Rapid affinity-based fingerprinting of 14-3-3 isoforms using a combinatorial peptide microarray

Angewandte Chemie - International Edition

Volumn 47, Issue 39, 2008, Pages 7438-7441

  Lu, Candy H S ^a   Sun, Hongyan ^a   Abu Bakar, Farhana B ^a   Uttamchandani, Mahesh ^a   Zhou, Wei ^a   Liou, Yih Cherng ^a   Yao, Shao Q ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

Combinatorial chemistry; Isoforms; Microarrays; Peptides; Proteins

Indexed keywords

MAMMALS;

COMBINATORIAL CHEMISTRY; ISOFORMS; MICROARRAYS; PEPTIDES; PROTEINS;

AMINES;

ISOPROTEIN; PEPTIDE; PROTEIN 14 3 3;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; METHODOLOGY; PROTEIN MICROARRAY; TIME;

14-3-3 PROTEINS; MOLECULAR STRUCTURE; PEPTIDES; PROTEIN ARRAY ANALYSIS; PROTEIN ISOFORMS; TIME FACTORS;

EID: 54749085344     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200801395     Document Type: Article

References (26)

1. a) H. Hermeking, Nat. Rev. Cancer 2003, 3, 931-943;
2. b) P. Mhawech, Cell Res. 2005, 15, 228-236.
3. a) M. B. Yaffe, K. Rittinger, S. Volinia, P. R. Carson, A. Aitken, H. Leffers, S. J. Gamblin, S. J. Smerdon, L. C. Cantley, Cell 1997, 91, 961-971;
4. b) X. Yang, W. H. Lee, F. Sobott, E. Papagrigoriou, C. V. Robinson, J. G. Grossmann, M. Sundstrom, D. A. Doyle, J. M. Elkins, Proc. Natl. Acad. Sci. USA 2006, 103, 17237-17242;
5. c) E. W. Wilker, R. A. Grant, S. C. Artim, M. B. Yaffe, J. Biol. Chem. 2005, 280, 18891-18898.
6. a) G. Henderson, M. Bradley, Curr. Opin. Biotechnol. 2007, 18, 326-330;
7. b) K. Y. Tomizaki, K. Usui, H. Mihara, ChemBioChem 2005, 6, 783-799;
8. c) R. C. Panicker, X. Huang, S. Q. Yao, Comb. Chem. High Throughput Screening 2004, 7, 547-556;
9. d) K. S. Lam, M. Renil, Curr. Opin. Chem. Biol. 2002, 6, 353-358;
10. e) M. Köhn, M. Gutierrez-Rodriguez, P. Jonkheijm, S. Wetzel, R. Wacker, H. Schroeder, H. Prinz, C. M. Niemeyer, R. Breinbauer, S. E. Szedlacsek, H. Waldmann, Angew. Chem. 2007, 119, 7844-7847;
11. Angew. Chem. Int. Ed. 2007, 46, 7700-7703;
12. f) M. Schutkowski, U. Reineke, U. Reimer, ChemBioChem 2005, 6, 513-521.
13. a) J. Pelletier, S. Sidhu, Curr. Opin. Biotechnol. 2001, 12, 340-347;
14. b) R. Frank, Tetrahedron 1992, 48, 9217-9232;
15. c) R. A. Houghten, D. B. Wilson, C. Pinilla, Drug Discovery Today 2000, 5, 276-285;
16. d) K. S. Lam, M. Lebl, V. Krchnak, Chem. Rev. 1997, 97, 411-448.
17. 3 compounds with our fragment-based approach if the same number of AAs is used.
18. The previously reported combinatorial peptide microarray (M. Uttamchandani, E. W. S. Chan, G. Y. J. Chen, S. Q. Yao, Bioorg. Med. Chem. Lett. 2003, 13, 2997-3000), which introduced PS libraries on a glass slide, failed because of the extremely low concentrations of individual peptide sequences present in each spot.
19. a) M. Uttamchandani, W. L. Lee, J. Wang, S. Q. Yao, J. Am. Chem. Soc. 2007, 129, 13110-13117;
20. b) H. Sun, C. H. S. Lu, M. Uttamchandani, Y. Xia, Y.-C. Liou, S. Q. Yao, Angew. Chem. 2008, 120, 1722-1726;
21. Angew. Chem. Int. Ed. 2008, 47, 1698-1702.
22. K. P. Lu, Y.-C. Liou, X. Z. Zhou, Trends Cell Biol. 2002, 12, 164-172.
23. These two motifs are similar to the known 14-3-3 binding motif, RPVSSAApSVY, previously reported (N. Ku, J. Liao, M. B. Omary, EMBO J. 1998, 17, 1892-1906).
24. K. T. Pilobello, D. E. Slawek, L. K. Mahal, Proc. Natl. Acad. Sci. USA 2007, 104, 11534-11539.
25. Our dual-color ratiometric approach was possible because 14-3-3σ, unlike the other six 14-3-3 isoforms, is known to only form a homodimer. Therefore an equal mixture of σ/isoform should only give rise to the expected σ/σ and isoform/isoform dimers upon phosphopeptide binding. See reference [2c] for details.
26. H. Sun, S. Chattopadhaya, J. Wang, S. Q. Yao, Anal. Bioanal. Chem. 2006, 386, 416-426.