Function of the Bacillus subtilis transcription elongation factor NusG in hairpin-dependent RNA polymerase pausing in the trp leader

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 42, 2008, Pages 16131-16136

  Yakhnin, Alexander V ^a   Yakhnin, Helen ^a   Babitzke, Paul ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

NusA; Transcription attenuation; Translational control

Indexed keywords

RNA POLYMERASE; TRANSCRIPTION ELONGATION FACTOR; TRANSCRIPTION ELONGATION FACTOR NUSA; TRANSCRIPTION ELONGATION FACTOR NUSG; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; ESCHERICHIA COLI; GENE; GENE OVEREXPRESSION; NONHUMAN; NUSA GENE; NUSG GENE; OPERON; PLASMID; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION;

BACILLUS SUBTILIS; BASE SEQUENCE; DNA-DIRECTED RNA POLYMERASES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PEPTIDE ELONGATION FACTORS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; TRYPTOPHAN;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 55849112295     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0808842105     Document Type: Article

References (18)

1. Borukhov S, Nudler E (2008) RNA polymerase: The vehicle of transcription. Trends Microbiol 16:126-134.
2. Squires CL, Zaporojets D (2000) Proteins shared by the transcription and translation machines. Annu Rev Microbiol 54:775-798.
3. Cardinale CJ, et al. (2008) Termination factor Rho and its cofactors NusA and NusG silence foreign DNA in E. coli. Science 320:935-938.
4. Schmidt MC, Chamberlin MJ (1987) nusA protein of Escherichia coli is an efficient transcription termination factor for certain terminator sites. J Mol Biol 195:809-818.
5. Artsimovitch I, Landick R (2000) Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals. Proc Natl Acad Sci USA 97:7090-7095.
6. Chalissery J, Banerjee S, Bandey I, Sen R (2007) Transcription termination-defective mutants of Rho: Role of different functions of Rho in releasing RNA from the elongation complex. J Mol Biol 371:855-872.
7. Gollnick P, Babitzke P, Antson A, Yanofsky C (2005) Complexity in regulation of tryptophan biosynthesis in Bacillus subtilis. Annu Rev Genet 39:47-68.
8. McGraw A, Bevilacqua PC, Babitzke P (2007) TRAP-5′ stem-loop interaction increases the efficiency of transcription termination in the Bacillus subtilis trpEDCFBA operon leader region. RNA 13:2020-2033.
9. Yakhnin AV, Babitzke P (2002) NusA-stimulated RNA polymerase pausing and termination participates in the Bacillus subtilis trp operon attenuation mechanism in vitro. Proc Natl Acad Sci USA 99:11067-11072.
10. Yakhnin AV, Yakhnin H, Babitzke P (2006) RNA polymerase pausing regulates translation initiation by providing additional time for TRAP-RNA interaction. Mol Cell 24:547-557.
11. Artsimovitch I, Svetlov V, Anthony L, Burgess RR, Landick R (2000) RNA polymerases from Bacillus subtilis and Escherichia coli differ in recognition of regulatory signals in vitro. J Bacteriol 182:6027-6035.
12. Toulokhonov I, Artsimovitch I, Landick R (2001) Allosteric control of RNA polymerase by a site that contacts nascent RNA hairpins. Science 292:730-733.
13. Toulokhonov I, Zhang J, Palangat M, Landick R (2007) A central role of the RNA polymerase trigger loop in active-site rearrangement during transcriptional pausing. Mol Cell 27:406-419.
14. Epshtein V, Nudler E (2003) Cooperation between RNA polymerase molecules in transcription elongation. Science 300:801-805.
15. Bar-Nahum G, et al. (2005) A ratchet mechanism of transcription elongation and its control. Cell 120:183-193.
16. Belogurov GA, et al. (2007) Structural basis for converting a general transcription factor into an operon-specific virulence regulator. Mol Cell 26:117-129.
17. 70 in promoter-proximal pausing. Cell 86:485-493.
18. Doherty GP, Meredith DH, Lewis PJ (2006) Subcellular partitioning of transcription factors in Bacillus subtilis. J Bacteriol 188:4101-4110.