Probing conformational changes of prestin with thiol-reactive optical switches

Biophysical Journal

Volumn 95, Issue 6, 2008, Pages 3036-3042

  Fang, Jie ^a   Sakata, Tomoyo ^b   Marriott, Gerard ^b   Iwasa, Kuni H ^a  

^a NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANION TRANSPORT PROTEIN; BIOTIN; CYSTEINE; DRUG DERIVATIVE; IODOACETAMIDE; MALEIMIDE; MALEIMIDE DERIVATIVE; THIOL DERIVATIVE;

ANIMAL; ARTICLE; CELL MEMBRANE; CHEMISTRY; DRUG EFFECT; ELECTRIC CAPACITANCE; GENETIC TRANSFECTION; GUINEA PIG; HAIR CELL; HUMAN; METABOLISM; OPTICS; PROTEIN CONFORMATION;

ANIMALS; ANION TRANSPORT PROTEINS; BIOTIN; CELL MEMBRANE; CYSTEINE; ELECTRIC CAPACITANCE; GUINEA PIGS; HAIR CELLS, AUDITORY, OUTER; HUMANS; IODOACETAMIDE; MALEIMIDES; OPTICS; PROTEIN CONFORMATION; SULFHYDRYL COMPOUNDS; TRANSFECTION;

EID: 55549085898     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1529/biophysj.108.132878     Document Type: Article

References (25)

1. Liberman, M. C., J. Gao, D. Z. He, X. Wu, S. Jia, and J. Zuo. 2002. Prestin is required for electromotility of the outer hair cell and for the cochlear amplifier. Nature. 419:300-304.
2. Iwasa, K. H. 1993. Effect of stress on the membrane capacitance of the auditory outer hair cell. Biophys. J. 65:492-498.
3. Dallos, P., R. Hallworth, and B. N. Evans. 1993. Theory of electrically driven shape changes of cochlear outer hair cells. J. Neurophysiol. 70:299-323.
4. Mountain, D. C., and A. E. Hubbard. 1994. A piezoelectric model of outer hair cell function. J. Acoust. Soc. Am. 95:350-354.
5. Gale, J. E., and J. F. Ashmore. 1994. Charge displacement induced by rapid stretch in the basolateral membrane of the guinea-pig outer hair cell. Proc. Roy. Soc. (Lond.) B Biol. Sci. 255:233-249.
6. Tolomeo, J. A., and C. R. Steele. 1995. Orthotropic piezoelectric properties of the cochlear outer hair cell wall. J. Acoust. Soc. Am. 97:3006-3011.
7. Iwasa, K. H. 1994. A membrane model for the fast motility of the outer hair cell. J. Acoust. Soc. Am. 96:2216-2224.
8. Adachi, M., and K. H. Iwasa. 1999. Electrically driven motor in the outer hair cell: Effect of a mechanical constraint. Proc. Natl. Acad. Sci. USA. 96:7244-7249.
9. Ashmore, J. F. 1990. Forward and reverse transduction in guinea-pig outer hair cells: the cellular basis of the cochlear amplifier. Neurosci. Res. Suppl. 12:S39-S50.
10. Santos-Sacchi, J. 1991. Reversible inhibition of voltage-dependent outer hair cell motility and capacitance. J. Neurophysiol. 11:3096-3110.
11. Zheng, J., W. Shen, D. Z.-Z. He, K. B. Long, L. D. Madison, and P. Dallos. 2000. Prestin is the motor protein of cochlear outer hair cells. Nature. 405:149-155.
12. Sakata, T., Y. Yan, and G. Marriott. 2005. Optical switching of dipolar interactions on proteins. Proc. Natl. Acad. Sci. USA. 102:4759-4764.
13. Dong, X. X., M. Ospeck, and K. H. Iwasa. 2002. Piezoelectric reciprocal relationship of the membrane motor in the cochlear outer hair cell. Biophys. J. 82:1254-1259.
14. Dong, X.-X., and K. H. Iwasa. 2004. Tension sensitivity of prestin: comparison with the membrane motor in outer hair cells. Biophys. J. 86:1201-1208.
15. Kalinec, F., and B. Kachar. 1993. Inhibition of outer hair cell electromotility by sulfhydryl specific reagents. Neurosci. Lett. 157:231-234.
16. Adachi, M., and K. H. Iwasa. 1997. Effect of diamide on force production and axial stiffness of the cochlear outer hair cell. Biophys. J. 73:2809-2818.
17. Santos-Sacchi, J., and M. Wu. 2004. Protein- and lipid-reactive agents alter outer hair cell lateral membrane motor charge movement. J. Membr. Biol. 200:83-92.
18. Fang, J., and K. H. Iwasa. 2007. Effects of chlorpromazine and trinitrophenol on the membrane motor of outer hair cells. Biophys. J. 93:1809-1817.
19. Iwasa, K. H. 2001. A two-state piezoelectric model for outer hair cell motility. Biophys. J. 81:2495-2506.
20. Oliver, D., and B. Fakler. 1999. Expression density and functional characteristics of the outer hair cell motor protein are regulated during postnatal development in rat. J. Physiol. 519:791-800.
21. Dallos, P., and B. Fakler. 2002. Prestin, a new type of motor protein. Nat. Rev. Mol. Cell Biol. 3:104-111.
22. Muallem, D., and J. F. Ashmore. 2006. An anion antiporter model of prestin, the outer hair cell motor protein. Biophys. J. 90:4035-4045.
23. Santos-Sacchi, J., and H.-B. Zhao. 2003. Excitation of fluorescent dyes inactivates the outer hair cell integral membrane motor protein prestin and betrays its lateral mobility. Pflugers Arch. 446:617-622.
24. Navaratnam, D., J.-P. Bai, H. Samaranayake, and J. Santos-Sacchi. 2005. N-terminal-mediated homomultimerization of prestin, the outer hair cell motor protein. Biophys. J. 89:3345-3352.
25. Déak, L., J. Zheng, A. Orem, G. G. Du, S. Aguinaga, K. Matsuda, and P. Dallos. 2005. Effects of cyclic nucleotides on the function of prestin. J. Physiol. 563:483-496.