Cysteine-390 is the binding site of luminous substance with symplectin, a photoprotein from Okinawan squid, Symplectoteuthis oualaniensis

Proceedings of the Japan Academy Series B: Physical and Biological Sciences

Volumn 84, Issue 9, 2008, Pages 386-392

  Isobe, Minoru ^a   Kuse, Masaki ^a   Tani, Naoki ^a   Fujii, Tatsuya ^a   Matsuda, Tsukasa ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

Binding site; Fluoro dehydrocoelenterazine; Luminous substrate; Nano LC MS; Photoprotein; Symplectin

Indexed keywords

CYSTEINE; LUMINESCENT AGENT; PHOTOPROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CEPHALOPOD; CHEMISTRY; ENZYME ACTIVE SITE; LUMINESCENCE; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS;

AMINO ACID SEQUENCE; ANIMALS; CATALYTIC DOMAIN; CYSTEINE; DECAPODIFORMES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; LUMINESCENCE; LUMINESCENT AGENTS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA;

CEPHALOPODA; SYMPLECTOTEUTHIS OUALANIENSIS;

EID: 56249090758     PISSN: 03862208     EISSN: 13492896     Source Type: Journal    
DOI: 10.2183/pjab.84.386     Document Type: Article

References (15)

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11. Isobe, M. and Matsuda, T. (2000) JP Patent 2000-154786. Accession number of symplectin is AB447990. The S-S bondings are located between Cysteines 92-110, 129-137, and 380-385, respectively. The details of these informations are to be reported elsewhere in due course.
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14. These conditions are essentially identical as reported in experimental part in Ref. 5 and following paper; Isobe, M., Kai, H., Kurahashi, T., Suwan, S., Pitchayawasin-T. S., Franz, T., Tani, N., Higashi, K. and Nishida, H. (2006) The molecular mechanism of the termination of insect diapause, Part 1: A timer protein, TIME-EA4, in the diapause eggs of the silkworm Bombyx mori is a Metallo-Glycoprotein. ChemBioChem. 7, 1590-1598.
15. These conditions are essentially identical as reported in Refs. 4 and 7.