Ochratoxin a in laying hens: High-performance liquid chromatography detection and cytological and histological analysis of target tissues

Journal of Applied Poultry Research

Volumn 17, Issue 1, 2008, Pages 151-156

  Bozzo, G ^a   Ceci, E ^a   Bonerba, E ^a   Desantis, S ^a   Tantillo, G ^a  

^a UNIVERSITY OF BARI   (Italy)

Author keywords

High performance liquid chromatography; Kidney; Liver; Ochratoxin a

Indexed keywords

ANIMALIA; RATTUS;

EID: 53649099964     PISSN: 10566171     EISSN: None     Source Type: Journal    
DOI: 10.3382/japr.2007-00058     Document Type: Article

References (22)

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8. Supelco ochratoxin (product no. 46912) [9], packaged in sealed ampoules at a concentration of about 50 ng/ μL in benzene:acetic acid (99:1), was used as standard. This standard was prepared according to the AOAC official methods [10]. The standards for the OchraTest by HPLC were prepared as follows: a) ochratoxin-working solution 1: dilution 1:50 (in methanol) of Supelco ochratoxin A standard (50 ng/μL) and b) ochratoxin-working solution 2: diluting ochratoxin-working solution 1 (1 ng/μL) 10 times with methanol. The calibration curve was performed diluting ochratoxin-working solution 2 with methanol in order to obtain the following concentrations: 1.0, 10.0, 20.0, and 50.0 (ng/g). We added 1.5 mL of water to all our standards and samples before injecting onto the HPLC to make the solvent for the standards and samples similar to the mobile phase.
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