New strategy for isolating novel nematicidal crystal protein genes from Bacillus thuringiensis strain YBT-1518

Applied and Environmental Microbiology

Volumn 74, Issue 22, 2008, Pages 6997-7001

  Guo, Suxia ^a   Liu, Mei ^a   Peng, Donghai ^a   Ji, Sisi ^a   Wang, Pengxia ^a   Yu, Ziniu ^a   Sun, Ming ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOLOGY; COLLOIDS; CRYSTALLOGRAPHY; DIFFUSERS (OPTICAL); ELECTROPHORESIS; GELATION; NUCLEIC ACIDS; OPTICAL MICROSCOPY; ORGANIC ACIDS; POLYMERS; POWDERS; PROTEINS; SODIUM; SODIUM SULFATE;

BACILLUS THURINGIENSIS; CRY GENES; CRYSTAL PROTEIN GENES; CRYSTAL PROTEINS; DNA LIBRARIES; HOST STRAINS; MICROSCOPIC OBSERVATIONS; NEW STRATEGIES; POLYACRYLAMIDE GEL ELECTROPHORESES; SMALL SIZES; SODIUM DODECYL SULFATES; THURINGIENSIS;

GENES;

BACTERIAL PROTEIN; CRY PROTEIN; DNA; ENDOTOXIN; UNCLASSIFIED DRUG;

BACTERIUM; GENETIC ENGINEERING; NEMATODE; PROTEIN;

ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL GENE; BIOASSAY; BIOLOGICAL PEST CONTROL; CRY GENE; CRY55AA1 GENE; CRY5BA2 GENE; CRY6AA2 GENE; CRYSTAL; LC 50; MICROSCOPY; MOLECULAR CLONING; NEMATODE; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SOUTHERN BLOTTING;

ANIMALS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; CLONING, MOLECULAR; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE LIBRARY; GENES, BACTERIAL; MICROSCOPY; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PLASMIDS; SEQUENCE ANALYSIS, DNA; SURVIVAL ANALYSIS; TYLENCHOIDEA;

BACILLUS THURINGIENSIS; MELOIDOGYNE; MELOIDOGYNE HAPLA; MELOIDOGYNE JAVANICA;

EID: 55949123447     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01346-08     Document Type: Article

References (30)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Andrup, L., J. Damgaard, and K. Wassermann. 1993. Mobilization of small plasmids in Bacillus thuringiensis subsp. israelensis is accompanied by specific aggregation. J. Bacteriol. 175:6530-6536.
3. Andrup, L., G. B. Jensen, A. Wilcks, L. Smidt, L. Hoflack, and J. Mahillon. 2003. The patchwork nature of rolling-circle plasmids: comparison of six plasmids from two distinct Bacillus thuringiensis serotypes. Plasmid 49:205-232.
4. Balasubramanian, P., R. Jayakumar, P. Shambharkar, N. Unnamalai, S. K. Pandian, N. S. Kumaraswami, R. Ilangovan, and V. Sekar. 2002. Cloning and characterization of the crystal protein-encoding gene of Bacillus thuringiensis subsp. yunnanensis. Appl. Environ. Microbiol. 68:408-411.
5. Beron, C. M., L. Curatti, and G. L. Salerno. 2005. New strategy for identification of novel Cry-type genes from Bacillus thuringiensis strains. Appl. Environ. Microbiol. 71:761-765.
6. Ceron, J., A. Ortiz, R. Quintero, L. Guereca, and A. Bravo. 1995. Specific PCR primers directed to identify cryI and cryIII genes within a Bacillus thuringiensis strain collection. Appl. Environ. Microbiol. 61:3826-3831.
7. Crickmore, N., D. R. Zeigler, J. Feitelson, E. Schnepf, J. Van Rie, D. Lereclus, J. Baum, and D. H. Dean. 1998. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62:807-813.
8. Delecluse, A., M. L. Rosso, and A. Ragni. 1995. Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein. Appl. Environ. Microbiol. 61:4230-4235.
9. Gleave, A. P., R. Williams, and R. J. Hedges. 1993. Screening by polymerase chain reaction of Bacillus thuringiensis serotypes for the presence of cryV-like insecticidal protein genes and characterization of a cryV gene cloned from B. thuringiensis subsp. kurstaki. Appl. Environ. Microbiol. 59:1683-1687.
10. Griffitts, J. S., J. L. Whitacre, D. E. Stevens, and R. V. Aroian. 2001. Bt toxin resistance from loss of a putative carbohydrate-modifying enzyme. Science 293:860-864.
11. Jain, D., V. Udayasuriyan, P. I. Arulselvi, S. S. Dev, and P. Sangeetha. 2006. Cloning, characterization, and expression of a new cry2Ab gene from Bacillus thuringiensis strain 14-1. Appl. Biochem. Biotechnol. 128:185-194.
12. Khan, S. A. 2005. Plasmid rolling-circle replication: highlights of two decades of research. Plasmid 53:126-136.
13. Kongsuwan, K., J. Gough, D. Kemp, A. McDevitt, and R. Akhurst. 2005. Characterization of a new Bacillus thuringiensis endotoxin, Cry47Aa, from strains that are toxic to the Australian sheep blowfly, Lucilia cuprina. FEMS Microbiol. Lett. 252:127-136.
14. Kuo, W. S., and K. F. Chak. 1996. Identification of novel cry-type genes from Bacillus thuringiensis strains on the basis of restriction fragment length polymorphism of the PCR-amplified DNA. Appl. Environ. Microbiol. 62:1369-1377.
15. Lee, H. K., and S. S. Gill. 1997. Molecular cloning and characterization of a novel mosquitocidal protein gene from Bacillus thuringiensis subsp. fukuokaensis. Appl. Environ. Microbiol. 63:4664-4670.
16. Li, L., and Z. Yu. 1999. Transformation and expression properties of a Bacillus thuringiensis plasmid-free derivative strain BMB171. Chin. J. Appl. Environ. Biol. 5:395-399.
17. Loeza-Lara, P. D., G. Benintende, J. Cozzi, A. Ochoa-Zarzosa, V. M. Baizabal-Aguirre, J. J. Valdez-Alarcon, and J. E. Lopez-Meza. 2005. The plasmid pBMBt1 from Bacillus thuringiensis subsp. darmstadiensis (INTA Mo14-4) replicates by the rolling-circle mechanism and encodes a novel insecticidal crystal protein-like gene. Plasmid 54:229-240.
18. Luo, M., and R. A. Wing. 2003. An improved method for plant BAC library construction. Methods Mol. Biol. 236:3-20.
19. Masson, L., W. J. Moar, K. van Frankenhuyzen, M. Bosse, and R. Brousseau. 1992. Insecticidal properties of a crystal protein gene product isolated from Bacillus thuringiensis subsp. kenyae. Appl. Environ. Microbiol. 58:642-646.
20. McLinden, J. H., J. R. Sabourin, B. D. Clark, D. R. Gensler, W. E. Workman, and D. H. Dean. 1985. Cloning and expression of an insecticidal k-73 type crystal protein gene from Bacillus thuringiensis var. kurstaki into Escherichia coli. Appl. Environ. Microbiol. 50:623-628.
21. Peng, D., Y. Luo, S. Guo, H. Zeng, S. Ju, Z. Yu, and M. Sun. Elaboration of an electroporation protocol for large plasmids and wild-type strains of Bacillus thuringiensis. J. Appl. Microbiol., in press.
22. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Schnepf, E., N. Crickmore, J. Van Rie, D. Lereclus, J. Baum, J. Feitelson, D. R. Zeigler, and D. H. Dean. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62:775-806.
24. Schnepf, H. E., and H. R. Whiteley. 1981. Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli. Proc. Natl. Acad. Sci. USA 78:2893-2897.
25. Shao, Z., Z. Liu, and Z. Yu. 2001. Effects of the 20-kilodalton helper protein on Cry1Ac production and spore formation in Bacillus thuringiensis. Appl. Environ. Microbiol. 67:5362-5369.
26. Silo-Suh, L. A., B. J. Lethbridge, S. J. Raffel, H. He, J. Clardy, and J. Handelsman. 1994. Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85. Appl. Environ. Microbiol. 60:2023-2030.
27. Song, F., J. Zhang, A. Gu, Y. Wu, L. Han, K. He, Z. Chen, J. Yao, Y. Hu, G. Li, and D. Huang. 2003. Identification of cry1I-type genes from Bacillus thuringiensis strains and characterization of a novel cry1I-type gene. Appl. Environ. Microbiol. 69:5207-5211.
28. Wei, J. Z., K. Hale, L. Carta, E. Platzer, C. Wong, S. C. Fang, and R. V. Aroian. 2003. Bacillus thuringiensis crystal proteins that target nematodes. Proc. Natl. Acad. Sci. USA 100:2760-2765.
29. Widmer, T. L., and G. S. Abawi. 2000. Mechanism of suppression of Meloidogyne hapla and its damage by a green manure of Sudan grass. Plant Dis. 85:562-568.
30. Yu, Z., P. Bai, W. Ye, F. Zhang, L. Ruan, Z. Yu, and M. Sun. 2008. A novel negative regulatory factor for nematicidal Cry protein gene expression in Bacillus thuringiensis. J. Microbiol. Biotechnol. 18:1033-1039.