Development and validation of a quantitative PCR assay for diagnosis of scedosporiosis

Journal of Clinical Microbiology

Volumn 46, Issue 10, 2008, Pages 3412-3416

  Castelli, Maria V ^a   Buitrago, Maria J ^a   Bernal Martinez, Leticia ^a   Gomez Lopez, Alicia ^a   Rodriguez Tudela, Juan L ^a   Cuenca Estrella, Manuel ^a  

^a INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSOME DNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DNA DETERMINATION; LUNG PARENCHYMA; MOUSE; MYCOSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCEDOSPORIOSIS; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY;

ANIMALS; BLOOD; DNA, FUNGAL; FLUORESCENT DYES; HUMANS; LUNG; MALE; MICE; MYCOSES; OLIGONUCLEOTIDE PROBES; POLYMERASE CHAIN REACTION; SCEDOSPORIUM; SENSITIVITY AND SPECIFICITY; SURVIVAL ANALYSIS;

MURINAE; MUS; PSEUDALLESCHERIA BOYDII; SCEDOSPORIUM PROLIFICANS;

EID: 53649096949     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00046-08     Document Type: Article

References (22)

1. Bagyalakshmi, R., K. L. Therese, S. Prasanna, and H. N. Madhavan. 2008. Newer emerging pathogens of ocular nonsporulating molds (NSM) identified by polymerase chain reaction (PCR)-based DNA sequencing technique targeting internal transcribed spacer (ITS) region. Curr. Eye Res. 33:139-147.
2. Berenguer, J., J. L. Rodriguez-Tudela, C. Richard, M. Alvarez, M. A. Sanz, L. Gaztelurrutia, J. Ayats, J. V. Martinez-Suarez, et al. 1997. Deep infections caused by Scedosporium prolificans. A report on 16 cases in Spain and a review of the literature. Medicine (Baltimore) 76:256-265.
3. Cano, J., J. Guarro, E. Mayayo, and J. Fernandez-Ballart. 1992. Experimental infection with Scedosporium inflatum. J. Med. Vet. Mycol. 30:413-420.
4. Capilla, J., C. Serena, F. J. Pastor, M. Ortoneda, and J. Guarro. 2003. Efficacy of voriconazole in treatment of systemic scedosporiosis in neutropenic mice. Antimicrob. Agents Chemother. 47:3976-3978.
5. Cuenca-Estrella, M., A. Gomez-Lopez, E. Mellado, M. J. Buitrago, A. Monzon, and J. L. Rodriguez-Tudela. 2006. Head-to-head comparison of the activities of currently available antifungal agents against 3,378 Spanish clinical isolates of yeasts and filamentous fungi. Antimicrob. Agents Chemother. 50:917-921.
6. Cuenca-Estrella, M., B. Ruiz-Diez, J. V. Martinez-Suarez, A. Monzon, and J. L. Rodriguez-Tudela. 1999. Comparative in-vitro activity of voriconazole (UK-109,496) and six other antifungal agents against clinical isolates of Scedosporium prolificans and Scedosporium apiospermum. J. Antimicrob. Chemother. 43:149-151.
7. de Batlle, J., M. Motje, R. Balanza, R. Guardia, and R. Ortiz. 2000. Disseminated infection caused by Scedosporium prolificans in a patient with acute multilineal leukemia. J. Clin. Microbiol. 38:1694-1695.
8. Gonzalez, G. M., R. Tijerina, L. Najvar, M. Rinaldi, I. T. Yeh, and J. R. Graybill. 2002. Experimental murine model of disseminated Pseudallescheria infection. Med. Mycol. 40:243-248.
9. Guarro, J., and J. Gene. 2002. Acrophialophora fusispora misidentified as Scedosporium prolificans. J. Clin. Microbiol. 40:3544.
10. Guarro, J., A. S. Kantarcioglu, R. Horre, J. L. Rodriguez-Tudela, E. M. Cuenca, J. Berenguer, and G. S. de Hoog. 2006. Scedosporium apiospermum: changing clinical spectrum of a therapy-refractory opportunist. Med. Mycol. 44:295-327.
11. Hagari, Y., S. Ishioka, F. Ohyama, and M. Mihara. 2002. Cutaneous infection showing sporotrichoid spread caused by Pseudallescheria boydii (Scedosporium apiospermum): successful detection of fungal DNA in formalin-fixed, paraffin-embedded sections by seminested PCR. Arch. Dermatol. 138: 271-272.
12. Hayden, R. T., P. A. Isotalo, T. Parrett, D. M. Wolk, X. Qian, G. D. Roberts, and R. V. Lloyd. 2003. In situ hybridization for the differentiation of Aspergillus, Fusarium, and Pseudallescheria species in tissue section. Diagn. Mol. Pathol. 12:21-26.
13. Jabado, N., J. L. Casanova, E. Haddad, F. Dulieu, J. C. Fournet, B. Dupont, A. Fischer, C. Hennequin, and S. Blanche. 1998. Invasive pulmonary infection due to Scedosporium apiospermum in two children with chronic granulomatous disease. Clin. Infect. Dis. 27:1437-1441.
14. Lau, A., S. Chen, T. Sorrell, D. Carter, R. Malik, P. Martin, and C. Halliday. 2007. Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. J. Clin. Microbiol. 45:380-385.
15. Pinto, M. R., B. Mulloy, R. M. Haido, L. R. Travassos, and B. E. Barreto. 2001. A peptidorhamnomannan from the mycelium of Pseudallescheria boydii is a potential diagnostic antigen of this emerging human pathogen. Microbiology 147:1499-1506.
16. Ponton, J., R. Ruchel, K. V. Clemons, D. C. Coleman, R. Grillot, J. Guarro, D. Aldebert, P. Ambroise-Thomas, J. Cano, A. J. Carrillo-Munoz, J. Gene, C. Pinel, D. A. Stevens, and D. J. Sullivan. 2000. Emerging pathogens. Med. Mycol. 38(Suppl. 1):225-236.
17. Rippon, J. W. 1988. Pseudallescheriasis, p. 651-680. In J. W. Rippon (ed.), Medical mycology: the pathogenic fungi and the pathogenic actinomycetes. W. B. Saunders Co., Philadelphia, PA.
18. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Smith, J. M., C. M. Tang, S. Van Noorden, and D. W. Holden. 1994. Virulence of Aspergillus fumigatus double mutants lacking restriction and an alkaline protease in a low-dose model of invasive pulmonary aspergillosis. Infect. Immun. 62:5247-5254.
20. Spiess, B., W. Seifarth, M. Hummel, O. Frank, A. Fabarius, C. Zheng, H. Morz, R. Hehlmann, and D. Buchheidt. 2007. DNA microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. J. Clin. Microbiol. 45:3743-3753.
21. Tang, C. M., J. Cohen, and D. W. Holden. 1992. An Aspergillus-fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity. Mol. Microbiol. 6:1663-1671.
22. Wedde, M., D. Muller, K. Tintelnot, G. S. de Hoog, and U. Stahl. 1998. PCR-based identification of clinically relevant Pseudallescheria/ Scedosporium strains. Med. Mycol. 36:61-67.