Novel application of 4-nitro-7-(1-piperazinyl)-2,1,3-benzoxadiazole to visualize lysosomes in live cells

BioTechniques

Volumn 45, Issue 4, 2008, Pages 465-468

  Ishiguro, Kazuhiro ^a   Ando, Takafumi ^b   Goto, Hidemi ^b  

^a NAGOYA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b NAGOYA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

4 NITRO 7 (1 PIPERAZINYL) 1,2,3 BENZOXADIAZOLE; FLUORESCENT DYE; UNCLASSIFIED DRUG; 4 NITRO 7 PIPERAZINO 2,1,3 BENZOXADIAZOLE; 4-NITRO-7-PIPERAZINO-2,1,3-BENZOXADIAZOLE; OXADIAZOLE DERIVATIVE; PIPERAZINE DERIVATIVE;

ANIMAL CELL; ARTICLE; CHEMICAL STRUCTURE; CONTROLLED STUDY; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; IMAGE ANALYSIS; LYSOSOME; MOLECULAR PROBE; NONHUMAN; PH; SOLVENT EFFECT; SPECTROSCOPY; ANIMAL; CELL LINE; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; ECONOMICS; METABOLISM; MOUSE; RAT;

ANIMALS; CELL LINE; CELL SURVIVAL; CYTOLOGICAL TECHNIQUES; HUMANS; LYSOSOMES; MICE; MICROSCOPY, FLUORESCENCE; OXADIAZOLES; PIPERAZINES; RATS;

EID: 55949098539     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112912     Document Type: Article

References (10)

1. Ghosh, P.B. and M.W. Whitehouse. 1968. 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole: a new fluorigenic reagent for amino acids and other amines. Biochem. J. 108:155-156.
2. Toyo'oka, T., M. Ishibashi, Y. Takeda, K. Nakashima, S. Akiyama, S. Uzu, and K. Imai. 1991. Precolumn fluorescence tagging reagent for carboxylic acids in high-performance liquid chromatography: 4-substituted-7-aminoalkylamino-2,1,3-benzoxadiazoles. J. Chromatogr. 588:61-71.
3. Santa, T., D. Matsumura, C. Huang, C. Kitada, and K. Imai. 2002. Design and synthesis of a hydrophilic fluorescent derivatization reagent for carboxylic acids, 4-N-(4-N-aminoethyl)piperazino-7-nitro-2,1,3-benzoxadiazole (NBD-PZ-NH2), and its application to capillary electrophoresis with laser-induced fluorescence detection. Biomed. Chromatogr. 16:523-528.
4. Luzio, J.P., P.R. Pryor, and N.A. Bright. 2007. Lysosomes: fusion and function. Nat. Rev. Mol. Cell Biol. 8:622-632.
5. Kaufmann, A.M. and J.P. Krise. 2007. Lysosomal sequestration of amine-containing drugs: analysis and therapeutic implications. J. Pharm. Sci. 96:729-746.
6. Bowman, E.J., A. Siebers, and K. Altendorf. 1988. Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc. Natl. Acad. Sci. USA 85:7972-7976.
7. Fehrenbacher, N. and M. Jaattela. 2005. Lysosomes as targets for cancer therapy. Cancer Res. 65:2993-2995.
8. Kroemer, G. and M. Jaattela. 2005. Lysosomes and autophagy in cell death control. Nat. Rev. Cancer 5:886-897.
9. Simon, S., D. Roy, and M. Schindler. 1994. Intracellular pH and the control of multidrug resistance. Proc. Natl. Acad. Sci. USA 91:1128-1132.
10. Warburg, O. 1956. On the origin of cancer cells. Science 123:309-314.