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Volumn 9, Issue 15, 2008, Pages 2452-2462

Resin-bound aminofluorescein for C-terminal labeling of peptides: High-affinity polarization probes binding to polyproline-specific GYF domains

Author keywords

Fluorescence polarization; GYF domains; Labeling; Proline rich sequences; Protein protein interactions

Indexed keywords

AMINOFLUORESCEIN; FLUORESCEIN; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; NITROGEN 15; PROLINE; RESIN; UNCLASSIFIED DRUG;

EID: 54349089639     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.200800329     Document Type: Article
Times cited : (11)

References (31)
  • 6
    • 76949127690 scopus 로고
    • G. Weber, Biochem. J. 1952, 51, 145-155.
    • (1952) Biochem. J , vol.51 , pp. 145-155
    • Weber, G.1
  • 11
    • 54349129033 scopus 로고    scopus 로고
    • The fluorescence lifetime (θ) is related to the molecular weight (M) in an approximation for globular molecules through the following equation: θ=ηM(v+h)/(RT), where R is the ideal gas constant, T the absolute temperature, η the viscosity, v the specific of the moiety, and h the hydration.
    • The fluorescence lifetime (θ) is related to the molecular weight (M) in an approximation for globular molecules through the following equation: θ=ηM(v+h)/(RT), where R is the ideal gas constant, T the absolute temperature, η the viscosity, v the specific volume of the moiety, and h the hydration.
  • 18
    • 54349099643 scopus 로고    scopus 로고
    • Perrin equation:p/1-3/1, po/1-3/1, 1+ηv/RTτo, where p is the polarization, po the value of p when no depolarization takes place, R the ideal gas constant, Τ the absolute temperature, η the viscosity of the solvent, V the molecular of the fluorescent molecule, and τo the fluorescence lifetime of the fluorophore
    • o the fluorescence lifetime of the fluorophore.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.