A single Sec61-complex functions as a protein-conducting channel

Biochimica et Biophysica Acta - Molecular Cell Research

Volumn 1783, Issue 12, 2008, Pages 2375-2383

  Kalies, Kai Uwe ^a   Stokes, Vivica ^a   Hartmann, Enno ^a  

^a UNIVERSITY OF LÜBECK   (Germany)

Author keywords

Cotranslational protein translocation; ER membrane; Protein conducting channel; Ribosome binding; Sec61

Indexed keywords

CHYMOTRYPSIN; MEMBRANE PROTEIN; MESSENGER RNA; POLYPEPTIDE; POTASSIUM CHLORIDE; PROTEINASE; PUROMYCIN; SEC61 PROTEIN; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; COMPLEX FORMATION; CRYSTAL STRUCTURE; ENDOPLASMIC RETICULUM; ENZYME ASSAY; MEMBRANE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; RIBOSOME; RNA TRANSLATION;

ANIMALS; DOGS; ENDOPLASMIC RETICULUM, ROUGH; GENE TARGETING; ION CHANNELS; MEMBRANE PROTEINS; PANCREAS; PEPTIDE CHAIN ELONGATION, TRANSLATIONAL; PROTEIN BIOSYNTHESIS; PROTEIN TRANSPORT; RIBOSOMES; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 55049120190     PISSN: 01674889     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamcr.2008.08.005     Document Type: Article

References (41)

1. Johnson A.E., and van Waes M.A. The translocon: a dynamic gateway at the ER membrane. Annu. Rev. Cell Dev. Biol. 15 (1999) 799-842
2. Osborne A.R., Rapoport T.A., and van den Berg B. Protein translocation by the Sec61/SecY channel. Annu. Rev. Cell Dev. Biol. 21 (2005) 529-550
3. Keenan R.J., Freymann D.M., Stroud R.M., and Walter P. The signal recognition particle. Annu. Rev. Biochem. 70 (2001) 755-775
4. Wild K., Halic M., Sinning I., and Beckmann R. SRP meets the ribosome. Nat. Struct. Mol. Biol. 11 (2004) 1049-1053
5. Shan S.O., and Walter P. Co-translational protein targeting by the signal recognition particle. FEBS Lett. 579 (2005) 921-926
6. Kalies K.U., Görlich D., and Rapoport T.A. Binding of ribosomes to the rough endoplasmic reticulum mediated by the Sec61p-complex. J. Cell Biol. 126 (1994) 925-934
7. Jungnickel B., and Rapoport T.A. A posttargeting signal sequence recognition event in the endoplasmic reticulum membrane. Cell 82 (1995) 261-270
8. Matlack K.E., Mothes W., and Rapoport T.A. Protein translocation: tunnel vision. Cell 92 (1998) 381-390
9. Görlich D., and Rapoport T.A. Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane. Cell 75 (1993) 615-630
10. Mothes W., Prehn S., and Rapoport T.A. Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane. EMBO J. 13 (1994) 3973-3982
11. Prinz A., Behrens C., Rapoport T.A., Hartmann E., and Kalies K.U. Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA. EMBO J. 19 (2000) 1900-1906
12. Raden D., Song W., and Gilmore R. Role of the cytoplasmic segments of Sec61alpha in the ribosome-binding and translocation-promoting activities of the Sec61 complex. J. Cell Biol. 150 (2000) 53-64
13. Pohlschröder M., Prinz W.A., Hartmann E., and Beckwith J. Protein translocation in the three domains of life: variations on a theme. Cell 91 (1997) 563-566
14. Oliver D.B., and Beckwith J. Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli. J. Bacteriol. 150 (1982) 686-691
15. Müller M., Koch H.G., Beck K., and Schäfer U. Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane. Prog. Nucleic Acid Res. Mol. Biol. 66 (2001) 107-157
16. Pohlschröder M., Gimenez M.I., and Jarrell K.F. Protein transport in Archaea: Sec and twin arginine translocation pathways. Curr. Opin. Microbiol. 8 (2005) 713-719
17. Van den Berg B., Clemons Jr. W.M., Collinson I., Modis Y., Hartmann E., Harrison S.C., and Rapoport T.A. X-ray structure of a protein-conducting channel. Nature 427 (2004) 36-44
18. Hanein D., Matlack K.E., Jungnickel B., Plath K., Kalies K.U., Miller K.R., Rapoport T.A., and Akey C.W. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation. Cell 87 (1996) 721-732
19. Menetret J.F., Hegde R.S., Heinrich S.U., Chandramouli P., Ludtke S.J., Rapoport T.A., and Akey C.W. Architecture of the ribosome-channel complex derived from native membranes. J. Mol. Biol. 348 (2005) 445-457
20. Menetret J.F., Neuhof A., Morgan D.G., Plath K., Radermacher M., Rapoport T.A., and Akey C.W. The structure of ribosome-channel complexes engaged in protein translocation. Mol. Cell 6 (2000) 1219-1232
21. Beckmann R., Spahn C.M., Eswar N., Helmers J., Penczek P.A., Sali A., Frank J., and Blobel G. Architecture of the protein-conducting channel associated with the translating 80S ribosome. Cell 107 (2001) 361-372
22. Morgan D.G., Menetret J.F., Neuhof A., Rapoport T.A., and Akey C.W. Structure of the mammalian ribosome-channel complex at 17A resolution. J. Mol. Biol. 324 (2002) 871-886
23. Meyer T.H., Menetret J.F., Breitling R., Miller K.R., Akey C.W., and Rapoport T.A. The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex. J. Mol. Biol. 285 (1999) 1789-1800
24. Manting E.H., van Der Does C., Remigy H., Engel A., and Driessen A.J. SecYEG assembles into a tetramer to form the active protein translocation channel. EMBO J. 19 (2000) 852-861
25. Yahr T.L., and Wickner W.T. Evaluating the oligomeric state of SecYEG in preprotein translocase. EMBO J. 19 (2000) 4393-4401
26. Cannon K.S., Or E., Clemons Jr. W.M., Shibata Y., and Rapoport T.A. Disulfide bridge formation between SecY and a translocating polypeptide localizes the translocation pore to the center of SecY. J. Cell Biol. 169 (2005) 219-225
27. Menetret J.F., Schaletzky J., Clemons Jr. W.M., Osborne A.R., Skanland S.S., Denison C., Gygi S.P., Kirkpatrick D.S., Park E., Ludtke S.J., Rapoport T.A., and Akey C.W. Ribosome binding of a single copy of the SecY complex: implications for protein translocation. Mol. Cell 28 (2007) 1083-1092
28. Bessonneau P., Besson V., Collinson I., and Duong F. The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure. EMBO J. 21 (2002) 995-1003
29. Lipp J., and Dobberstein B. Signal and membrane anchor functions overlap in the type II membrane protein I gamma CAT. J. Cell Biol. 106 (1988) 1813-1820
30. Mitra K., Schaffitzel C., Shaikh T., Tama F., Jenni S., Brooks III C.L., Ban N., and Frank J. Structure of the E. coli protein-conducting channel bound to a translating ribosome. Nature 438 (2005) 318-324
31. Walter P., and Blobel G. Preparation of microsomal membranes for cotranslational protein translocation. Methods Enzymol. 96 (1983) 84-93
32. Collins P.G., and Gilmore R. Ribosome binding to the endoplasmic reticulum: a 180-kD protein identified by crosslinking to membrane-bound ribosomes is not required for ribosome binding activity. J. Cell Biol. 114 (1991) 639-649
33. Görlich D., Prehn S., Hartmann E., Kalies K.U., and Rapoport T.A. A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation. Cell 71 (1992) 489-503
34. Neuhof A., Rolls M.M., Jungnickel B., Kalies K.U., and Rapoport T.A. Binding of signal recognition particle gives ribosome/nascent chain complexes a competitive advantage in endoplasmic reticulum membrane interaction. Mol. Biol. Cell 9 (1998) 103-115
35. Adelman M.R., Sabatini D.D., and Blobel G. Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components. J. Cell Biol. 56 (1973) 206-229
36. Kalies K.U., Rapoport T.A., and Hartmann E. The beta subunit of the Sec61 complex facilitates cotranslational protein transport and interacts with the signal peptidase during translocation. J. Cell Biol. 141 (1998) 887-894
37. Borgese N., Mok W., Kreibich G., and Sabatini D.D. Ribosomal-membrane interaction: in vitro binding of ribosomes to microsomal membranes. J. Mol. Biol. 88 (1974) 559-580
38. Connolly T., Collins P., and Gilmore R. Access of proteinase K to partially translocated nascent polypeptides in intact and detergent-solubilized membranes. J. Cell Biol. 108 (1989) 299-307
39. Matlack K.E., and Walter P. The 70 carboxyl-terminal amino acids of nascent secretory proteins are protected from proteolysis by the ribosome and the protein translocation apparatus of the endoplasmic reticulum membrane. J. Biol. Chem. 270 (1995) 6170-6180
40. Schaletzky J., and Rapoport T.A. Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane. Mol. Biol. Cell 17 (2006) 3860-3869
41. Menetret J.F., Hegde R.S., Aguiar M., Gygi S.P., Park E., Rapoport T.A., and Akey C.W. Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome. Structure 16 (2008) 1126-1137