvate. Biochem. Biophys. Res. Commun. 133 (1985) 397-403
15. Edwards S., Nguyen B.-T., Do B., and Roberts J.K.M. Contribution of malic enzyme, pyruvate kinase, phosphoenolpyruvate carboxylase and the Krebs cycle to respiration and biosynthesis and to intracellular pH regulation during hypoxia in maize root tips observed by nuclear magnetic resonance and gas chromatography-mass spectrometry. Plant Physiol. 116 (1998) 1073-1081
16. 14C]gluconate. Planta 216 (2002) 265-272
17. Geigenberger P., Regierer B., Nunes-Nesi A., Leisse A., Urbanczyk-Wochniak E., Springer F., van Dongen J.T., Kossmann J., and Fernie A.R. Inhibition of de novo pyrimidine synthesis in growing potato tubers leads to a compensatory stimulation of the pyrimidine salvage pathway and a subsequent increase in biosynthetic performance. Plant Cell 17 (2005) 2077-2088
18. Gibbs M., and Beevers H. Glucose dissimilation in the higher plant: effect of age and tissue. Plant Physiol. 30 (1955) 343-347
19. Goffman F.D., Alonso A.P., Schwender J., Shachar-Hill Y., and Ohlrogge J.B. Light enables a very high efficiency of carbon storage in developing embryos of rapeseed. Plant Physiol. 138 (2005) 2269-2279
20. Halestrap A.P. The mitochondrial pyruvate carrier. Kinetics and specificity for substrates and inhibitors. Biochem. J. 148 (1975) 85-96
21. 13C-substrates on metabolism in Arabidopsis thaliana cell suspension cultures. Phytochemistry 68 (2007) 2176-2188
22. Malone J.G., Mittova V., Ratcliffe R.G., and Kruger N.J. The response of carbohydrate metabolism in potato tubers to low temperature. Plant Cell Physiol. 47 (2006) 1309-1322
23. May M.J., and Leaver C.J. Oxidative stimulation of glutathione synthesis in Arabidopsis thaliana suspension cultures. Plant Physiol. 103 (1993) 621-627
24. Morgan M.J., Lehmann M., Schwarzländer M., Baxter C.J., Sienkiewicz-Porzucek A., Williams T.C.R., Schauer N., Fernie A.R., Fricker M.D., Ratcliffe R.G., Sweetlove L.J., and Finkemeier I. Decrease in manganese superoxide dismutase leads to reduced root growth and affects tricarboxylic acid cycle flux and mitochondrial redox homeostasis. Plant Physiol. 147 (2008) 101-114
25. Motulsky H. Intuitive Biostatistics (1995), Oxford University Press, Oxford pp. 285-286
26. Murashige T., and Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15 (1962) 473-497
27. Nunes-Nesi A., Carrari F., Lytovchenko A., Smith A.M., Loureiro M.E., Ratcliffe R.G., Sweetlove L.J., and Fernie A.R. Enhanced photosynthetic performance and growth as a consequence of decreasing mitochondrial malate dehydrogenase activity in transgenic tomato plants. Plant Physiol. 137 (2005) 611-622
28. Oliver S.N., Tiessen A., Fernie A.R., and Geigenberger P. Decreased expression of plastidial adenylate kinase in potato tubers results in an enhanced rate of respiration and a stimulation of starch synthesis that is attributable to post-translational redox-activation of ADP-glucose pyrophosphorylase. J. Exp. Bot. 59 (2008) 315-325
29. Runquist M., and Kruger N.J. Control of gluconeogenesis by isocitrate lyase in endosperm of germinating castor bean seedlings. Plant J. 19 (1999) 423-431
30. Schwender J., Goffman F., Ohlrogge J.B., and Shachar-Hill Y. A novel role for Rubisco in the carbon economy of developing seeds. Nature 432 (2004) 779-782
31. Scott P., and Kruger N.J. Influence of elevated fructose-2,6-bisphosphate levels on starch mobilization in transgenic tobacco leaves in the dark. Plant Physiol. 108 (1995) 1569-1577
32. Sienkiewicz-Porzucek A., Nunes-Nesi A., Sulpice R., Lisec J., Centeno D.C., Carillo P., Leisse A., Urbanczyk-Wochniak E., and Fernie A.R. Mild reductions in mitochondrial citrate synthase activity result in a compromised nitrate assimilation and reduced leaf pigmentation but have no effect on photosynthetic performance or growth. Plant Physiol. 147 (2008) 115-127
33. Stitt M., and ap Rees T. Pathways of carbohydrate oxidation in leaves of Pisum sativum and Triticum aestivum. Phytochemistry 17 (1978) 1251-1256
34. Studart-Guimarães C., Fait A., Nunes-Nesi A., Carrari F., Usadel B., and Fernie A.R. Reduced expression of succinyl CoA ligase can be compensated for by an upregulation of the γ-amino-butyrate (GABA) shunt in illuminated tomato leaves. Plant Physiol. 145 (2007) 626-639
35. Wardlaw A.C. Practical Statistics for Experimental Biologists (1985), Wiley, Chichester pp. 180-181
36. 13C metabolic flux analysis with sole quantification of carbon dioxide labelling. Comput. Biol. Chem. 29 (2005) 121-133
37. Yang T.H., Wittmann C., and Heinzle E. Respirometric 13C flux analysis-Part II: in vivo flux estimation of lysine-producing Corynebacterium glutamicum. Metab. Eng. 8 (2006) 432-446