Cell-free expression and functionality analysis of the tobacco lectin

In Vitro Cellular and Developmental Biology - Animal

Volumn 44, Issue 7, 2008, Pages 228-235

  Vandenborre, Gianni ^a   Lannoo, Nausicaä ^a   Smagghe, Guy ^a   Daniel, Erica ^b,c   Breite, Andrew ^b,d   Soin, Thomas ^a   Jacobsen, Linda ^b,e   Van Damme, Els J M ^a  

^a GHENT UNIVERSITY   (Belgium)

^b Roche Diagnostics Corporation   (United States)

^c INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d VitaCyte LLC   (United States)

^e Berit Biotech LLC   (United States)

Author keywords

Escherichia coli; Expression; Lectin; Nictaba; RTS

Indexed keywords

LECTIN; RECOMBINANT PROTEIN;

ARTICLE; CELL LYSATE; CODON USAGE; ESCHERICHIA COLI; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TOBACCO; ANIMAL; ANTIGEN ANTIBODY REACTION; CARBOHYDRATE METABOLISM; CELL FREE SYSTEM; CELL LINE; CELL PROLIFERATION; CHEMISTRY; CYTOLOGY; DRUG EFFECT; GASTROINTESTINAL TRACT; INSECT; MASS SPECTROMETRY; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SYNTHESIS; WESTERN BLOTTING;

ESCHERICHIA COLI; HEXAPODA; NICOTIANA TABACUM;

AGGLUTINATION; ANIMALS; BLOTTING, WESTERN; CARBOHYDRATE METABOLISM; CELL LINE; CELL PROLIFERATION; CELL-FREE SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GASTROINTESTINAL TRACT; INSECTS; LECTINS; MASS SPECTROMETRY; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; TOBACCO;

EID: 55249106248     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11626-008-9123-z     Document Type: Article

References (15)

1. M. V. Berridge A. S. Tan 1993 Characterization of the cellular reduction of 3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction Arch. Biochem. Biophys. 2 474 482
2. J. M. Betton 2003 Rapid translation system (RTS): a promising alternative for recombinant protein production Curr. Protein Pept. Sci. 4 73 80
3. Y. Chen W. J. Peumans B. Hause J. Bras M. Kumar P. Proost 2002 Jasmonic acid methyl ester induces the synthesis of a cytoplasmic/nuclear chitooligosaccharide-binding lectin in tobacco leaves FASEB J. 16 905 907
4. R. S. Hakim M. B. Blackburn P. Corti D. B. Gelman C. Goodman K. Elsen 2007 Growth and mitogenic effects of arylphorin in vivo and in vitro Arch. Insect Biochem. Physiol. 64 63 73
5. M. Hoffmann C. Nemetz K. Madin B. Buchberger 2004 Rapid translation system: a novel cell-free way from gene to protein Biotechnol. Annu. Rev. 10 1 30
6. U. K. Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685
7. N. Lannoo W. J. Peumans E. J. M. Van Damme 2006 The presence of jasmonate-inducible lectin genes in some but not all Nicotiana species explains a marked intragenus difference in plant responses to hormone treatment J. Exp. Bot. 57 3145 3155
8. N. Lannoo W. J. Peumans E. Van Pamel R. Alvarez T. C. Xiong G. Hause 2006 Localization and in vitro binding studies suggest that the cytoplasmic/nuclear tobacco lectin can interact in situ with high-mannose and complex N-glycans FEBS Lett. 580 6329 6337
9. N. Lannoo G. Vandenborre O. Miersch G. Smagghe C. Wasternack W. J. Peumans 2007 The jasmonate-induced expression of the Nicotiana tabacum leaf lectin Plant Cell Physiol. 48 1207 1218
10. W. J. Peumans A. Barre Q. Hao P. Rougé E. J. M. Van Damme 2000 Higher plants developed structurally different motifs to recognize foreign glycans Trends Glycosci. Glyco. 12 83 101
11. W. J. Peumans E. J. M. Van Damme 1995 Lectins as plant defense proteins Plant Physiol. 109 347 352
12. E. J. M. Van Damme A. Barre P. Rougé W. J. Peumans 2004 Cytoplasmic/nuclear plant lectins: a new story Trends Plant Sci. 9 484 489
13. E. J. M. Van Damme N. Lannoo E. Fouquaert W. J. Peumans 2003 The identification of inducible cytoplasmic/nuclear carbohydrate-binding proteins urges to develop novel concepts about the role of plant lectins Glycoconj. J. 20 449 460
14. E. J. M. Van Damme W. J. Peumans A. Barre P. Rougé 1998 Plant lectins: a composite of several distinct families of structurally and evolutionary related proteins with diverse biological roles Crit. Rev. Plant Sci. 17 575 692
15. Van Damme E. J. M.; Rougé P.; Peumans W. J. Carbohydrate-protein interactions: plant lectins. In: KamerlingJ. P.; BoonsG. J.; LeeY. C.; SuzukiA.; TaniguchiN.; VoragenA. G. J. (eds) Comprehensive glycoscience-from chemistry to systems biology. Elsevier, New York, pp 563-599; 2007.