Diagnostic multiplex polymerase chain reaction assay for the identification of Pseudomonas aeruginosa from the skin biopsy specimens in burn wound infections and detection of antibiotic susceptibility

Saudi Medical Journal

Volumn 29, Issue 8, 2008, Pages 1109-1114

  Mashouf, Rasoul Y ^a   Zamani, Alireza ^a   Farahani, Hadi S ^a  

^a HAMADAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

[No Author keywords available]

Indexed keywords

AMIKACIN; ANTIBIOTIC AGENT; AZTREONAM; BACTERIAL PROTEIN; CARBENICILLIN; CEFALOTIN; CEFEPIME; CEFOTAXIME; CEFTAZIDIME; CIPROFLOXACIN; GENTAMICIN; IMIPENEM; MEROPENEM; OUTER MEMBRANE LIPOPROTEIN I; OUTER MEMBRANE LIPOPROTEIN L; PIPERACILLIN PLUS TAZOBACTAM; TETRACYCLINE; TIMENTIN; TOBRAMYCIN; UNCLASSIFIED DRUG;

ADULT; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BURN INFECTION; CONTROLLED STUDY; CROSS-SECTIONAL STUDY; DIAGNOSTIC VALUE; DISK DIFFUSION; DRUG EFFICACY; FEMALE; GENE AMPLIFICATION; HOSPITAL ADMISSION; HUMAN; HUMAN TISSUE; IRAN; MAJOR CLINICAL STUDY; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; SENSITIVITY ANALYSIS; SKIN BIOPSY; ANTIBIOTIC RESISTANCE; BIOPSY; BURN; GRAM NEGATIVE INFECTION; ISOLATION AND PURIFICATION; MICROBIOLOGY; POLYMERASE CHAIN REACTION; SKIN; WOUND INFECTION;

ADULT; BIOPSY; BURNS; CROSS-SECTIONAL STUDIES; DRUG RESISTANCE, BACTERIAL; FEMALE; GENE AMPLIFICATION; HUMANS; MALE; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; SKIN; WOUND INFECTION;

EID: 56149089661     PISSN: 03795284     EISSN: 16583175     Source Type: Journal    
DOI: None     Document Type: Article

References (42)

1. Fonseca AP, Correia P, Sousa JC, Tenreiro R. Association patterns of Pseudomonas aeruginosa clinical isolates as reserved by virulence traits, antibiotic resistance, serotype and genotype. FEMS Immunol Med Microbiol 2007; 51: 505-516.
2. Gad GF, El-Domany RA, Zaki S, Ashour HM. Characterization of Pseudomonas aeruginosa isolated from clinical and environmental samples in Minia, Egypt: Prevalence, antibiogram and resistance mechanism. J Antimicrob Chemother 2007; 60: 1010-1017.
3. Ekrami A, Kalantar E. Bacterial infections in burn patients at a burn hospital in Iran. Indian J Med Res 2007; 126: 541-544.
4. Oudhuis GJ, Verbon A, Hoogkamp-Korstanje JA, Stobberingh EE; Susceptibility Surveillance Study Group. Antimicrobial resistance in Escherichia coli and Pseudomonas aeruginosa from Intensive Care Units in The Netherlands, 1998-2005. Int J Antimicrob Agents 2008; 31: 59-63.
5. Palleroni NJ, Pseudomonas. In: Borriello SP, Murray PR, Funke G, editors. Topley and Wilson's Microbiology and Microbial. Infections, Bacteriology. 10th edition. London: Hodder Arnold, ASM Press 2005. p. 1591-1598.
6. Agnihotri N, Gupta V, Joshi RM. Aerobic bacterial isolates from burn wound infections and their antibiograms-a five-year study. Burns 2004; 30: 241-243.
7. Lamia, Bousselmi K, Saida BR, Allah MA. Epidemiological profile and antibiotic susceptibility of Pseudomonas aeruginosa isolates within the burned patient hospitalized in the intensive care burn unit. Tunis Med 2007; 85: 124-127.
8. Orrett FA. Antimcrobial susceptibility survey of Pseudomonas aeruginosa strains isolated from clinical sources. J Natl Med Assoc 2004; 96: 1065-1069.
9. Lawrence JC. Burn bacteriology during the last 50 years. Burn 1992; 18: S23-S29.
10. Vindenes H, Bjerknes R. Microbial colonization of large wounds. Burns 1995; 21: 575-579.
11. De Macedo JL, Santos JB. Bacterial and fungal colonization of burn wounds. Mem Inst Oswaldo Cruz 2005; 100: 535-359.
12. Vindenes H, Bjerknes R. Microbial colonization of large wounds. Burns 1995; 21: 575-579.
13. Harris A, Torres-Viera C, Venkataraman L, DeGirolami L, Samore M, Carmeli Y. Epidemiology and clinical outcomes of patients with multiresistant Pseudomonas aeruginosa. Clin Infect Dis 1999; 28: 1128-1133.
14. Wareham DW, Curtis MA. A genotypic and phenotypic comparison of type III secretion profiles of Pseudomonas aeruginosa cystic fibrosis and bacteremia isolates. Int J Med Microbiol 2007; 297: 227-234.
15. Lee YC, Ahn BJ, Jin JS, Kim JU, Lee SH, Song do Y, et al. Molecular characterization of Pseudomonas aeruginosa isolates resistant to all antimicrobial agents, but susceptible to colistin, in Daegu, Korea. J Microbiol 2007; 45: 358-363.
16. Mendes RE, Kiyota KA, Monterio J, Castanheira M, Andrade SS, Gales AC, et al. Rapid detection and identification of metallo-beta-lactamase-encoding genes by multiplex real-time PCR assay and melt curve analysis. J Clin Microbiol 2007; 45: 544-547.
17. Lavenir R, Jocktane D, Laurent F, Nazaret S, Cournoyer B. Improved reliability of Pseudomonas aeruginosa PCR detection by the use of the species-specific ecfX gene target. J Microbiol Methods 2007; 70: 20-29.
18. Nikaido H. Prevention of drug access to bacterial targets: Permeability barriers and active efflux. Science 1994; 264: 382-388.
19. Livermore DM. Of Pseudomonas, porins, pumps and carbapenems. J Antimicrob Chemother 2001; 47: 247-250.
20. Cornelis, Bouia A, Belarbi A, Guyonvarch A, Kammerer B, Hannaert V. et al. Cloning and analysis of the gene for the major outer membrane lipoprotein from Pseudomonas aeruginosa. Mol Microbiol 1989; 3: 421-428.
21. De Vos D, Lim A Jr, De Vos P, Sarniguet A, Kersters K, Cornelis P. Detection of the outer membrane lipoprotein I and its gene influorescent and non fluorescent pseudomonads: Implications for taxonomy and diagnosis. J Gen Microbiol 1993; 139: 2215-2223.
22. Hancock RE, Siehnel R, Martin N. Outer membrane proteins of Pseudomonas. Mol Microbiol 1990; 4: 1069-1075.
23. Lim A Jr, De Vos D, Brauns M, Mossialos D, Gaballa A, Qing D, et al. Molecular and immunological characterization of OprL, the 18 kDa outer-membrane peptidoglycan-associated lipoprotein (PAL) of Pseudomonas aeruginosa Microbiology 1997; 143: 1709-1716.
24. Sambrook J, Russell D. Molecular cloning: A laboratory manual. 3rd edition. New York (NY): Cold Spring Harbor Laboratory press, USA; 2001. p. 8.107.
25. Forbes BA, Sahm DF, Weissfeld AS. Bailey & Scott's Diagnostic microbiology. 11th Ed. Philadelphia (PA): Mosby Co; 2002. p. 368-375 and 204-213.
26. Clinical and Laboratory Standard Institute. Performance standards for antimicrobial disk susceptibility tests. NCCLS documents M 100 S15. Wayne, PA: Clinical and Laboratory Standard Institute, USA; 2005.
27. Nasser S, Mabrouk A, Maher A. Colonization of burn wounds in Ain Shams University burn unit. Burns 2003; 29: 229-233.
28. Komolafe OO, James J, Kalongolera L, Makoka M. Bacteriology of burns at the Queen Elizabeth Central Hospital, Blantyre, Malawi. Burns 2003; 29: 235-238.
29. Sharma BR, Harish D, Singh VP, Bangar S. Septicemia as a cause of death in burns: An autopsy study. Burns 2007; 33: 538-539.
30. Vindenes H, Bjerknes R. Microbial colonization of large wounds. Burns 1995; 21: 575-579.
31. Lesseva M, Hadjiiski OG. Staphylococcal infections in the Sofia Burn Centre, Bulgaria. Burns 1996; 22: 279-282.
32. Vostrugina K, Gudaviciene D, Vitkauskiene A. Bacteremias in patients with severe burn trauma. Medicina (Kaunas) 2006; 42: 576-579.
33. Messadi AA. Thabet L, Bouselmi K, Ben Redjeb S. A study of sensitivity to antibiotics for Staphylococcus aureus strains isolated in a department of resuscitation of badly burnt patients. Tunis Med 2006; 84: 74-77.
34. Abd, El-Haleem D, Kheiralla ZH, Zaki S, Rushdy AA, Abd-El-Rahiem W. Multiplex-PCR and PCR-RFLP assays in monitor water quality against pathogenic bacteria. J Environ Monit 2003; 5: 865-870.
35. Dabrowski W, Czeckajlo-Kolodziej U, Medrala D, Giedrys-Kalemba S, Optimisation of AP-PCR fingerprinting discriminatory power for clinical isolates of Pseudomonas aeriginosa. FEMS Microbiol Lett 2003;. 218: 51-57.
36. Taylor RF, Hodson ME, Pitt TL. Adult cystic fibrosis: Assocition of acute pulmonary exacerbation and incresing severity of lung disease with auxotrophic mutants of Pseudomonas aeruginosa. Thorax 1993; 48: 1002-1005.
37. Paramythiotou E, Lucet JC, Timsit JF, Vanjak D, Paugam-Burtz C, Trouillet JL, et al. Acquisition of multidrug-resistant Pseudomonas aeruginosa in patients in intensive care units: Role of antibiotics with antipseudomonal activity. Clin Infect Dis 2004; 38: 670-677.
38. Ouderkirk JP, Nord JA, Turett GS, Kislak JW. Polymyxin B nephrotoxicity and efficacy against nosocomial infections caused by multiresistant gram-negative bacteria. Antimicrob Agents Chemother 2003; 47:2659-2662.
39. Ivanov DV, Krapivina IV. Etiology of nosocomial surgical infections caused by gram-negative bacteria, and profile of their antibiotic: resistance. Zh Mikrobiol Epidemiol Immunobiol 2007, 5: 90-93.
40. Lockhart SR, Abramson MA, Beckmann SE, Gallagher G, Riedel S, Diekema DJ, et al. Antimicrobial resistance among Gram-negative bacilli causing infections in intensive care unit patients in the United States between 1993 and 2004. J Clin Microbiol 2007; 45: 3352-3359.
41. Rasmussen BA, Bush K, Carbapenem-hydrolyzing β-lactamases. Antimicrob. Agents Chemother 1997; 41: 223-232.
42. Rodriguez CN, Rodriguez-Morales AJ, Garcia A, Pastran B, Meijomil P. Antimicrobial resistance of Pseudomonas aeruginosa strains isolated from surgical infections in a 7-year period at a general hospital in Venezuela. Surg Infect (Larchmt) 2006; 7: 269-273.