Production of a secreted glycoprotein from an inducible promoter system in a perfusion bioreactor

Biotechnology Progress

Volumn 20, Issue 5, 2004, Pages 1402-1407

  Lipscomb, Matthew L ^a   Mowry, Mark C ^a   Kompala, Dhinakar S ^a  

^a UCB 450   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; BIOREACTORS; CELL CULTURE; METABOLISM;

CHINESE HAMSTER OVARY (CHO) CELLS; GLYCOPROTEINS; PROMOTER EXPRESSION SYSTEM;

PROTEINS;

CRICETINAE; CRICETULUS GRISEUS; MOUSE MAMMARY TUMOR VIRUS;

EID: 5444273568     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp049973j     Document Type: Article

References (21)

1. Gossen, M.; Bujard, H. Tight control of gene expression in mammalian cells by tetracycline- responsive promoters. Proc. Natl. Acad. Sci. 1992, 89, 5547-5551.
2. Gossen, M.; Bonin, A. L.; Freundlieb, S.; Bujard, H. Inducible gene expression systems for higher eukaryotic cells. Curr. Opin. Biotechnol. 1994, 5, 516-520.
3. No, D.; Yao, T. P.; Evans, R. M. Ecdysone-inducible gene expression in mammalian cells and transgenic mice. Proc. Natl. Acad. Sci. 1996, 93, 3346-3351.
4. Wyborski, D. L.; Short, J. M. Analysis of inducers of the E. coli lac repressor system in mammalian cells and whole animals. Nucleic Acids Res. 1991, 19, 4647-4653.
5. Ko, M. S. H.; Takahashi, N.; Sugiyama, N.; Takano, T. An auto-inducible vector conferring high glucocorticoid inducibility upon stable transformant cells. Gene 1989, 84, 383-389.
6. Yamamoto, K. R. Steroid receptor regulated transcription of specific genes and gene networks. Annu. Rev. Genet. 1985, 19, 209-252.
7. Vanderbilt, J. N.; Miesfeld, R.; Maler, B. A.; Yamamoto, K. R. Intracellular receptor concentration limits glucocorticoid-dependent enhancer activity. Mol. Endocrinol. 1987, 1, 68-74.
8. Gu, M. B.; Todd, P.; Kompala, D. S. Growth and induction kinetics of inducible and autoinducible expression of heterologous protein in suspension cultures of recombinant mouse L cell lines. Biotechnol. Prog. 1996, 12, 226-233.
9. Woodside S. M.; Bowen, B. D.; Piret, J. M. Mammalian cell retention devices for stirred perfusion bioreactors. Cytotechnology 1998, 28, 163-175.
10. Himmelfarb, P.; Thayer, P. S.; Martin, H. E. Spin filter culture: the propagation of mammalian cells in suspension. Science 1969, 164, 555-557.
11. Batt, B. C.; Davis, R. H.; Kompala, D. S. Inclined sedimentation for selective retention of viable hybridomas in a continuous suspension bioreactor. Biotechnol. Prog. 1990, 6, 458-464.
12. James, R. I.; Elton, J. P.; Todd, P.; Kompala, D. S. Engineering CHO cells to overexpress a secreted reporter protein upon induction from mouse mammary tumor virus promoter. Biotechnol. Bioeng. 2000, 67, 134-40.
13. Searles, J. A.; Todd, P.; Kompala, D. S. Viable cell recycle with an inclined settler in the perfusion culture of suspended recombinant Chinese hamster ovary cells. Biotechnol. Prog. 1994, 10, 198-206.
14. Berger, J.; Hauber, J.; Hauber, R.; Geiger, R.; Cullen, B. R. Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 1988, 66, 1-10.
15. Glacken, M.; Flesichaker, R.; Sinskey, A. Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalia cells. Biotechnol. Bioeng. 1986, 28, 1376-1389.
16. Howe, J. R.; Skryabin, B. V.; Belcher, S. M.; Zerillo, C. A.; Schmauss, C. The responsiveness of a tetracycline-sensitive expression system differs in different cell lines. J. Biol. Chem. 1995, 270, 23, 14168-14174.
17. Mangalampalli, V.; Mowry, M.; Lipscomb, M.; James, R.; Johnson, A.; Kompala, D. Increased production of a secreted glycoprotein in engineered CHO cells through amplification of a transcription factor. Cytotechnology 2002, 38, 23-35.
18. Banik, G. G.; Todd, P. W.; Kompala, D. S. Foreign protein expression from S phase specific promoters in continuous cultures of recombinant CHO cells. Cytotechnology 1996, 22, 179-184.
19. Lee, F. W. F.; Elias, C. B.; Todd, P. W.; Kompala, D. S. Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth-associated production of a foreign protein, β-galactosidase. Cytotechnology 1998, 28, 73-80.
20. Kompala, D. S. Cell Growth and Protein Expression Kinetics. In Encyclopedia of Cell Technology; Spier, R., Ed.; John Wiley, 2000; pp 383-392.
21. Barnes, L. M.; Bentley, C. M.; Dickson, A. J. Stability of protein production from recombinant mammalian cells. Biotechnol. Bioeng. 2002, 81, 6, 631-639.