Template-independent DNA polymerases

Current Protocols in Molecular Biology

Volumn , Issue SUPPL. 84, 2008, Pages

  Yue, Dongxian ^a   Tabor, Stanley ^b   Nichols, Nicole M ^a  

^a NEW ENGLAND BIOLABS   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

DNA 3 end labeling; DNA tailing; TdT; Terminal deoxynucleotidyl transferase; TUNEL assay

Indexed keywords

DEOXYRIBONUCLEOTIDE; DNA FRAGMENT; DNA NUCLEOTIDYLEXOTRANSFERASE; DNA POLYMERASE; PHOSPHATE; PHOSPHORUS 32; NUCLEOTIDE; CELL DNA; POLYDEOXYRIBONUCLEOTIDE; POLYMER;

APOPTOSIS; CATALYSIS; DNA DAMAGE; DNA MODIFICATION; DNA SEQUENCE; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ISOTOPE LABELING; MOLECULAR CLONING; NICK END LABELING; PRIORITY JOURNAL; REVIEW; ARTICLE; METABOLISM; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; 3' UNTRANSLATED REGION; DNA DETERMINATION; DNA FRAGMENTATION;

CLONING, MOLECULAR; DNA NUCLEOTIDYLEXOTRANSFERASE; IN SITU NICK-END LABELING; NUCLEIC ACID AMPLIFICATION TECHNIQUES; NUCLEOTIDES; TEMPLATES, GENETIC;

EID: 56149107032     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb0306s84     Document Type: Review

References (5)

1. Deng, G.R. and Wu, R. 1983. Terminal transferase: Use in the tailing of DNA and for in vitro mutagenesis. Methods Enzymol.#1i] 100:96-116.
2. Labat-Moleur, F., Guillermet, C., Lorimier, P., Robert, C., Lantuejoul, S., Brambilla, E., and Negoescu, A. 1998. TUNEL apoptotic cell detection in tissue sections: Critical evaluation and improvement. J. Histochem. Cytochem. 46:327-334.
3. Rohwer, F. and Azam, F. 2000. Detection of DNA damage in prokaryotes by terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling. Appl. Environ. Microbiol. 66:1001-1006.
4. 32P]cordycepin-5′-triphosphate. Gene 10:177-183.
5. Vincent, C., Tchen, P., Cohen-Solal, M., and Kourilsky, P. 1982. Synthesis of 8-(2,4-dinitrophenyl-2,6-aminohexyl) aminoadenosine-5′-triphosphate: Biological properties and potential uses. Nucleic Acids Res. 10:6787-6796.