Synergy between ajoene and ketoconazole in isolates of Microsporum canis. A preliminary study using fractional inhibitory concentration technique (FIC);Sinergismo entre ajoeno y ketoconazol en aislamientos de Microsporum canis. Un estudio preliminar mediante la determinación de la concentración inhibitoria fraccional (CIF)

Revista Iberoamericana de Micologia

Volumn 25, Issue 3, 2008, Pages 157-162

  Ledezma, Eliades ^a   Maniscalchi, Maria Teresa ^b   Espinoza, Druvic Lemus ^b  

^a Universidad de Oriente ^*  (Venezuela)

^b UNIVERSIDAD DE ORIENTE   (Venezuela)

Author keywords

Ajoene; Antifungal; Dermatophytes; Ketoconazole; Microsporum canis; Tinea capitis

Indexed keywords

AJOENE; KETOCONAZOLE;

ANALYTIC METHOD; ANTIFUNGAL ACTIVITY; ARTICLE; BROTH DILUTION; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG ANTAGONISM; DRUG EFFICACY; DRUG POTENCY; DRUG POTENTIATION; FRACTIONAL INHIBITORY CONCENTRATION TECHNIQUE; FUNGAL VIRULENCE; FUNGUS GROWTH; FUNGUS ISOLATION; MICROSPORUM CANIS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN;

ARTHRODERMATACEAE; FUNGI; MICROSPORUM CANIS;

EID: 53849142599     PISSN: 11301406     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1130-1406(08)70037-8     Document Type: Article

References (27)

1. Alió A, Mendoza M, Zambrano E, Diaz E, Cavallera E. Susceptibility and resistance testing in vitro of dermatophytes with broth microdilution method guided by their growth curve. Mycoses 2001; 44: 3-83 (S).
2. Apitz-Castro R, Cabrera S, Cruz M, Ledezma E, Jain M. Effects of garlic extract and of three pure components isolated from it on human platelet aggregation, arachidonate metabolims, release reaction and platelet ultrastructure. Thromb Res 1983; 32: 155-169.
3. Apitz-Castro R, Ledezma E, Escalante J, Jorquera A, Piñate F, Moreno-Rea J. Carrilo G, Leal O, Jain M K. Reversible prevention of platelet activation by (E,Z)-4,5,9-trithiadodeca-1,6,11-treine 9-oxide (ajoene) in dogs under extracorporeal circulation. Arzneimittelforschung 1988; 38: 901-904.
4. Apitz-Castro R, Escalante J, Vargas R, Jain M K. Ajoene, the antiplatelet principle of garlic, synergistically potentiales the antiaggregatory action of prostacyclin, forskolin, indomethacin acid, dypiridamol on human platelets. Thromb Res 1986; 42: 303-311
5. EUCAST-AFST. European Committee on Antibiotic Susceptibility Testing. Subcommittee of Antifungal Susceptibility Testing. Method for the determination of minimum inhibitory concentration (MIC) by broth dilution of fermentative yeasts. European Society of Clinical Microbiology and Infectious Diseases, Germany, 2002.
6. Guarro U, Llop C, Aguilar C, Pujol I. Comparison of in vitro antifungal susceptibilities of conidia and hyphae of filamentous fungi. Antimicrob Agents Chemother 1997; 41: 2760-2762.
7. Gupta A K, Kohli Y. In vitro susceptibility testing of ciclopirox, terbinafine, ketoconazole and itraconazole against dermatophytes and nondermatophytes, and in vitro evaluation of combination antifungal activity. Br J Dermatol 2003; 149: 296-305.
8. Ledezma E, Apitz-Castro R. Ajoene, el principal compuesto activo derivado del ajo (Allium sativum), un nuevo agente antifúngico. Rev Iberoam Micol 2006; 23: 75-80.
9. Ledezma E, De Sousa, L, Jorquera A, Sanchez J, Lander A, Rodriguez E, Apitz-Castro R, Jain M K. Efficacy of ajoene, an organosulphur derived from garlic in the short-term therapy of tinea pedis. Mycoses 1996; 39: 393-395.
10. Ledezma E, López J C, Marin P, Romero H, Ferrara G, De Sousa L, Jorquera A, Apitz-Castro R. Ajoene in the topical short-term treatment of tinea cruris y tines corporis in humans. Randomized comparative study with terbinafine. Drug Research 1999; 49: 544-547.
11. Ledezma E, Marcano K, Jorquera A, De Sousa L, Padilla M, Pulgar M, Apitz-Castro R. Efficacy of ajoene in the treatment of tinea pedis: a doble-blind and comparative study with terbinafine. J Am Acad Dermatol 2000; 43: 829-832.
12. Ledezma E, Apitz-Castro R. Del folklore al mecanismo molecular: el ejemplo del ajoene. Interciencia 1998; 23: 227-231.
13. Llop C, Pujol I, Aguilar C, Sala J, Riba D, Guarro J. Comparison of three methods of determining MICs for filamentous fungi using different end point criteria and incubation periods. Antimicrob Agents Chemother 2000; 44: 239-242.
14. Maniscalchi M T, Lemus D, Ledezma E, Sánchez J, Vivas J, Apitz-Castro R. Estudio de la susceptibilidad in vitro de aislados de Microsporum canis al ajoene, terbinafina y griseofulvina, utilizando el método de microdilución. Rev Soc Ven Microbiol 2004; 24: 40-45.
15. Mosquera J, Sharp A, Moore C B, Warn P A, Denning D W. In vitro interaction of terbinafine with itraconazole, fluconazole, amphotericin B and 5-flucytosine against Aspergillus spp. J Antimicrob Chemother. 2002; 50: 189-194
16. NCCLS. National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi. Approved standard M38-A, Wayne, Pa. 2002; Vol. 22.
17. Pérez G, Ruiz E, Vivas J. Efectos antiproliferativos de ketoconazol y ajoene en promastigotes de Leishmania mexicans. Efectos sinérgicos. XV Congreso Latinoamericano de Parasitología, São Paulo, Brasil, octubre de 2001.
18. Ramírez C, Ruiz E, Vivas J. Efectos antiproliferativos de ketoconazol y ajoene en epimastigotes de Trypanosoma cruzi. Efectos sinérgicos. XV Congreso Latinoamericano de Parasitología, São Paulo, Brasil, octubre de 2001.
19. Rojas G C, Vivas J, Guerrero O. Estandarización del inóculo de Cladophialophora carrionii por el método espectrofotométrico para estudios de sensibilidad in vitro de hongos filamentosos. Rev Soc Ven Microbiol 2004; 24: 89-94.
20. Romero H, Vivas J, Chalbaud V, Ledezma E, Apitz-Castro R. In vitro antiproliferative effect of ajoene on Microsporum canis. J Mycol Med 2000; 10: 152-155.
21. San Blas G, Urbina U, Marchán E, Contreras L, Sorais F, San Blas F. Inhibition of Paracoccidioides brasiliensis by ajoene is associated wich blockade of phosphatidylcholine biosynthesis. Microbiology 1997; 143: 1583-1586.
22. Sud I J, Feingold D S. Effect of Ketoconazole in combination with other inhibitors of sterol synthesis on fungal growth. Antimicrob Agents Chemother 1985; 28: 532-534
23. Tallarida RJ. Drug synergism: its detection and applications. Perspectives in pharmacology. J Pharmacol Exp Ther 2001; 298: 865-872.
24. Vazquez JA. Combination antifungal therapy against Candida species: the new frontier - are we there yet? Med Mycol 2003; 41: 355-368.
25. Vitale RG, Afeltra J, Dannaoui E. Antifungal combinations. Methods Mol Med 2005; 118: 143-152.
26. Yoshida S, Shigeo K, Norihiro H, Tsuyoshi U, Hiromichi M, Shizutoshi N. Antifungal activity of ajoene derived from garlic. Appl Environ Microbiol 1987; 53: 615-617.
27. Zhang L, Yan K, Zhang Y, Huang R, Bian J, Zheng C, Sun H, Chen Z, Sun N, An R, Min F, Zhao W, Zhuo Y, You J, Song Y, Yu Z, Liu Z, Yang K, Gao H, Dai H, Zhang X, Wang J, Fu C, Pei G, Liu J, Zhang S, Goodfellow M, Jiang Y, Kuai J, Zhou G, Chen X. High-throughput synergy screening identifies microbial metabolites as combination agents for the treatment of fungal infections. Proc Natl Acad Sci USA 2007 13; 104: 4606-4611.