The crystal structure of MexR from Pseudomonas aeruginosa in complex with its antirepressor ArmR

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 39, 2008, Pages 14832-14837

  Wilke, Mark S ^a   Heller, Markus ^a   Creagh, A Louise ^a,b   Haynes, Charles A ^a,b   McIntosh, Lawrence P ^a,b,c   Poole, Keith ^c   Strynadka, Natalie C J ^a,b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c QUEEN S UNIVERSITY   (Canada)

Author keywords

Gene regulation; MarR; MexAB oprM; PA3719; Protein peptide

Indexed keywords

ARMR PROTEIN; MEXR PROTEIN; PROTEIN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; MEXR PROTEIN, PSEUDOMONAS AERUGINOSA; REPRESSOR PROTEIN;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; COMPARATIVE STUDY; CONTROLLED STUDY; CRYSTAL STRUCTURE; DNA BINDING; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; PSEUDOMONAS AERUGINOSA; CHEMISTRY; CRYSTALLIZATION; DIMERIZATION; DRUG ANTAGONISM; METABOLISM; PROTEIN SECONDARY STRUCTURE;

PSEUDOMONAS AERUGINOSA;

BACTERIAL PROTEINS; CRYSTALLIZATION; DIMERIZATION; PROTEIN STRUCTURE, SECONDARY; PSEUDOMONAS AERUGINOSA; REPRESSOR PROTEINS;

EID: 54449100560     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0805489105     Document Type: Article

References (41)

1. Mima T, Sekiya H, Mizushima T, Kuroda T, Tsuchiya T (2005) Gene cloning and properties of the RND-type multidrug efflux pumps MexPQ-OpmE and MexMN-OprM from Pseudomonas aeruginosa. Microbiol Immunol 49:999-1002.
2. Mima T, Joshi S, Gomez-Escalada M, Schweizer HP (2007) Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins. J Bacteriol 189:7600-7609.
3. Poole K, Srikumar R (2001) Multidrug efflux in. Pseudomonas aeruginosa: Components, mechanisms, and clinical significance. Curr Top Med Chem 1:59-71.
4. Seeger MA, et al. (2006) Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism. Science 313:1295-1298.
5. Murakami S, Nakashima R, Yamashita E, Matsumoto T, Yamaguchi A (2006) Crystal structures of amultidrug transporter reveal a functionally rotating mechanism. Nature 443:173-179.
6. Muller JF, Stevens AM, Craig J, Love NG (2007) Transcriptome analysis reveals that multidrug efflux genes are up-regulated to protect Pseudomonas aeruginosa from pentachlorophenol stress. Appl Environ Microbiol 73:4550-4558.
7. Saito K, Yoneyama H, Nakae T (1999) nalB-type mutations causing the overexpression of the MexAB-OprM efflux pump are located in the mexR gene of the Pseudomonas aeruginosa chromosome. FEMS Microbiol Lett 179:67-72.
8. Srikumar R, Paul CJ, Poole K (2000) Influence of mutations in the mexR repressor gene on expression of the MexA-MexB-oprM multidrug efflux system of Pseudomonas aeruginosa. J Bacteriol 182:1410-1414.
9. Cao L, Srikumar R, Poole K (2004) MexAB-OprM hyperexpression in NalC-type multidrug-resistant Pseudomonas aeruginosa: Identification and characterization of the nalC gene encoding a repressor of PA3720-PA3719. Mol Microbiol 53:1423-1436.
10. Llanes C, et al. (2004) Clinical strains of Pseudomonas aeruginosa overproducing MexAB-OprM and MexXY efflux pumps simultaneously. Antimicrob Agents Chemother 48:1797-1802.
11. Sobel ML, Hocquet D, Cao L, Plesiat P, Poole K (2005) Mutations in. PA3574 (nalD) lead to increased MexAB-OprM expression and multidrug resistance in laboratory and clinical isolates of Pseudomonas aeruginosa. Antimicrob Agents Chemother 49:1782-1786.
12. Boutoille D, et al. (2004) Detection of an IS21 insertion sequence in the mexR gene of Pseudomonas aeruginosa increasing β-lactam resistance. FEMS Microbiol Lett 230:143-146.
13. Morita Y, Cao L, Gould VC, Avison MB, Poole K (2006) nalD encodes a second repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa. J Bacteriol 188:8649-8654.
14. Evans K, Adewoye L, Poole K (2001) MexR repressor of the. mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa: Identification of MexR binding sites in the mexA-mexR intergenic region. J Bacteriol 183:807-812.
15. Saito K, Eda S, Maseda H, Nakae T (2001) Molecular mechanism of MexR-mediated regulation of MexAB-OprM efflux pump expression in Pseudomonas aeruginosa. FEMS Microbiol Lett 195:23-28.
16. Daigle DM, et al. (2007) A protein modulator of multidrug efflux gene expression in Pseudomonas aeruginosa. J Bacteriol 189:5441-5451.
17. Wilkinson SP, GroveA(2006) Ligand-responsive transcriptional regulation by members of the MarR family of winged helix proteins. Curr Issues Mol Biol 8:51-62.
18. Ellison DW, Miller VL (2006) Regulation of virulence by members of the MarR/SlyA family. Curr Opin Microbiol 9:153-159.
19. Alekshun MN, Levy SB, Mealy TR, Seaton BA, Head JF (2001) The crystal structure of MarR, a regulator of multiple antibiotic resistance, at 2.3-A resolution. Nat Struct Biol 8:710-714.
20. Newberry KJ, Fuangthong M, Panmanee W, Mongkolsuk S, Brennan RG (2007) Structural mechanism of organic hydroperoxide induction of the transcription regulator OhrR. Mol Cell 28:652-664.
21. Bordelon T, Wilkinson SP, Grove A, Newcomer ME (2006) The crystal structure of the transcriptional regulator HucR from Deinococcus radiodurans reveals a repressor preconfigured for DNA binding. J Mol Biol 360:168-177.
22. Kumarevel T, et al. (2008) Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7. J Struct Biol 161:9-17.
23. Hong M, Fuangthong M, Helmann JD, Brennan RG (2005) Structure of an OhrR-ohrA operator complex reveals the DNA binding mechanism of the MarR family. Mol Cell 20:131-141.
24. Saridakis V, Shahinas D, Xu X, Christendat D (2008) Structural insight on the mechanism of regulation of the MarR family of proteins: High-resolution crystal structure of a transcriptional repressor from Methanobacterium thermoautotrophicum. J Mol Biol 377:655-667.
25. Lim D, Poole K, Strynadka NC (2002) Crystal structure of the MexR repressor of the. mexRAB-oprM multidrug efflux operon of Pseudomonas aeruginosa. J Biol Chem 277:29253-29259.
26. Yamada H, et al. (2007) "Crystal lattice engineering," an approach to engineer protein crystal contacts by creating intermolecular symmetry: Crystallization and structure determination of a mutant human RNase 1 with a hydrophobic interface of leucines. Protein Sci 16:1389-1397.
27. Baumgarth B, Bartels FW, Anselmetti D, Becker A, Ros R (2005) Detailed studies of the binding mechanism of the Sinorhizobium meliloti transcriptional activator ExpG to DNA. Microbiology 151:259-268.
28. Wilkinson SP, Grove A (2005) Negative cooperativity of uric acid binding to the transcriptional regulator HucR from Deinococcus radiodurans. J Mol Biol 350:617-630.
29. Saito K, Akama H, Yoshihara E, Nakae T (2003) Mutations affecting DNA-binding activity of the MexR repressor of mexR-mexA-mexB-oprM operon expression. J Bacteriol 185:6195-6198.
30. Chen PR, et al. (2006) An oxidation-sensing mechanism is used by the global regulator MgrA in Staphylococcus aureus. Nat Chem Biol 2:591-595.
31. Lee JW, Soonsanga S, Helmann JD (2007) A complex thiolate switch regulates the Bacillus subtilis organic peroxide sensor OhrR. Proc Natl Acad Sci USA 104:8743-8748.
32. Soonsanga S, Lee JW, Helmann JD (2008) Oxidant-dependent switching between reversible and sacrificial oxidation pathways for Bacillus subtilis OhrR. Mol Microbiol 20:131-141.
33. Pace CN, Vajdos F, Fee L, Grimsley G, Gray T (1995) How to measure and predict the molar absorption coefficient of a protein. Protein Sci 4:2411-2423.
34. Otwinowski Z, Minor W (1997) Processing of x-ray diffraction data collected in oscillation mode. Methods Enzymol 276:307-326.
35. McCoy AJ, et al. (2007) Phaser crystallographic software. J App Crystallgr 40:658-674.
36. Vagin AA, Teplyakov A (1997) MOLREP: An automated program for molecular replacement. J Appl Crystallgr 30:1022-1025.
37. Emsley P, Cowtan K (2004) Coot: Model-building tools for molecular graphics. Acta Crystallogr D 60:2126-2132.
38. Adams PD, et al. (2002) PHENIX: Building new software for automated crystallographic structure determination. Acta Crystallogr D 58:1948-1954.
39. Collaborative Computational Project No. 4 (1994) The CCP4 suite programs for protein crystallography. Acta Crystallogr D 50:760-763.
40. DeLano WL (2002) The PyMOL Molecular Graphics System (DeLano Scientific, San Carlos, CA).
41. Pettersen EF, et al. (2004) UCSF Chimera: A visualization system for exploratory research and analysis. J Comput Chem 25:1605-1612.