Molecular-beacon-based real-time PCR for detection and quantification of Mycobacterium tuberculosis DNA in clinical samples

Journal of Clinical Microbiology

Volumn 46, Issue 9, 2008, Pages 3177-3178

  Papaparaskevas, Joseph ^a   Houhoula, Dimitra P ^a   Siatelis, Argirios ^a   Tsakris, Athanassios ^a  

^a UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; COMPUTER PROGRAM; HUMAN; LETTER; LUNG TUBERCULOSIS; LYMPH NODE BIOPSY; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

DNA, BACTERIAL; HUMANS; MYCOBACTERIUM TUBERCULOSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TUBERCULOSIS, PULMONARY;

MYCOBACTERIUM TUBERCULOSIS;

EID: 53649099632     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00903-08     Document Type: Letter

References (11)

1. Broccolo, F., P. Scarpellini, G. Locatelli, A. Zingale, A. M. Brambilla, P. Cichero, L. A. Sechi, A. Lazzarin, P. Lusso, and M. S. Malnati. 2003. Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays. J. Clin. Microbiol. 41:4565-4572.
2. Bruijnesteijn van Coppenraet, E. S., J. A. Lindeboom, J. M. Prins, M. F. Peeters, E. C. J. Claas, and E. J. Kuijper. 2004. Real-time PCR assay using fine-needle aspirates and tissue biopsy specimens for rapid diagnosis of mycobacterial lymphadenitis in children. J. Clin. Microbiol. 42:2644-2650.
3. Cleary, T. J., G. Roudel, O. Casillas, and N. Miller. 2003. Rapid and specific detection of Mycobacterium tuberculosis by using the Smart Cycler instrument and a specific fluorogenic probe. J. Clin. Microbiol. 41:4783-4786.
4. Delta-Latta, P. 2004. Mycobacteriology and antimycobacterial susceptibility testing, p. 7.1.1-7.3.1. In H. D. Isenberg (ed.), Clinical microbiology procedures handbook, 2nd ed. ASM Press, Washington, DC.
5. Espy, M. J., J. R. Uhl, L. M. Sloan, S. P. Buckwalter, M. F. Jones, E. A. Vetter, J. D. C. Yao, N. L. Wengenack, J. E. Rosenblatt, F. R. Cockerill III, and T. F. Smith. 2006. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin. Microbiol. Rev. 19:165-256.
6. Fang, Y., W. H. Wu, J. L. Pepper, J. L. Larsen, S. A. E. Marras, E. A. Nelson, W. B. Epperson, and J. Christopher-Hennings. 2002. Comparison of realtime, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples. J. Clin. Microbiol. 40:287-291.
7. Haldar, S., S. Chakravorty, M. Bhalla, S. De Majumdar, and J. S. Tyagi. 2007. Simplified detection of Mycobacterium tuberculosis in sputum using smear microscopy and PCR with molecular beacons. J. Med. Microbiol. 56:1356-1362.
8. Kox, L. F. F., D. Rhienthong, A. Medo-Miranda, N. Udomsantisuk, K. Ellis, J. Van Leeuwen, S. Van Heusden, S. Kuijper, and A. H. J. Kolk. 1994. A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples. J. Clin. Microbiol. 32:672-678.
9. Piatek, A. S., A. Telenti, M. R. Murray, H. El-Hajj, W. R. Jacobs, Jr., F. R. Kramer, and D. Alland. 2000. Genotypic analysis of Mycobacterium tuberculosis in two distinct populations using molecular beacons: implications for rapid susceptibility testing. Antimicrob. Agents Chemother. 44:103-110.
10. Varma-Basil, M., H. El-Hajj, R. Colangeli, M. H. Hazbon, S. Kumar, M. Bose, M. Bobadilla-del-Valle, L. G. Garcia, A. Hernandez, F. R. Kramer, J. S. Osornio, A. Ponce-de-Leon, and D. Alland. 2004. Rapid detection of rifampin resistance in Mycobacterium tuberculosis isolates from India and Mexico by a molecular beacon assay. J. Clin. Microbiol. 42:5512-5516.
11. World Health Organization. 2003. Treatment of tuberculosis: guidelines for national programmes, 3rd ed. WHO/CDS/TB/2003.313. World Health Organization, Geneva, Switzerland. http://www.who.int/tb/publications/ cds_tb_2003_313/en/index.html. Accessed 7 May 2008.