Chemical shift based editing of CH3 groups in fractionally 13C-labelled proteins using GFT (3, 2)D CT-HCCH-COSY: Stereospecific assignments of CH3 groups of Val and Leu residues

Journal of Biomolecular NMR

Volumn 42, Issue 2, 2008, Pages 149-154

  Barnwal, Ravi Pratap ^a   Atreya, Hanudatta S ^a   Chary, Kandala V R ^b  

^a TATA INSTITUTE OF FUNDAMENTAL RESEARCH   (India)

^b INDIAN INSTITUTE OF SCIENCE   (India)

Author keywords

Automated assignments; Isotope labeling; NMR; Sequence specific resonance assignments; Stereo specific resonance assignments

Indexed keywords

ALANINE; CARBON 13; ISOLEUCINE; LEUCINE; METHIONINE; METHYL GROUP; THREONINE; VALINE;

AMINO ACID SEQUENCE; ARTICLE; FOURIER ANALYSIS; ISOTOPE LABELING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; SIMULATION; STEREOSPECIFICITY; STRUCTURE ANALYSIS;

CARBON ISOTOPES; CRYSTALLINS; LEUCINE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEINS; VALINE;

EID: 54049117925     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-008-9273-z     Document Type: Article

References (26)

1. Atreya HS, Chary KVR (2001) Selective 'unlabeling' of amino acids in fractionally 13C labeled proteins: An approach for stereospecific NMR assignments of CH3 groups in Val and Leu residues. J Biomol NMR 19:267-272
2. Atreya HS, Szyperski T (2004) G-matrix Fourier transform NMR spectroscopy for complete protein resonance assignment. Proc Natl Acad Sci USA 101:9642-9647
3. Atreya HS, Szyperski T (2005) Rapid NMR data collection. Meth Enzymol 394:78-108
4. Atreya HS, Sahu SC, Bhattacharya A, Chary KVR, Govil G (2001) NMR derived solution structure of an EF-hand calcium-binding protein from Entamoeba histolytica. Biochemistry 40:14392-14403
5. 2+ binding protein of the betagamma-crystallin superfamily from Methanosarcina acetivorans. J Biomol NMR 36 Suppl:1-32
6. Barnwal RP, Rout AK, Chary KVR, Atreya HS (2007) Rapid measurement of 3 J(H N-H alpha) and 3 J(N-H beta) coupling constants in polypeptides. J Biomol NMR 39:259-263
7. Barnwal RP, Rout AK, Atreya HS, Chary KVR (2008a) Identification of C-terminal neighbours of amino acid residues without an aliphatic (13)C (gamma) as an aid to NMR assignments in proteins. J Biomol NMR 41(4):191-197
8. Barnwal RP, Rout AK, Chary KVR, Atreya HS (2008b) Rapid measurement of Pseudocontact shifts in paramagnetic proteins by GFT NMR Spectroscopy. Open Magn Reson J (open access) 1:16-28
9. Bartels C, Xia TH, Billeter M, Guntert P, Wüthrich K (1995) The program XEASY for computer-supported NMR spectral analysis of biological macromolecules. J Biomol NMR 6:1-10
10. Chary KVR, Govil G (2008) NMR in biological systems: From molecules to humans. Springer, The Netherlands, pp 1-550
11. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: A multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6:277-293
12. 15N multidimensional NMR to study the structure and dynamics of proteins. Annu Rev Biophys Biomol Struct 27:357-406
13. Hu W, Zuiderweg ER (1996) Stereospecific assignments of Val and Leu methyl groups in a selectively 13C-labeled 18 kDa polypeptide using 3D CT-(H) CCH-COSY and 2d 1Jc-c edited heteronuclear correlation experiments. J Magn Reson B 113:70-75
14. Kay LE, Keifer P, Saarinen T (1992) Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity. J Am Chem Soc 114:10663-10665
15. Keller R (2004) The computer aided resonance assignment tutorial. Verlag Goldau
16. Kim S, Szyperski T (2003) GFT NMR, a new approach to rapidly obtain precise high-dimensional NMR spectral information. J Am Chem Soc 125:1385-1393
17. Neri D, Szyperski T, Otting G, Senn H, Wüthrich K (1989) Stereospecific nuclear magnetic resonance assignments of the methyl groups of valine and leucine in the DNA-binding domain of the 434 repressor by biosynthetically directed fractional 13C labeling. Biochemistry 28:7510-7516
18. Ostler G, Soterious A, Moody CM, Khan JA, Birsall B, Carr MD, Young DW, Feeney J (1993) Stereospecific assignments of the leucine methyl resonances in the 1H NMR spectrum of Lactobacillus casei dihydrofolate reductase. FEBS Lett 318:177-180
19. Pervushin K (2000) Impact of transverse relaxation optimized spectroscopy (TROSY) on NMR as a technique in structural biology. Q Rev Biophys 33:161-197
20. Sattler M, Schwalbe H, Griesinger C (1992) Stereospecific assignment of leucine methyl groups with carbon-13 in natural abundance or with random 13C labeling. J Am Chem Soc 114:1126-1127
21. Shaka AJ, Barker PB, Freeman R (1985) Computer-optimized decoupling scheme for wideband applications and low-level operation. J Magn Reson 64:547-552
22. Szyperski T, Atreya HS (2006) Principles and applications of GFT projection NMR spectroscopy. Magn Reson Chem 44:S51-S60
23. Tate S, Ushioda T, Utsunomiya-Tate N, Shibuya K, Ohyama Y, Nakano Y, Kaji H, Inagaki F, Samejima T, Kainosho M (1995) Solution structure of a human cystatin A variant, cystatin A2-98 M65L, by NMR spectroscopy. A possible role of the interactions between the N- and C-termini to maintain the inhibitory active form of cystatin A. Biochemistry 34:14637-14648
24. Tugarinov V, Kay LE (2003) Ile, Leu, and Val methyl assignments of the 723-residue malate synthase G using a new labeling strategy and novel NMR methods. J Am Chem Soc 125:13868-13878
25. Vuister GW, Wang AC, Bax A (1993) Measurement of three-bond nitrogen-carbon J couplings in proteins uniformly enriched in nitrogen-15 and carbon-13. J Am Chem Soc 115:5334-5335
26. Wüthrich K (1986) NMR of proteins and nucleic acids. Wiley Publishers, New-York, pp 1-292