Comparison between the standardized Clinical and Laboratory Standards Institute M38-A2 method and a 2,3-bis(2-methoxy-4-nitro-5-[(sulphenylamino) carbonyl]-2H-tetrazolium hydroxide-based method for testing antifungal susceptibility of dermatophytes

Journal of Clinical Microbiology

Volumn 46, Issue 11, 2008, Pages 3668-3671

  Shehata, Atef S ^a,b   Mukherjee, Pranab K ^b   Ghannoum, Mahmoud A ^b  

^a Suez Canal University   (Egypt)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2,3 BIS(2 METHOXY 4 NITRO 5 [(SULPHENYLAMINO)CARBONYL] 2H TETRAZOLIUM HYDROXIDE; CICLOPIROX; HYDROXIDE; TERBINAFINE; UNCLASSIFIED DRUG; VORICONAZOLE;

ANTIFUNGAL SUSCEPTIBILITY; ARTICLE; CONTROLLED STUDY; DERMATOPHYTE; EPIDERMOPHYTON FLOCCOSUM; FUNGUS ISOLATION; INTERMETHOD COMPARISON; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; QUALITY CONTROL; TRICHOPHYTON MENTAGROPHYTES; TRICHOPHYTON RUBRUM; TRICHOPHYTON TONSURANS;

ANTIFUNGAL AGENTS; ARTHRODERMATACEAE; HUMANS; INDICATORS AND REAGENTS; MICROBIAL SENSITIVITY TESTS; MICROBIAL VIABILITY; MYCOLOGY; NAPHTHALENES; PYRIDONES; PYRIMIDINES; SENSITIVITY AND SPECIFICITY; SPECTROPHOTOMETRY; TETRAZOLIUM SALTS; TRIAZOLES; VITAMIN K 3;

ARTHRODERMA BENHAMIAE; ARTHRODERMATACEAE; EPIDERMOPHYTON FLOCCOSUM; TRICHOPHYTON RUBRUM; TRICHOPHYTON TONSURANS;

EID: 55849099562     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01242-08     Document Type: Article

References (17)

1. Altman, F. P. 1976. Tetrazolium salts and formazans. Prog. Histochem. Cytochem. 9:1-56.
2. Antachopoulos, C., J. Meletiadis, E. Roilides, T. Sein, and T. J. Walsh. 2006. Rapid susceptibility testing of medically important zygomycetes by XTT assay. J. Clin. Microbiol. 44:553-560.
3. Antachopoulos, C., J. Meletiadis, T. Sein, E. Roilides, and T. J. Walsh. 2007. Use of high inoculum for early metabolic signalling and rapid susceptibility testing of Aspergillus species. J. Antimicrob. Chemother. 59:230-237.
4. Berg, J. C., K. L. Hamacher, and G. D. Roberts. 2007. Pseudomycetoma caused by Microsporum canis in an immunosuppressed patient: a case report and review of the literature. J. Cutan. Pathol. 34:431-434.
5. Berridge, M. V., P. M. Herst, and A. S. Tan. 2005. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol. Annu. Rev. 11:127-152.
6. CLSI. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard, 2nd ed. CLSI document M38-A2. Clinical and Laboratory Standards Institute, Wayne, PA.
7. Hawser, S. P., C. Jessup, J. Vitullo, and M. A. Ghannoum. 2001. Utility of 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyl-amino)carbonyl]- 2H-tetrazolium hydroxide (XTT) and minimum effective concentration assays in the determination of antifungal susceptibility of Aspergillus fumigatus to the lipopeptide class compounds. J. Clin. Microbiol. 39:2738-2741.
8. Hawser, S. P., H. Norris, C. J. Jessup, and M. A. Ghannoum. 1998. Comparison of a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) colorimetric method with the standardized National Committee for Clinical Laboratory Standards method of testing clinical yeast isolates for susceptibility to antifungal agents. J. Clin. Microbiol. 36:1450-1452.
9. Knight, S. A., and A. Dancis. 2006. Reduction of 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT) is dependent on CaFRE10 ferric reductase for Candida albicans grown in unbuffered media. Microbiology 152:2301-2308.
10. Marr, K. A., T. R. Rustad, J. H. Rex, and T. C. White. 1999. The trailing end point phenotype in antifungal susceptibility testing is pH dependent. Antimicrob. Agents Chemother. 43:1383-1386.
11. Meletiadis, J., J. W. Mouton, J. F. Meis, B. A. Bouman, J. P. Donnelly, and P. E. Verweij. 2001. Colorimetric assay for antifungal susceptibility testing of Aspergillus species. J. Clin. Microbiol. 39:3402-3408.
12. Meletiadis, J., J. W. Mouton, J. F. Meis, B. A. Bouman, P. J. Donnelly, and P. E. Verweij. 2001. Comparison of spectrophotometric and visual readings of NCCLS method and evaluation of a colorimetric method based on reduction of a soluble tetrazolium salt, 2,3-bis[2-methoxy-4-nitro-5- [(sulfenylamino) carbonyl]-2H-tetrazolium-hydroxide], for antifungal susceptibility testing of Aspergillus species. J. Clin. Microbiol. 39:4256-4263.
13. Mowat, E., J. Butcher, S. Lang, C. Williams, and G. Ramage. 2007. Development of a simple model for studying the effects of antifungal agents on multicellular communities of Aspergillus fumigatus. J. Med. Microbiol. 56: 1205-1212.
14. Nir-Paz, R., H. Elinav, G. E. Pierard, D. Walker, A. Maly, M. Shapiro, R. C. Barton, and I. Polacheck. 2003. Deep infection by Trichophyton rubrum in an immunocompromised patient. J. Clin. Microbiol. 41:5298-5301.
15. Revankar, S. G., W. R. Kirkpatrick, R. K. McAtee, A. W. Fothergill, S. W. Redding, M. G. Rinaldi, and T. F. Patterson. 1998. Interpretation of trailing endpoints in antifungal susceptibility testing by the National Committee for Clinical Laboratory Standards method. J. Clin. Microbiol. 36:153-156.
16. Smith, S. L., and M. J. Stiller. 1997. Fungal infections, p. 100-105. In K. A. Arndt, B. U. Wintroub, J. K. Robinson, and P. E. Leboit (ed.), Primary care dermatology, vol. 22. W.B. Saunders Company, Philadelphia, PA.
17. Weitzman, I., and R. C. Summerbell. 1995. The dermatophytes. Clin. Microbiol. Rev. 8:240-259.