Immunogold staining of London resin (LR) white sections for transmissions electron (TEM)

Cold Spring Harbor Protocols

Volumn 3, Issue 6, 2008, Pages

  Skepper, Jeremy N ^a   Powell, Janet M ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ACRYLIC ACID RESIN; ALCOHOL; ALDEHYDE; EPOXY RESIN; LEAD; RESIN; URANYL ACETATE;

ARTICLE; CELL ULTRASTRUCTURE; CROSS LINKING; EMBEDDING; IMMOBILIZED CELL; IMMUNOCHEMISTRY; IMMUNOGOLD STAINING; POLYMERIZATION; TISSUE FIXATION; TRANSMISSION ELECTRON MICROSCOPY; VISCOSITY;

EID: 55249093387     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5016     Document Type: Article

References (9)

1. Berryman, M.A. and Rodewald, R.D. 1990. An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes. J. Histochem. Cytochem. 38: 159-170.
2. Gibbons, I.R. and Grimstone, A.V. 1960. On flagellar structure in certain flagellates. J. Biophys. Biochem. Cytol. 7: 697-716.
3. Griffiths, G., Burke, B., and Lucocq, J. 1993, Fine structure immunocytochemistry.
4. Reynolds, E.S. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17: 208-212.
5. Skepper, J.N. 2000. Immunocytochemical strategies for electron microscopy: Choice or compromise. J. Microsc. 199: 1-36.
6. Skepper, J.N. and Powell, J.M. 2008a. Ultrastructural immunochemistry. CSH Protocols (this issue) doi: 10.1101/pdb.top47.
7. Skepper, J.N. and Powell, J.M. 2008b. Immunogold staining of epoxy resin sections for transmission electron microscopy (TEM). CSH Protocols (this issue) doi: 10.1101/pdb.prot5015.
8. Skepper, J.N. and Powell, J.M. 2008c. Immunogold staining following freeze substitution and low temperature embedding after chemical fixation or after cryoimmobilization for transmission electron microscopy (TEM). CSH Protocols (this issue) doi: 10.1101/ pdb.prot5017.
9. Skepper, J.N. and Powell, J.M. 2008d. Immunogold staining of ultrathin thawed cryosections for transmission electron microscopy (TEM). CSH Protocols (this issue) doi: 10.1101/pdb.prot5018.