An O-phosphotransferase catalyzes phosphorylation of hygromycin A in the antibiotic-producing organism Streptomyces hygroscopicus

Antimicrobial Agents and Chemotherapy

Volumn 52, Issue 10, 2008, Pages 3580-3588

  Dhote, Vidya ^a   Gupta, Shuchi ^a   Reynolds, Kevin A ^a  

^a PORTLAND STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

5'' DIHYDRODESMETHYLENEHYGROMYCIN A; 5'' DIHYDROHYGROMYCIN; 5'' DIHYDROMETHOXYHYGROMYCIN A; ADENOSINE TRIPHOSPHATE; ANTIBIOTIC A201A; ANTIBIOTIC AGENT; DESMETHYLENEHYGROMYCIN A; DNA FRAGMENT; GUANOSINE DIPHOSPHATE; HYGROMYCIN; METHOXYHYGROMYCIN A; PHOSPHOTRANSFERASE; PLASMID DNA; PUROMYCIN; TRITIUM; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM CULTURE; BIOASSAY; BIOSYNTHESIS; CATALYSIS; COLONY FORMING UNIT; ENZYME PHOSPHORYLATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; FERMENTATION; GENE OVEREXPRESSION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; MASS SPECTROMETRY; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS INHIBITION; PROTON NUCLEAR MAGNETIC RESONANCE; STREPTOMYCES HYGROSCOPICUS;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BASE SEQUENCE; CINNAMATES; CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; HYGROMYCIN B; KINETICS; MUTATION; PHOSPHORYLATION; PHOSPHOTRANSFERASES; RECOMBINANT PROTEINS; STREPTOMYCES; SUBSTRATE SPECIFICITY;

EID: 54049104407     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.00157-08     Document Type: Article

References (25)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Barrasa, M. I., J. A. Tercero, and A. Jimenez. 1997. The aminonucleoside antibiotic A201A is inactivated by a phosphotransferase activity from Streptomyces capreolus NRRL 3817, the producing organism: isolation and molecular characterization of the relevant encoding gene and its DNA flanking regions. Eur. J. Biochem. 245:54-63.
3. Butler, A. R., S. A. Flint, and E. Cundliffe. 2001. Feedback control of polyketide metabolism during tylosin production. Microbiology 147:795-801.
4. Cundliffe, E. 1989. How antibiotic-producing organisms avoid suicide. Annu. Rev. Microbiol. 43:207-233.
5. Fralick, J. A. 1996. Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli. J. Bacteriol. 178:5803-5805.
6. Gourmelen, A., M. H. Blondelet-Rouault, and J. L. Pernodet. 1998. Characterization of a glycosyl transferase inactivating macrolides, encoded by gimA from Streptomyces ambofaciens. Antimicrob. Agents Chemother. 42:2612-2619.
7. Guerrero, M. D., and J. Modolell. 1980. Hygromycin A, a novel inhibitor of ribosomal peptidyltransferase. Eur. J. Biochem. 107:409-414.
8. Gust, B., G. L. Challis, K. Fowler, T. Kieser, and K. F. Chater. 2003. PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin. Proc. Natl. Acad. Sci. USA 100:1541-1546.
9. Habib el, S. E., J. N. Scarsdale, and K. A. Reynolds. 2003. Biosynthetic origin of hygromycin A. Antimicrob. Agents Chemother. 47:2065-2071.
10. Hawkey, P. M. 1998. The origins and molecular basis of antibiotic resistance. BMJ 317:657-660.
11. Hopwood, D. A. 2007. How do antibiotic-producing bacteria ensure their self-resistance before antibiotic biosynthesis incapacitates them? Mol. Microbiol. 63:937-940.
12. Hulo, N., A. Bairoch, V. Bulliard, L. Cerutti, E. De Castro, P. S. Langendijk-Genevaux, M. Pagni, and C. J. Sigrist. 2006. The PROSITE database. Nucleic Acids Res. 34:D227-230.
13. Mansouri, K., and W. Piepersberg. 1991. Genetics of streptomycin production in Streptomyces griseus: nucleotide sequence of five genes, strFGHIK, including a phosphatase gene. Mol. Gen. Genet. 228:459-469.
14. Martin, J. F., and P. Liras. 1989. Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites. Annu. Rev. Microbiol. 43:173-206.
15. Miller, A. L., and J. B. Walker. 1970. Accumulation of streptomycin-phosphate in cultures of streptomycin producers grown on a high-phosphate medium. J. Bacteriol. 104:8-12.
16. Palaniappan, N., S. Ayers, S. Gupta, S. Habib el, and K. A. Reynolds. 2006. Production of hygromycin A analogs in Streptomyces hygroscopicus NRRL 2388 through identification and manipulation of the biosynthetic gene cluster. Chem. Biol. 13:753-764.
17. Perez-Gonzalez, J. A., M. Lopez-Cabrera, J. M. Pardo, and A. Jimenez. 1989. Biochemical characterization of two cloned resistance determinants encoding a paromomycin acetyltransferase and a paromomycin phosphotransferase from Streptomyces rimosus forma paromomycinus. J. Bacteriol. 171:329-334.
18. Saraste, M., P. R. Sibbald, and A. Wittinghofer. 1990. The P-loop: a common motif in ATP- and GTP-binding proteins. Trends Biochem. Sci. 15:430-434.
19. Smith, C. A., and E. N. Baker. 2002. Aminoglycoside antibiotic resistance by enzymatic deactivation. Curr. Drug Targets Infect. Disord. 2:143-160.
20. Thompson, C. J., R. H. Skinner, J. Thompson, J. M. Ward, D. A. Hopwood, and E. Cundliffe. 1982. Biochemical characterization of resistance determinants cloned from antibiotic-producing streptomycetes. J. Bacteriol. 151:678-685.
21. Vilches, C., C. Hernandez, C. Mendez, and J. A. Salas. 1992. Role of glycosylation and deglycosylation in biosynthesis of and resistance to oleandomycin in the producer organism, Streptomyces antibioticus. J. Bacteriol. 174:161-165.
22. Walker, J. B., and M. S. Walker. 1967. Streptomycin biosynthesis. Enzymatic synthesis of O-phosphorylstreptidine from streptidine and adenosinetriphosphate. Biochim. Biophys. Acta 148:335-341.
23. Walker, J. E., M. Saraste, M. J. Runswick, and N. J. Gay. 1982. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1:945-951.
24. Wright, G. D., and P. R. Thompson. 1999. Aminoglycoside phosphotransferases: proteins, structure, and mechanism. Front. Biosci. 4:D9-D21.
25. Zhao, L., N. J. Beyer, S. A. Borisova, and H. W. Liu. 2003. Beta-glucosylation as a part of self-resistance mechanism in the methymycin/pikromycin producing strain Streptomyces venezuelae. Biochemistry 42:14794-14804.