Isolation and whole-cell patch clamping of Aribidopsis guard cell protoplasts

Cold Spring Harbor Protocols

Volumn 3, Issue 6, 2008, Pages

  Zhang, Wei ^a   Nilson, Sarah E ^a   Assmann, Sarah M ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CELLULASE; POLYGALACTURONASE; POTASSIUM ION;

ARABIDOPSIS; ARTICLE; GUARD CELL; MICROELECTRODE; NONHUMAN; PATCH CLAMP; PLANT CELL; PLANT PHYSIOLOGY; POTASSIUM CURRENT; PROTOPLAST ISOLATION;

EID: 55249092460     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5014     Document Type: Article

References (33)

1. Assmann, S.M. and Romano, L. 1999. Secondary messenger regulation of ion channels/plant patch clamping. Methods Enzymol. 294: 410-441.
2. Assmann, S.M., Simoncini, L., and Schroeder, J.I. 1985. Blue light activates electrogenic ion pumping in guard cell protoplasts of Vicia faba. Nature 318: 285-287.
3. 2+-calmodulin modulates ion channel activity in storage protein vacuoles of barley aleurone cells. Plant Cell 6: 277-285.
4. Bouteau, F., Perino, C., Cornel, D., and Rona, J.P. 1993. Sugar absorption and potassium channels in protoplasts of Hevea brasiliensis laticiferous vessels. Bioelectrochem. Bioenerg 31: 215-228.
5. 2+, annexins, and GTP modulate exocytosis from maize root cap protoplasts. Plant Cell 10: 1267-1276.
6. Cho, M.H. and Spalding, E.P. 1996. An anion channel in Arabidopsis hypocotyls activated by blue light. Proc. Natl. Acad. Sci. 93: 8134-8138.
7. Coursol, S., Fan, L., Le Stunff, H., Spiegel, S., Gilroy, S., and Assmann, S.M. 2003. Sphingolipid signalling in Arabidopsis guard cells involves heterotrimeric G proteins. Nature 423: 651-654.
8. Elzenga, J.T.M. and Van Volkenburgh, E. 1997. Characterization of a light-controlled anion channel in the plasma membrane of mesophyll cells of pea. Plant Physiol. 113: 1419-1426.
9. Elzenga, J.T., Keller, C.P., and Van Volkenburgh, E. 1991. Patch clamping protoplasts from vascular plants: Method for the quick isolation of protoplasts having a high success rate of gigaseal formation. Plant Physiol. 97: 1573-1575.
10. + current across the plasma membrane of guard cells from a grass: Zea mays. Planta 186: 282-293.
11. Falke, L.C., Edwards, K.L., Pickard, B.G., and Misler, S. 1988. A stretch-activated anion channel in tobacco protoplasts. FEBS Lett 237: 141-144.
12. + channel in the plasma membrane of Brassica pollen protoplasts. Plant Cell Physiol. 40: 859-865.
13. + uptake and membrane potential control). Plant Physiol. 105: 1399-1408.
14. + currents across the plasma membrane of pollen grain and tube protoplasts of Lilium longiflorum. J. Membr. Biol 193: 99-108.
15. + channel profile and electrical properties of Arabidopsis root hairs. FEBS Lett 508: 463-469.
16. Jacobsen, J.V., Zwar, J.A., and Chandler, P.M. 1985. Gibberellic-acid-responsive protoplasts from mature aleurone of Himalaya barley. Planta 163: 430-438.
17. Kruse, T., Tallman, G., and Zeiger, E. 1989. Isolation of guard cell protoplasts from mechanically prepared epidermis of Vicia faba leaves. Plant Physiol. 90: 1382-1386.
18. Leonhardt, N., Kwak, J.M., Robert, N., Waner, D., Leonhardt, G., and Schroeder, J.I. 2004. Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive abscisic acid hypersensitive protein phosphatase 2C mutant. Plant Cell 16: 596-615.
19. + current in mesophyll cells of Vicia faba L. Proc. Natl. Acad. Sci. 90: 262-266.
20. Li, J., Wang, X.Q., Watson, M.B., and Assmann, S.M. 2000. Regulation of abscisic acid-induced stomatal closure and anion channels by guard cell AAPK kinase. Science 287: 300-303.
21. Pandey, S., Wang, X-Q., Coursol, S.A., and Assmann, S.M. 2002. Preparation and applications of Arabidopsis thaliana guard cell protoplasts. New Phytol 153: 517-526.
22. Pei, Z.M., Kuchitsu, K., Ward, J.M., Schwarz, M., and Schroeder, J.I. 1997. Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants. Plant Cell 9: 409-423.
23. +-selective currents in the plasma membrane of protoplasts from maize root cortex and stele. Plant J. 8: 811-825.
24. + channels in mesophyll cells of Arabidopsis thaliana. Plant Cell Physiol 39: 1133-1144.
25. + selectivity of a cation channel in the plasma membrane of root cells does not differ in salt-tolerant and salt-sensitive wheat species. Plant Physiol. 97: 598-605.
26. Schroeder, J.I., Hedrich, R., and Fernandez, J.M. 1984. Potassium-selective single channels in guard-cell protoplasts of Vicia faba. Nature 312: 361-362.
27. Stoeckel, H. and Takeda, K. 1993. Plasmalemmal, voltage-dependent ionic currents from excitable pulvinar motor cells of Mimosa pudica. J. Membr. Biol 131: 179-192.
28. Wang, X.Q., Ullah, H., Jones, A.M., and Assmann, S.M. 2001. G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells. Science 292: 2070-2072.
29. 2+-permeable channels in the plasma membrane of Arabidopsis pollen are regulated by actin microfilaments. Plant Physiol. 136: 3892-3904.
30. Wegner, L.H. and Raschke, K. 1994. Ion channels in the xylem parenchyma of barley roots: A procedure to isolate protoplasts from this tissue and a patch-clamp exploration of salt passageways into xylem vessels. Plant Physiol. 105: 799-813.
31. + channel. Proc. Natl. Acad. Sci. 91: 6310-6314.
32. + channel activation in Vicia guard cells? Plant Physiol. 107: 101-109.
33. Zhang, W., Fan, L.M., and Wu, W.H. 2007. Osmo-sensitive and stretch-activated calcium-permeable channels in Vicia faba guard cells are regulated by actin dynamics. Plant Physiol. 143: 1140-1151