A MALDI TOF MS-based minisequencing method for rapid detection of TEM-type extended-spectrum beta-lactamases in clinical strains of Enterobacteriaceae

Journal of Microbiological Methods

Volumn 75, Issue 3, 2008, Pages 385-391

  Ikryannikova, L N ^a   Shitikov, E A ^a   Zhivankova, D G ^a   Il'ina, E N ^a   Edelstein, M V ^b   Govorun, V M ^a  

^a N N BLOKHIN NATIONAL MEDICAL RESEARCH CENTER OF ONCOLOGY   (Russian Federation)

^b Institute of Antimicrobial Chemotherapy   (Russian Federation)

Author keywords

Enterobacteriaceae; MALDI TOF mass spectrometry; Minisequencing; TEM beta lactamase

Indexed keywords

EXTENDED SPECTRUM BETA LACTAMASE;

ARTICLE; BLA TEM GENE; CODON; CONTROLLED STUDY; ENTEROBACTERIACEAE; ESCHERICHIA COLI; GENE; GENE SEQUENCE; KLEBSIELLA PNEUMONIAE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; SINGLE NUCLEOTIDE POLYMORPHISM;

BACTERIAL PROTEINS; BASE SEQUENCE; BETA-LACTAMASES; DRUG RESISTANCE, BACTERIAL; ENTEROBACTERIACEAE; ENTEROBACTERIACEAE INFECTIONS; ESCHERICHIA COLI; HUMANS; KLEBSIELLA PNEUMONIAE; MOLECULAR SEQUENCE DATA; POLYMORPHISM, SINGLE NUCLEOTIDE; SEQUENCE ANALYSIS, DNA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ENTEROBACTERIACEAE; ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE;

EID: 55149116096     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.07.005     Document Type: Article

References (17)

1. Babic M., Hujer A.M., and Bonomo R.A. What's new in antibiotic resistance? Focus on beta-lactamases. Drug Resist. Updat. 9 (2006) 142-156
2. Blondal Th., Waage B.G., Smarason S.V., Jonsson F., Fjalldal S.B., Stefansson K., Gulcher J., and Smith A.V. A novel MALDI-TOF based methodology for genotyping single nucleotide polymorphisms. Nucleic Acids Res. 31 (2003) e155
3. Bradford P.A. Extended-spectrum b-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin. Microbiol. Rev. 14 (2001) 933-951
4. Edelstein, M., Suvorov, M., Edelstein, I., Kozlov, R., 2000. Identification of the naturally occurring variant genes blaTEM-1d and blaTEM-70 encoding broad-spectrum TEM-type beta-lactamases. 10th European Congress of Clinical Microbiology and Infectious Disease. Book of Abstracts P278:14/1.
5. Fiett J., Palucha A., Miaczynska B., Stankiewicz M., Przondo-Mordarska H., Hryniewicz W., and Gniadkowski M. A novel complex mutant beta-lactamase, TEM-68, identified in a Klebsiella pneumoniae isolate from an outbreak of extended-spectrum beta-lactamase-producing Klebsiellae. Antimicrob. Agents Chemother. 44 (2000) 1499-1505
6. Gniadkowski M. Evolution and epidemiology of extended-spectrum b-lactamases (ESBLs) and ESBL-producing microorganisms. Clin Microbiol Infect 7 (2001) 597-608
7. Gootz T.D. The forgotten Gram-negative bacilli: what genetic determinants are telling us about the spread of antibiotic resistance. Biochem. Pharmacol. 71 (2006) 1073-1084
8. Grimm V., Ezaki S., Susa M., Knabbe C., Schmid R.D., and Bachmann T.T. Use of DNA microarrays for rapid genotyping of TEM beta-lactamases that confer resistance. J. Clin. Microbiol. 42 (2004) 3766-3774
9. Haff L.A., and Smirnov I.P. Single-nucleotide polymorphism identification assays using a thermostable DNA polymerase and delayed extraction MALDI-TOF mass spectrometry. Genome Res. 7 (1997) 378-388
10. Ikryannikova L.N., Afanas'ev M.V., Akopian T.A., Il'ina E.N., Kuz'min A.V., Larionova E.E., Smirnova T.G., Chernousova L.N., and Govorun V.M. Mass-spectrometry based minisequencing method for the rapid detection of drug resistance in Mycobacterium tuberculosis. J. Microbiol. Methods 70 (2007) 395-405
11. Ilina E.N., Malakhova M.V., Generozov E.V., Nikolaev E.N., and Govorun V.M. Matrix-assisted laser desorption ionization-time of flight (mass spectrometry) for hepatitis C virus genotyping. J. Clin. Microbiol. 43 (2005) 2810-2815
12. Jacoby G.A., and Sutton L. Properties of plasmids responsible for production of extended-spectrum beta-lactamases. Antimicrob.Agents Chemother. 35 (1991) 164-169
13. Leflon-Guibout V., Heym B., and Nicolas-Chanoine M. Updated sequence information and proposed nomenclature for bla(TEM) genes and their promoters. Antimicrob. Agents Chemother. 44 (2000) 3232-3234
14. Mabilat C., and Goussard S. PCR detection and identification of genes for extended-spectrum β-lactamases. In: Persing D., and Smith T. (Eds). Diagnostic Molecular Microbiology. Principles and Applications (1993), ASM, Washington 553-559
15. Nikulin A., Alexeev Y., and Edelstein M. A single-tube real-time PCR and melting-curve analysis for detection and characterisation of TEM-type extended-spectrum β-lactamases. Int. J. Antimicrob. Agents 29 (2007) S222
16. Paterson D.L. Resistance in Gram-negative bacteria: Enterobacteriaceae. Am. J. Med. 119 (2006) S20-S28
17. Vereshchagin V.A., Ilina E.N., Zubkov M.M., Priputnevich T.V., Kubanova A.A., and Govorun V.M. Detection of fluoroquinolone resistance single-nucleotide polymorphisms in Neisseria gonorrhoeae gyrA and parC using MALDI-TOF mass spectrometry. Mol. Biol. (Mosk.) 39 (2005) 806-814