Improving clone production for increased protein yield from mammalian cell lines

Innovations in Pharmaceutical Technology

Volumn , Issue 26, 2008, Pages 52-56

  Williams, Steve ^a   Cranenburgh, Rocky ^a  

^a Cobra Biomanufacturing Plc ^*

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ARTIFICIAL CHROMOSOME; BIOTECHNOLOGICAL PRODUCTION; CELL CULTURE; CELL LINE; CHROMATIN STRUCTURE; DNA INTEGRATION; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; GENE TARGETING; GENETIC SELECTION; GENETIC TRANSFECTION; HIGH THROUGHPUT SCREENING; INTERMETHOD COMPARISON; MAMMAL CELL; MOLECULAR CLONING; NONHUMAN; PROCESS DEVELOPMENT; PRODUCTIVITY; TRANSGENE;

EID: 55049121474     PISSN: 14717204     EISSN: None     Source Type: Trade Journal    
DOI: None     Document Type: Article

References (5)

1. Barnes LM and Dickson AJ, Mammalian cell factories for efficient and stable protein expression, Current Opinion in Biotechnology 17, pp381-386, 2006
2. Lindenbaum M, Perkins E, Csonka E, et al, A mammalian artificial chromosome engineering system (ACE System) applicable to biopharmaceutical protein production, transgenesis and gene-based cell therapy, Nucleic Acids Research 32, p21, 2004
3. Fisch I, The Role of Matrix-Attachment Regions in Increasing Recombinant Protein Expression, Bioprocess International, February 2007
4. Antoniou M, Harland L, Mustoe T, et al, Transgenes encompassing dual-promoter CpG islands from the human TBP and HNRPA2B1 loci are resistant to heterochromatin-mediated silencing, Genomics 82, pp269-279, 2003
5. Williams S, Mustoe T, Mulcahy T et al, CpG-island fragments from the HNRPA2B1/CBX3 genomic locus reduce silencing and enhance transgene expression from the hCMV promoter/enhancer in mammalian cells, BMC Biotechnology, 5, 17, 2005