Evaluation of a real-time fluorescent PCR assay for rapid detection of Group B Streptococci in neonatal blood

Diagnostic Microbiology and Infectious Disease

Volumn 50, Issue 1, 2004, Pages 7-13

  Golden, Stephen M ^a   Stamilio, David M ^b   Faux, Brian M ^a   Dela Cruz, Wilfred P ^a   Shoemaker, Craig T ^c   Blackmon, Camille L ^a   Stassen, Sarah D ^a   Clark, Velvet M ^a   Smith, James W ^a   Johnson, Oswald L ^a  

^a DAVID GRANT MEDICAL CENTER   (United States)

^b HOSPITAL OF THE UNIVERSITY OF PENNSYLVANIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA;

ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BLOOD SAMPLING; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA SEQUENCE; FLUORESCENCE; GENE TARGETING; HUMAN; HYBRIDIZATION; LABORATORY DIAGNOSIS; NEWBORN; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; STREPTOCOCCUS AGALACTIAE;

BASE SEQUENCE; BLOTTING, SOUTHERN; DNA, BACTERIAL; ELECTROPHORESIS, AGAR GEL; FEMALE; HUMANS; INFANT, NEWBORN; MALE; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS AGALACTIAE; TIME FACTORS;

EID: 5444234119     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2004.04.021     Document Type: Article

References (10)

1. Bergeron M.G., Ke D., et al. Rapid detection of group B streptococci in pregnant women at delivery. N Engl J Med. 343:2000;175-179
2. Craft D. Emergent technologies: Direct microbial antigen detection. Mahon C.R., Manuselis G. Text Book of Diagnostic Microbiology. 2000;141-151 W.B. Saunders Co., Philadelphia, PA
3. Gerdes J.S. Clinicopathologic approach to the diagnosis of sepsis. Clin Perinatol. 18:1999;361-381
4. Ke D., Bergeron M.G. Molecular methods for rapid detection of group B streptococci. Expert Rev Mol Diagn. 1:2001;175-181
5. Ke D., Menard C., et al. Development of conventional and real-time PCR assays for the rapid detection of group B streptococci. Clin Chem. 46:2000;331-342
6. Larsen H.S. Streptococcaceae. Mahon C.R., Manuselis G. Textbook of Diagnostic Microbiology. 2000;361-361 W.B. Saunders Co., Philadelphia, PA
7. Riest G., Linde H.J., et al. Comparison of BacT/Alert and BACTEC NR 860 blood culture systems in a laboratory not continuously staffed. Clin Microbiol Infect. 3:1997;345-351
8. Schuchat A. Neonatal group B streptococcal disease - screening and prevention. N Engl J Med. 343:2000;209-210
9. Washington J.A., Ilstrup D.M. Blood cultures Issues and controversies. Rev Infect Dis. 8:1986;792-802
10. Wegman M. Annual summary of vital statistics. Pediatrics. 92:1993;743-754