Dynamic imaging of calcium and STIM1 in the same cell using wide-field and TIRF microscopy

BioTechniques

Volumn 45, Issue 3, 2008, Pages 347-348

  Walker, Simon ^a   Cunniffe, Nicholas ^a   Bootman, Martin ^a   Roderick, H Llewelyn ^a,b  

^a BABRAHAM INSTITUTE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CELL SURFACE PROTEIN; STROMAL INTERACTION MOLECULE 1; UNCLASSIFIED DRUG;

ARTICLE; CALCIUM SIGNALING; CALCIUM TRANSPORT; CELL LABELING; CELL MEMBRANE; CELL TRANSPORT; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; KIDNEY CELL; MOLECULAR IMAGING; PROTEIN TRANSPORT;

CALCIUM; CALCIUM SIGNALING; CELL LINE; CELL MEMBRANE; FLUORESCENT DYES; FURA-2; HUMANS; KIDNEY; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; NEOPLASM PROTEINS; THAPSIGARGIN; TRANSFECTION;

EID: 54349106363     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112957     Document Type: Article

References (5)

1. Clapham D.E. Calcium Signaling. Cell 2007; 131: 1047-1058.
2. 2+ Channel Function. The Journal of Cell Biology 2005; 169(3): 435-445.
3. Luik R.M., Wang B., Prakriya M., Wu M.M., and Lewis R.S. Oligomerization of STIM1 Couples ER Calcium Depletion to CRAC Channel Activation. Nature Letters 2008: 1-7.
4. Liou J., Kim M.L., Heo W.D., Jones J.T., Myers J.W., Ferrell J.E. and Meyer T. STIM1 is a Ca2+ Sensor Essential for Ca2+-Store-Depletion-Triggered Ca2+ Influx. Current Biology 2005; 15: 1235-1241.
5. Brandman O., Liou J., Park W.S., Meyer T. STIM2 Is a Feedback Regulator that Stabilizes Basal Cytosolic and Endoplasmic Reticulum Ca(2+) Levels. Cell 2007; 131(7): 1327-29.