Effect of glutathione (GSH) depletion on DNA damage and blood chemistry in aged and young rats

Journal of Toxicological Sciences

Volumn 33, Issue 4, 2008, Pages 421-429

  Hashimoto, Kazuyuki ^a,b,c   Takasaki, Wataru ^c   Yamoto, Takashi ^c   Manabe, Sunao ^c   Sato, Itaru ^a,b   Tsuda, Shuji ^a,b  

^a GIFU UNIVERSITY   (Japan)

^b IWATE UNIVERSITY   (Japan)

^c DAIICHI SANKYO CO   (Japan)

Author keywords

Aging; Comet assay; DNA damage; Glutathione; L buthionine (S,R) sulfoximine

Indexed keywords

ALANINE AMINOTRANSFERASE; ALBUMIN; ALKALINE PHOSPHATASE; ASPARTATE AMINOTRANSFERASE; BILIRUBIN; BUTHIONINE SULFOXIMINE; CHLORIDE; CHOLESTEROL; CREATININE; GLOBULIN; GLUTATHIONE; LEVO BUTHIONINE SULFOXIMINE; PHOSPHATE; SODIUM; TRIACYLGLYCEROL; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLOOD ANALYSIS; BLOOD CHEMISTRY; CONTROLLED STUDY; DNA DAMAGE; HISTOPATHOLOGY; NONHUMAN; RAT;

RATTUS;

EID: 55949127439     PISSN: 03881350     EISSN: 18803989     Source Type: Journal    
DOI: 10.2131/jts.33.421     Document Type: Article

References (30)

1. Cabelof, D.C., Raffoul, J.J., Ge, Y., Van Reimmen, H., Matherly, L.H. and Heydari, A.R. (2006): Age-related loss of the DNA repair response following exposure to oxidative stress. J. Gerontol. A. Biol. Sci. Med. Sci., 61, 427-434.
2. Chung, F.L., Komninou, D., Zhang, L., Nath, R., Pan, J., Amin, S. and Richie, J. (2005): Glutathione depletion enhances the formation of endogenous cyclic DNA adducts derived from t-4-hydroxy-2-nonenal in rat liver. Chem. Res. Toxicol., 18, 24-27.
3. de la Asuncion, J.G., Millan, A., Pla, R., Bruseghini, L., Esteras, A., Pallardo, F.V., Sastre, J. and Vina, J. (1996): Mitochondrial glutathione oxidation correlates with age-associated oxidative damage to mitochondrial DNA. FASEB J., 10, 333-338.
4. Fraga, C.G., Shigenaga, M.K., Park, J.W., Degan, P. and Ames, B.N. (1990): Oxidative damage to DNA during aging: 8-hydroxy-2′-deoxyguanosine in rat organ DNA and urine. PNAS, 87, 4533-4537.
5. Griffith, O.W. (1982): Mechanism of action, metabolism, and toxicity of buthionine sulfoximine and its higher homologs, potent inhibitors of glutathione synthesis. J. Biol. Chem., 257, 13704-13712.
6. Hamilton, M.L., Van Remmen, H., Drake, J.A., Yang, H., Guo, Z.M., Kewitt, K., Walter, C.A. and Richardson, A. (2001): Does oxidative damage to DNA increase with age? PNAS, 98, 10469-10474.
7. Hashimoto, K., Takasaki, W., Sato, I. and Tsuda, S. (2007): DNA damage measured by comet assay and 8-OH-dG formation related to blood chemical analyses in aged rats. J. Toxicol. Sci., 32, 249-259.
8. Higami, Y., Shimokawa, I., Okimoto, T. and Ikeda, T. (1994): An age-related increase in the basal level of DNA damage and DNA vulnerability to oxygen radicals in the individual hepatocytes of male F344 rats. Mutat. Res., 316, 59-67.
9. Hirano, T., Yamaguchi, Y. and Kasai, H. (1997): Inhibition of 8-hydroxyguanine repair in testes after administration of cadmium chloride to GSH-depleted rats. Toxicol. Appl. Pharmacol., 147, 9-14.
10. Intano, G.W., Cho, E.J., McMahan, C.A. and Walter, C.A. (2003): Age-related base excision repair activity in mouse brain and liver nuclear extracts. J. Gerontol. A. Biol. Sci. Med. Sci., 58, 205-211.
11. Izzotti, A., Cartiglia, C., Taningher, M., De Flora, S. and Balansky, R. (1999): Age-related increases of 8-hydroxy-2′-deoxyguanos-ine and DNA-protein crosslinks in mouse organs. Mutat. Res., Genet. Toxicol. Environ. Mutagen., 446, 215-223.
12. Kaneko, T., Tahara, S. and Matsuo, M. (1996): Non-linear accumulation of 8-hydroxy-2′-deoxyguanosine, a marker of oxidized DNA damage, during aging. Mutat. Res., 316, 277-285.
13. Kiyosawa, N., Ito, K., Sakuma, K., Niino, N., Kanbori, M., Yamoto, T., Manabe, S. and Matsunuma, N. (2004): Evaluation of glutathione deficiency in rat livers by microarray analysis. Biochem. Pharmacol., 68, 1465-1475.
14. Liu, R. and Choi, J. (2000): Age-associated decline in gamma-glutamylcysteine synthetase gene expression in rats. Free Radic. Biol. Med., 28, 566-574.
15. Maeda, H., Gleiser, C.A., Masoro, E.J., Murata, I., McMahan, C.A. and Yu, B.P. (1985): Nutritional influences on aging of Fischer 344 rats: II. Pathology. J. Gerontol., 40, 671-688.
16. Murawski, U., Kriesten, K. and Egge, H. (1989): Age-related changes of lipid fractions and total fatty acids in the serum of female and male rats aged from 37 to 1200 days. Comp. Biochem. Physiol., Part B: Biochem. Mol. Biol., 94, 525-536.
17. Pallardo, F.V., Asensi, M., Garcia de la Asuncion, J., Anton, V., Lloret, A., Sastre, J. and Vina, J. (1998): Late onset administration of oral antioxidants prevents age-related loss of motor co-ordination and brain mitochondrial DNA damage. Free Radical Res., 29, 617-623.
18. Ruggiero, F.M., Cafagna, F., Gadaleta, M.N. and Quagliariello, E. (1990): Effect of aging and acetyl-L-carnitine on the lipid composition of rat plasma and erythrocytes. Biochem. Biophys. Res. Commun., 170, 621-626.
19. Sai, K., Takagi, A., Umemura, T., Hasegawa, R. and Kurokawa, Y. (1992): Changes of 8-hydroxydeoxyguanosine levels in rat organ DNA during the aging process. J. Environ. Pathol. Toxicol. Oncol., 11, 139-143.
20. Sanz, N., Díez-Fernández, C., Alvarez, A. and Cascales, M. (1997): Age-dependent modifications in rat hepatocyte antioxidant defense systems. J. Hepatol., 27, 525-534.
21. Sasaki, Y.F., Fujikawa, K., Ishida, K., Kawamura, N., Nishikawa, Y., Ohta, S., Satoh, M., Madarame, H., Ueno, S., Susa, N., Matsusaka, N. and Tsuda, S. (1999): The alkaline single cell gel electrophoresis assay with mouse multiple organs: results with 30 aromatic amines evaluated by the IARC and U.S. NTP. Mutat. Res., Genet. Toxicol. Environ. Mutagen., 440, 1-18.
22. Sasaki, Y.F., Tsuda, S., Izumiyama, F. and Nishidate, E. (1997): Detection of chemically induced DNA lesions in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow) using the alkaline single cell gel electrophoresis (Comet) assay. Mutat. Res., Genet. Toxicol. Environ. Mutagen., 388, 33-44.
23. Schmerold, I. and Niedermüller, H. (2001): Levels of 8-hydroxy-2′-deoxyguanosine in cellular DNA from 12 tissues of young and old Sprague-Dawley rats. Exp. Gerontol., 36, 1375-1386.
24. Seo, A.Y., Hofer, T., Sung, B., Judge, S., Chung, H.Y. and Leeuwenburgh, C. (2006): Hepatic oxidative stress during aging: effects of 8% long-term calorie restriction and lifelong exercise. Antioxid. Redox Signaling, 8, 529-538.
25. Sohal, R.S., Agarwal, S., Candas, M., Forster, M.J. and Lal, H. (1994): Effect of age and caloric restriction on DNA oxidative damage in different tissues of C57BL/6 mice. Mech. Ageing Dev., 76, 215-224.
26. Tsuchiya, N., Harada, Y., Taki, M., Minematsu, S., Maemura, S. and Amagaya, S. (1995): [Age-related changes and sex differences on the serum chemistry values in Sprague-Dawley rats - I. 6-30 weeks of age]. Exp. Anim., 43, 671-678.
27. Waner, T., Nyska, A. and Chen, R. (1991): Population distribution profiles of the activities of blood alanine and aspartate aminotransferase in the normal F344 inbred rat by age and sex. Lab. Anim., 25, 263-271.
28. Watanabe, T., Sagisaka, H., Arakawa, S., Shibaya, Y., Watanabe, M., Igarashi, I., Tanaka, K., Totsuka, S., Takasaki, W. and Manabe, S. (2003): A novel model of continuous depletion of glutathione in mice treated with L-buthionine (S,R)-sulfoximine. J. Toxicol. Sci., 28, 455-469.
29. Zhu, Y., Carvey, P.M. and Ling, Z. (2006): Age-related changes in glutathione and glutathione-related enzymes in rat brain. Brain Res., 1090, 35-44.
30. Zubkova, E.V. and Robaire, B. (2004): Effect of glutathione depletion on antioxidant enzymes in the epididymis, seminal vesicles, and liver and on spermatozoa motility in the aging brown Norway rat. Biol. Reprod., 71, 1002-1008.