Rapid and inexpensive detection of multidrug-resistant Mycobacterium tuberculosis with the nitrate reductase assay using liquid medium and direct application to sputum samples

Journal of Clinical Microbiology

Volumn 46, Issue 10, 2008, Pages 3243-3245

  Affolabi, Dissou ^a,b   Odoun, Mathieu ^a   Sanoussi, N'dira ^a   Martin, Anandi ^b,c   Palomino, Juan Carlos ^b   Kestens, Luc ^b   Anagonou, Séverin ^a   Portaels, Françoise ^b  

^a Laboratoire de Référence des Mycobactéries   (Benin)

^b INSTITUTE OF TROPICAL MEDICINE   (Belgium)

^c MÉDECINS SANS FRONTIÈRES   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ISONIAZID; NITRATE REDUCTASE; RIFAMPICIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; ENZYME ASSAY; LIQUID CULTURE; MULTIDRUG RESISTANCE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; SPUTUM ANALYSIS;

BACTERIOLOGICAL TECHNIQUES; CULTURE MEDIA; HUMANS; MYCOBACTERIUM TUBERCULOSIS; NITRATE REDUCTASE; SENSITIVITY AND SPECIFICITY; SPUTUM; TUBERCULOSIS, MULTIDRUG-RESISTANT;

MYCOBACTERIUM TUBERCULOSIS;

EID: 53649095019     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00083-08     Document Type: Article

References (24)

1. Abate, G., A. Aseffa, A. Selassie, S. Goshu, B. Fekade, D. WoldeMeskal, and H. Miörner. 2004. Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 42:871-873.
2. Affolabi, D., M. Odoun, A. Martin, J. C. Palomino, S. Anagonou, and F. Portaels. 2007. Evaluation of direct detection of Mycobacterium tuberculosis rifampin resistance by a nitrate reductase assay applied to sputum samples in Cotonou, Benin. J. Clin. Microbiol. 45:2123-2125.
3. Altman, D. G. 1999. Practical statistics for medical research. Chapman & Hall/CRC, London, United Kingdom.
4. Angeby, K. A., L. Klintz, and S. E. Hoffner. 2002. Rapid and inexpensive drug susceptibility testing of Mycobacterium tuberculosis with a nitrate reductase assay. J. Clin. Microbiol. 40:553-555.
5. Aziz, M. A., A. Wright, A. Laszlo, A. De Muynck, F. Portaels, A. Van Deun, C. Wells, P. Nunn, L. Blanc, M. Raviglione, and WHO/International Union Against Tuberculosis And Lung Disease Global Project on Anti-Tuberculosis Drug Resistance Surveillance. 2006. Epidemiology of antituberculosis drug resistance (the Global Project on Anti-Tuberculosis Drug Resistance Surveillance): an updated analysis. Lancet 368:2142-2154.
6. Bastian, I., and F. Portaels. 2000. Multidrug-resistant tuberculosis: past, present and future, p. 1-15. In I. Bastian and F. Portaels (ed.), Multidrugresistant tuberculosis, 1st ed. Kluwer Academic Publishers, London, United Kingdom.
7. Canetti, G., W. Fox, A. Khomenko, H. T. Malher, N. K. Menon, D. A. Mitchison, N. Rist, and N. A. Smelev. 1969. Advances in techniques of testing mycobacterial drug sensitivity and the use of sensitivity tests in tuberculosis control programmes. Bull. W. H. O. 41:21-43.
8. Caviedes, L., T. S. Lee, R. H. Gilman, P. Sheen, E. Spellman, E. H. Lee, D. E. Berg, S. Montenegro-James, and the Tuberculosis Working Group in Peru. 2000. Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. J. Clin. Microbiol. 38:1203-1208.
9. Goloubeva, V., M. Lecocq, P. Lassowsky, F. Matthys, F. Portaels, and I. Bastian. 2001. Evaluation of mycobacteria growth indicator tube for direct and indirect drug susceptibility testing of Mycobacterium tuberculosis from respiratory specimens in a Siberian prison hospital. J. Clin. Microbiol. 39: 1501-1505.
10. Kent, P. T., and G. P. Kubica. 1985. Public health mycobacteriology. A guide for the level III laboratory. Centers for Disease Control and Prevention, Atlanta, GA.
11. Kiepiela, P., K. S. Bishop, A. N. Smith, L. Roux, and D. F. York. 2000. Genomic mutations in the katG, inhA and aphC genes are useful for the prediction of isoniazid resistance in Mycobacterium tuberculosis isolates from Kwazulu Natal, South Africa. Tuber. Lung Dis. 80:47-56.
12. Laserson, K. F., and C. D. Wells. 2007. Reaching the targets for tuberculosis control: the impact of HIV. Bull. W. H. O. 85:377-381.
13. Mathew, S., C. N. Paramasivan, F. Rehman, R. Balambal, K. Rajaram, and R. Prabhakar. 1995. A direct rifampicin sensitivity test for tubercle bacilli. Indian J. Med. Res. 102:99-103.
14. Musa, H. R., M. Ambroggi, A. Souto, and K. A. Angeby. 2005. Drug susceptibility testing of Mycobacterium tuberculosis by a nitrate reductase assay applied directly on microscopy-positive sputum samples. J. Clin. Microbiol. 43:3159-3161.
15. Palomino, J. C., A. Martin, M. Camacho, H. Guerra, J. Swings, and F. Portaels. 2002. Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 46:2720-2722.
16. Rigouts, L., O. Nolasco, P. de Rijk, E. Nduwamahoro, A. Van Deun, A. Ramsay, J. Arevalo, and F. Portaels. 2007. Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis. J. Clin. Microbiol. 45:252-254.
17. Roberts, G. D., N. L. Goodman, L. Heifets, H. W. Larsh, T. H. Lindner, J. K. McClatchy, M. R. McGinnis, S. H. Siddiqi, and P. Wright. 1983. Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. J. Clin. Microbiol. 18:689-696.
18. Solis, L. A., S. S. Shin, L. L. Han, F. Llanos, M. Stowell, and A. Sloutsky. 2005. Validation of a rapid method for detection of M. tuberculosis resistance to isoniazid and rifampin in Lima, Peru. Int. J. Tuber. Lung Dis. 9:760-764.
19. Syre, H., S. Phyu, P. Sandven, B. Bjorvatn, and H. M. Grewal. 2003. Rapid colorimetric method for testing susceptibility of Mycobacterium tuberculosis to isoniazid and rifampin in liquid cultures. J. Clin. Microbiol. 41:5173-5177.
20. Syre, H., H. Valvatne, P. Sandven, and H. M. Grewal. 2006. Evaluation of the nitrate-based colorimetric method for testing the susceptibility of Mycobacterium tuberculosis to streptomycin and ethambutol in liquid cultures. J. Antimicrob. Chemother. 57:987-991.
21. World Health Organization. 1998. Laboratory services in tuberculosis control. Part II. Microscopy. WHO, Geneva, Switzerland.
22. World Health Organization. 1998. Laboratory services in tuberculosis control. Part III. Culture. WHO, Geneva, Switzerland.
23. World Health Organization. 2004. The WHO/IUATLD global project on antituberculosis drug resistance surveillance. Antituberculosis drug resistance in the world, report no. 3. WHO/HTM/TB/2005.349. WHO, Geneva, Switzerland.
24. World Health Organization. 2007. Global tuberculosis control: surveillance, planning, financing. WHO report 2007. WHO/HTM/TB/2007.376. WHO, Geneva, Switzerland.