Effects of the Cdc2-like kinase-family and DNA topoisomerase I on the alternative splicing of eNOS in TNF-α-stimulated human endothelial cells

Biological Chemistry

Volumn 389, Issue 10, 2008, Pages 1333-1338

  Eisenreich, Andreas ^a   Boltzen, Ulrike ^a   Poller, Wolfgang ^a   Schultheiss, Heinz Peter ^a   Rauch, Ursula ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Camptothecin; Human umbilical vein endothelial cells (HUVECs); Inflammation; Nitric oxide; TG003

Indexed keywords

CDC2 LIKE KINASE; CYCLIN DEPENDENT KINASE 1; DNA TOPOISOMERASE; ENDOTHELIAL NITRIC OXIDE SYNTHASE; ISOENZYME; NITRIC OXIDE; PROTEIN SERINE THREONINE KINASE; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG;

ALTERNATIVE RNA SPLICING; ARTICLE; CELL LINE; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME INHIBITION; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; UMBILICAL VEIN;

ALTERNATIVE SPLICING; CDC2 PROTEIN KINASE; DNA TOPOISOMERASES, TYPE I; ENDOTHELIUM, VASCULAR; HUMANS; NITRIC OXIDE; NITRIC OXIDE SYNTHASE TYPE III; TUMOR NECROSIS FACTOR-ALPHA; UMBILICAL VEINS;

EID: 54049130815     PISSN: 14316730     EISSN: 14374315     Source Type: Journal    
DOI: 10.1515/BC.2008.152     Document Type: Article

References (30)

1. Baloch, Z., Cohen, S., and Coffman, F.D. (1990). Synergistic interactions between tumor necrosis factor and inhibitors of DNA topoisomerase I and II. J. Immunol. 145, 2908-2913.
2. Bogdanov, V.Y. (2006). Blood coagulation and alternative premRNA splicing: an overview. Curr. Mol. Med. 6, 859-869.
3. Bourgeois, C.F., Lejeune, F., and Stévenin, J. (2004). Broad specificity of SR (serine/arginine) proteins in the regulation of alternative splicing of pre-messenger RNA. Prog. Nucleic Acid Res. Mol. Biol. 78, 37-88.
4. Chao, J., Miao, R.O., Chen, V., Chen, L.M., and Chao, L. (2001). Novel roles of kallistatin, a specific tissue kallikrein inhibitor, in vascular remodeling. Biol. Chem. 382, 15-21.
5. Eisenreich, A., Celebi, Ö., Goldin-Lang, P., Schultheiss, H.P., and Rauch, U. (2008). Upregulation of tissue factor expression and thrombogenic activity in human aortic smooth muscle cells by irradiation, rapamycin and paclitaxel. Int. Immunopharmacol. 8, 307-311.
6. Förstermann, U. (2006). Janus-faced role of endothelial NO synthase in vascular disease: uncoupling of oxygen reduction from NO synthesis and its pharmacological reversal. Biol. Chem. 387, 1521-1533.
7. Goodwin, B., Pendleton, L., Levy, M., Solomonson, L., and Eichler, D. (2007). Tumor necrosis factor a reduces argininosuccinate synthase expression and nitric oxide production in aortic endothelial cells. Am. J. Physiol. Heart Circ. Physiol. 293, H1115-1121.
8. Heitzer, T., Schlinzig, T., Krohn, K., Meinertz, T., and Munzel, T. (2001). Endothelial dysfunction, oxidative stress, and risk of cardiovascular events in patients with coronary artery disease. Circulation 104, 2673-2678.
9. Hui, J. and Bindereif, A. (2005). Alternative pre-mRNA splicing in the human system: unexpected role of repetitive sequences as regulatory elements. Biol. Chem. 386, 1265-1271.
10. Lorenz, M., Hewing, B., Hui, J., Zepp, A., Baumann, G., Bindereif, A., Stangl, V., and Stangl, K. (2007). Alternative splicing in intron 13 of the human eNOS gene: a potential mechanism for regulating eNOS activity. FASEB J. 21, 1556-1564.
11. 2+ but requires phosphorylation by Akt at Ser(1179). J. Biol. Chem. 276, 30392-30398.
12. Muraki, M., Ohkawara, B., Hosoya, T., Onogi, H., Koizumi, J., Koizumi, T., Sumi, K., Yomoda, J., Murray, M.V., Kimura, H., et al. (2004). Manipulation of alternative splicing by a newly developed inhibitor of Clks. J. Biol. Chem. 279, 24246-24254.
13. Prasad, J. and Manley, J.L. (2003). Regulation and substrate specificity of the SR protein kinase Clk/Sty. Mol. Cell. Biol. 23, 4139-4149.
14. Prasad, J., Colwill, K., Pawson, T., and Manley, J.L. (1999). The protein kinase Clk/Sty directly modulates SR protein activity: both hyper- and hypophosphorylation inhibit splicing. Mol. Cell. Biol. 19, 6991-7000.
15. Rassaf, T., Kleinbongard, P., and Kelm, M. (2006). The L-arginine nitric oxide pathway: avenue for a multiple-level approach to assess vascular function. Biol. Chem. 387, 1347-1349.
16. Rauch, U. and Nemerson, Y. (2000). Circulating tissue factor and thrombosis. Curr. Opin. Hematol. 7, 273-277.
17. Rauch, U., Osende, J.I., Fuster, V., Badimon, J.J., Fayad, Z., and Chesebro, J.H. (2001). Thrombus formation on atherosclerotic plaques: pathogenesis and clinical consequences. Ann. Intern. Med. 134, 224-238.
18. Shen, B. and El-Dahr, S.S. (2006). Cross-talk of the renin-angiotensin and kallikrein-kinin systems. Biol. Chem. 387, 145-150.
19. Soret, J., Gabut, M., Dupon, C., Kohlhagen, G., Stévenin, J., Pommier, Y., and Tazi, J. (2003). Altered serine/arginine-rich protein phosphorylation and exonic enhancer-dependent splicing in Mammalian cells lacking topoisomerase I. Cancer Res. 63, 8203-8211.
20. Stellbaum, C., Willich, T., Boltzen, U., Ayral, Y., Szotowski, B., Piorkowski, M., Schultheiss, H.P., and Rauch, U. (2007). Clopidogrel-mediated reduction of circulating tissue factor in patients with stable coronary artery disease. Eur. J. Haematol. 78, 347-352.
21. Swintek, A.U., Christoph, S., Petrat, F., de Groot, H., and Kirsch, M. (2004). Cell type-dependent release of nitric oxide and/or reactive nitrogenoxide species from intracellular SIN-1: effects on cellular NAD(P)H. Biol. Chem. 385, 639-648.
22. Szotowski, B., Antoniak, S., Poller, W., Schultheiss, H.P., and Rauch, U. (2005a). Procoagulant soluble tissue factor is released from endothelial cells in response to inflammatory cytokines. Circ. Res. 96, 1233-1239.
23. Szotowski, B., Goldin-Lang, P., Antoniak, S., Bogdanov, V.Y., Pathirana, D., Pauschinger, M., Dörner, A., Kuehl, U., Coupland, S., Nemerson, Y., et al. (2005b). Alterations in myocardial tissue factor expression and cellular localization in dilated cardiomyopathy. J. Am. Coll. Cardiol. 45, 1081-1089.
24. Szotowski, B., Antoniak, S., and Rauch, U. (2006). Alternatively spliced tissue factor: a previously unknown piece in the puzzle of hemostasis. Trends Cardiovasc. Med. 16, 177-182.
25. Szotowski, B., Antoniak, S., Goldin-Lang, P., Tran, Q.V., Pels, K., Rosenthal, P., Bogdanov, V.Y., Borchert, H.H., Schultheiss, H.P., and Rauch, U. (2007). Antioxidative treatment inhibits the release of thrombogenic tissue factor from irradiation- and cytokine-induced endothelial cells. Cardiovasc. Res. 73, 806-812.
26. Tardos, J.G., Eisenreich, A., Deikus, G., Bechhofer, D.H., Chandradas, S., Zafar, U., Rauch, U., and Bogdanov, V.Y. (2008). SR proteins ASF/SF2 and SRp55 participate in tissue factor biosynthesis in human monocytic cells. J. Thromb. Haemost. 6, 877-884.
27. Utsugi, T., Mattern, M.R., Mirabelli, C.K., and Hanna, N. (1990). Potentiation of topoisomerase inhibitor-induced DNA strand breakage and cytotoxicity by tumor necrosis factor: enhancement of topoisomerase activity as a mechanism of potentiation. Cancer Res. 50, 2636-2640.
28. Velazquez-Dones, A., Hagopian, J.C., Ma, C.T., Zhong, X.Y., Zhou, H., Ghosh, G., Fu, X.D., and Adams, J.A. (2005). Mass spectrometric and kinetic analysis of ASF/SF2 phosphorylation by SRPK1 and Clk/Sty. J. Biol. Chem. 280, 41761-41768.
29. Xia, Z., Liu, M., Wu, Y., Sharma, V., Luo, T., Ouyang, J., and McNeill, J.H. (2006). N-acetylcysteine attenuates TNF-α-induced human vascular endothelial cell apoptosis and restores eNOS expression. Eur. J. Pharmacol. 550, 134-142.
30. Ying, L. and Hofseth, L.J. (2007). An emerging role for endothelial nitric oxide synthase in chronic inflammation and cancer. Cancer Res. 67, 1407-1410.