Batch and size-exclusion chromatographic characterization of ultra-high molar mass sodium hyaluronate containing low amounts of strongly scattering impurities by dual low angle light scattering/refractometric detection

Journal of Liquid Chromatography and Related Technologies

Volumn 31, Issue 20, 2008, Pages 3077-3093

  Porsch, Bedřich ^a   Laga, Richard ^a   Koňák, Čestmír ^a  

^a INSTITUTE OF MACROMOLECULAR CHEMISTRY   (Czech Republic)

Author keywords

Dual low angle light scattering refractometric detection; Size exclusion chromatography; Sodium hyaluronate; Static light scattering; Strongly scattering impurities

Indexed keywords

ADSORPTION; BIOLOGICAL MATERIALS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; COLUMN CHROMATOGRAPHY; COLUMNS (STRUCTURAL); CONCENTRATION (PROCESS); ELECTROMAGNETIC WAVES; FILTERS (FOR FLUIDS); FLOW RATE; FLUID DYNAMICS; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBIC CHROMATOGRAPHY; HYDROPHOBICITY; HYDROXYLATION; IMAGE SEGMENTATION; INJECTION (OIL WELLS); LIGHT SCATTERING; MOLAR MASS; NANOSTRUCTURED MATERIALS; ORGANIC POLYMERS; POLYDISPERSITY; POLYVINYL ALCOHOLS; POROUS MATERIALS; REFRACTION; SCATTERING; SODIUM; SODIUM CHLORIDE; WAVE FILTERS;

BULK LIGHT SCATTERINGS; CHROMATOGRAPHIC CHARACTERIZATIONS; DUAL LOW-ANGLE LIGHT SCATTERING/REFRACTOMETRIC DETECTION; HYDRODYNAMIC FLOWS; HYDROPHOBIC ADSORPTIONS; HYDROPHOBIC FILTERS; LOW ANGLE LIGHT SCATTERINGS; MASS SCALES; MOLAR MASS DISTRIBUTIONS; POLYDISPERSITY INDICES; REFRACTOMETRIC DETECTIONS; SEC ANALYSIS; SIZE EXCLUSIONS; SLALOM CHROMATOGRAPHIES; SODIUM HYALURONATE; STATIC LIGHT SCATTERING; STRONGLY SCATTERING IMPURITIES;

SIZE EXCLUSION CHROMATOGRAPHY;

HYALURONIC ACID;

ADSORPTION; ARTICLE; DEGRADATION; FLOW RATE; GEL PERMEATION CHROMATOGRAPHY; HYDRODYNAMICS; HYDROPHOBICITY; LIGHT SCATTERING; POROSITY; REFRACTOMETRY;

EID: 54349096843     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826070802479984     Document Type: Article

References (27)

1. Lapčík, Jr., L.; Lapčík, L.; De Smedt, S.; Demeester, J.; Chabreček, P. Hyaluronan: Preparation, structure, properties, and applications. Chem. Rev. 1998, 98 (8), 2663-2684.
2. Morra, M. Engineering of biomaterials surfaces by hyaluronan. Biomacromolecules 2005, 6 (3), 1205-1223.
3. Caporossi, A.; Baiocchi, S.; Sforzi, C.; Frezzotti, R. Healon GV versus Healon in demanding cataract-surgery. J. Cataract Refract. Surg. 1995, 21 (6), 710-713.
4. Strobel, J. Comparison of space-maintaining capabilities of Healon and Healon GV during phacoemulsification. J. Cataract Refract. Surg. 1997, 23 (7), 1081-1084.
5. Giddings, J.C. Analysis of fundamental obstacles to the size exclusion chromatography of polymers of ultrahigh molecular weight. Adv. Chromatogr. 1982, 20, 217-258.
6. Takahashi, R.; Al-Assaf, S.; Williams, P.A.; Kubota, K.; Okamoto, A.; Nishinan, K. Asymmetrical-flow field-flow fractionation with on-line multiangle light scattering detection. 1. Application to wormlike chain analysis of weakly stiff polymer chains. Biomacromolecules 2003, 4 (2), 404-409.
7. Lee, H.; Cho, I-H.; Moon, M.H. Effect of dissolution temperature on the structures of sodium hyaluronate by flow field-flow fractionation/multiangle iangle light scattering. J. Chromatogr. A 2006, 1131 (1-2), 185-191.
8. Shin, D-Y.; Hwang, E.; Cho, I-H.; Moon, M.H. Molecular weight and structure characterization of sodium hyaluronate and its gamma radiation degradation products by flow field-flow fractionation and on-line multiangle light scattering. J. Cromatogr. A 2007, 1160 (1-2), 270-275.
9. Mes, E.P.C.; de Jonge, H.; Klein, T; Welz, R.R.; Gillespie, D.T. Characterization of high molecular weight polyethylenes using high temperature asymmetrical flow field-flow fractionation with on-line infrared, light scattering and viscometry detection. J. Chromatogr. A 2007, 1154 (1-2), 319-330.
10. Ghosh, S.; Li, X.; Reed, Ch.E.; Reed, W.F. Apparent persistence lengths and diffusion behavior of high molecular weight hyaluronate. Biopolymers 1990, 30 (11-12), 1101-1112.
11. Fouissac, E.; Milas, M.; Rinaudo, M.; Borsali, R. Influence of the ionic strength on the dimensions of sodium hyaluronate. Macromolecules 1992, 25 (21), 5613-5617.
12. Mendichi, R.; Schieroni, A.G. Fractionation and characterization of ultra-high molar mass hyaluronan: 2. On-line size exclusion chromatography methods. Polymer 2002, 43 (23), 6115-6121.
13. Shiedlin, A.; Bigelow, R.; Christopher, W.; Arbabi, S.; Yang, L.; Maier, R.V.; Wainwright, N.; Childs, A.; Miller, R.J. Evaluation of hyaluronan from different sources: Streptococcus zooepidermicus, rooster comb, bovine vitreous, and human umbilical cord. Biomacromolecules 2004, 5 (6), 2122-2127.
14. Buhler, E.; Boué, F. Chain persistence length and structure in hyaluronan solutions: Ionic strength dependence for a model semirigid polyelectrolyte. Macromolecules 2004, 37 (4), 1600-1610.
15. Porsch, B.; Welinder, A.; Körner, A.; Wittgren, B. Distribution analysis of ultra-high molecular mass poly(ethylene oxide) containing silica particles by size-exclusion chromatography with dual light-scattering and refractometric detection. J. Chromatogr. A 2005, 1068 (2), 249-260.
16. Mendichi, R.; Schieroni, A.G.; Grassi, C.; Re, A. Characterization of ultrahigh molar mass hyaluronan: 1. Off-line static methods. Polymer 1998, 39 (25), 6611-6620.
17. Nilsson, S.; Sundelöf, L.-O.; Porsch, B. On the characterization principles of some technically important water soluble non-ionic cellulose derivatives. Carbohydr. Polym. 1995, 28 (3), 265-275.
18. Porsch, B.; Nilsson, S.; Sundelöf, L.-O. Association of ethyl(hydroxyethyl)-cellulose solutions. Macromolecules 1997, 30 (16), 4626-4632.
19. Grznárová, G.; Polakovič, M.; Ačai, P.; Görner, T. Extra-column dispersion of macromolecular solutes in aqueous-phase size-exclusion chromatography. J. Chromatogr. A 2004, 1040 (1), 33-43.
20. Meehan, E. Flow injection analysis of polymeric excipients used in pharmaceutical formulations. Int. J. Polym. Anal. Charact. 2006, 11 (1) 35-45.
21. Bitter, T.; Muir, H.M. A modified uronic acid carbazole reaction. Anal. Biochem. 1962, 4 (4), 330-334.
22. Schimanowski, R.; Strelitzki, R.; Mullin, D.A.; Reed, W.F. Heterogeneous time dependent static light scattering. Macromolecules 1999, 32 (21), 7055-7063.
23. Strelitzki, R.; Reed, W.F. Automated batch characterization of polymer solutions by static light scattering and viscometry. J. Appl. Polym. Sci. 1999, 73 (12), 2359-2367.
24. Hirabayashi, J.; Kasai, K-I. Effect of DNA topology, temperature and solvent viscosity on DNA retardation in slalom chromatography. J. Chromatogr. A 2000, 893 (1), 115-122.
25. Liu, Y-G.; Radke, W.; Pasch, H. Coil-stretch transition of high molar mass polymers in packed-column hydrodynamic chromatography. Macromolecules 2005, 38 (17), 7476-7484.
26. Stegeman, G.; Kraak, J.C.; Poppe, H. Hydrodynamic and size-exclusion chromatography of polymers on porous particles. J. Chromatogr. A 1991, 550 (1-2), 721-739.
27. Mendichi, R.; Šoltés, L.; Schieroni, A.G. Evaluation of radius of gyration and intrinsic viscosity molar mass dependence and stiffness of hyaluronan. Biomacromolecules 2003, 4 (6), 1805-1810.