Validation of cultivation and PCR methods for diagnosis of Lyme neuroborreliosis

Journal of Clinical Microbiology

Volumn 46, Issue 10, 2008, Pages 3375-3379

  Cerar, Tjaša ^a,c   Ogrinc, Katarina ^b   Cimperman, Jože ^b   Lotrič Furlan, Stanka ^b   Strle, Franc ^b   Ružić Sabljić, Eva ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

^b UNIVERSITY MEDICAL CENTRE LJUBLJANA   (Slovenia)

^c UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; BLOOD SAMPLING; BORRELIA AFZELII; BORRELIA BURGDORFERI; CEREBROSPINAL FLUID ANALYSIS; CLINICAL ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; GENOTYPE; HUMAN; INTERMETHOD COMPARISON; LYME DISEASE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; TICK BORNE ENCEPHALITIS; VALIDATION STUDY;

ADULT; BACTERIOLOGICAL TECHNIQUES; BLOOD; BORRELIA BURGDORFERI GROUP; CEREBROSPINAL FLUID; DNA, BACTERIAL; HUMANS; LYME NEUROBORRELIOSIS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; PROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY;

BORRELIA AFZELII; BORRELIA BURGDORFERI GROUP; BORRELIA GARINII; IXODIDA;

EID: 53649085746     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00410-08     Document Type: Article

References (25)

1. Aguero-Rosenfeld, M. E., G. Wang, I. Schwartz, and G. P. Wormser. 2005. Diagnosis of Lyme borreliosis. Clin. Microbiol. Rev. 18:484-509.
2. Brouqui, P., F. Bacellar, G. Baranton, R. J. Birtles, A. Bjoërsdorff, J. R. Blanco, G. Caruso, M. Cinco, P. E. Fournier, E. Francavilla, M. Jensenius, J. Kazar, H. Laferl, A. Lakos, S. Lotric Furlan, M. Maurin, J. A. Oteo, P. Parola, C. Perez-Eid, O. Peter, D. Postic, D. Raoult, A. Tellez, Y. Tselentis, and B. Wilske. 2004. Guidelines for the diagnosis of tick-borne bacterial diseases in Europe. Clin. Microbiol. Infect. 10:1108-1132.
3. Busch, U., C. Hizo-Teufel, V. Fingerle, H. Nitschko, B. Wilske, and V. Preac-Mursic. 1996. Three species of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto, B. afzelii and B. garinii) identified from cerebrospinal fluid isolates by pulse-field gel electrophoresis and PCR. J. Clin. Microbiol. 34:1072-1078.
4. Cimperman, J., V. Maraspin, S. Lotrič-Furlan, E. Ružić-Sabljić, and F. Strle. 1999. Lyme meningitis: a one-year follow up controlled study. Wien. Klin. Wochenschr. 111:961-963.
5. Cimperman, J., V. Maraspin, S. Lotrič-Furlan, E. Ružić-Sabljić, T. Avšič-Županc, and F. Strle. 2002. Double infection with tick borne encephalitis virus and Borrelia burgdorferi sensu lato. Wien. Klin. Wochenschr. 114:620-622.
6. Coyle, P. K., and S. E. Schutzer. 2002. Neurologic aspects of Lyme disease. Med. Clin. N. Am. 86:261-284.
7. Demaerschalck, I., A. Ben Messaoud, M. De Kesel, B. Hoyois, Y. Lobet, P. Hoet, G. Bigaignon, A. Bollen, and E. Godfroid. 1995. Simultaneous presence of different Borrelia burgdorferi genospecies in biological fluids of Lyme disease patients. J. Clin. Microbiol. 33:602-608.
8. Greer, C. E., S. L. Peterson, N. B. Kiviat, and M. M. Manos. 1991. PCR amplification from paraffin-embedded tissues: effects of fixative and fixation time. Am. J. Clin. Pathol. 95:117-124.
9. Guy, E. C., and G. Stanek. 1991. Detection of Borrelia burgdorferi in patients with Lyme disease by polymerase chain reaction. J. Clin. Pathol. 44:610-611.
10. Karlsson, M., K. Hovind-Hougen, B. Svenungsson, and G. Stiernstedt. 1990. Cultivation and characterization of spirochetes from cerebrospinal fluid of patients with Lyme borreliosis. J. Clin. Microbiol. 28:473-479.
11. Korenberg, E. I., Y. V. Kovalevskii, A. S. Karanov, and G. G. Moskvitina. 1999. Mixed infections by tick-borne encephalitis virus and borrelia in ticks. Med. Vet. Entomol. 13:204-208.
12. Maraspin, V., E. Ružić-Sabljić, J. Cimperman, S. Lotrič-Furlan, T. Jurca, R. N. Picken, and F. Strle. 2001. Isolation of Borrelia burgdorferi sensu lato from blood of patients with erythema migrans. Infection 29:65-70.
13. Ornstein, K., J. Berglund, I. Nilsson, R. Norrby, and S. Bergström. 2001. Characterization of Lyme borreliosis isolates from patients with erythema migrans and neuroborreliosis in southern Sweden. J. Clin. Microbiol. 39:1294-1298.
14. Picken, R. N., Y. Cheng, F. Strle, J. Cimperman, V. Maraspin, S. Lotrič-Furlan, E. Ružić-Sabljić, D. Han, J. A. Nelson, M. M. Picken, and G. M. Trenholme. 1996. Molecular characterization of Borrelia burgdorferi sensu lato from Slovenia revealing significant differences between tick and human isolates. Eur. J. Clin. Microbiol. Infect. Dis. 15:313-323.
15. Postic, D., M. V. Assous, P. A. D. Grimont, and G. Baranton. 1994. Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int. J. Syst. Bacteriol. 44:743-752.
16. Ružić-Sabljić, E., V. Maraspin, S. Lotrić-Furlan, T. Jurca, M. Logar, A. Pikelj-Pečnik, and F. Strle. 2002. Characterization of Borrelia burgdorferi sensu lato strains isolated from human material in Slovenia. Wien. Klin. Wochenschr. 114:544-550.
17. Ružić-Sabljić, E., M. Arnež, M. Logar, V. Maraspin, S. Lotrič-Furlan, J. Cimperman, and F. Strle. 2005. Comparison of Borrelia burgdorferi sensu lato strains isolated from specimens obtained simultaneously from two different sites of infection in individual patients. J. Clin. Microbiol. 43:2194-2200.
18. Schmidt, B. L. 1997. PCR in laboratory diagnosis of human Borrelia burgdorferi infections. Clin. Microbiol. Rev. 10:185-201.
19. Stanek, G., and F. Strle. 2003. Lyme borreliosis. Lancet 362:1639-1647.
20. Steere, A. C. 2001. Lyme disease. N. Engl. J. Med. 345:115-125.
21. Strle, F., J. A. Nelson, E. Ružić-Sabljić, J. Cimperman, V. Maraspin, S. Lotric-Furlan, Y. Cheng, M. M. Picken, G. M. Trenholme, and R. N. Picken. 1996. European Lyme borreliosis: 231 culture-confirmed cases involving patients with erythema migrans. Clin. Infect. Dis. 23:61-65.
22. Strle, F. 1999. Lyme borreliosis in Slovenia. Zentralbl. Bakteriol. 289:643-652.
23. Strle, F., E. Ružić-Sabljić, J. Cimperman, S. Lotrič-Furlan, and V. Maraspin. 2006. Comparison of findings for patients with Borrelia garinii and Borrelia afzelii isolated from cerebrospinal fluid. Clin. Infect. Dis. 43:704-710.
24. Wilske, B., V. Fingerle, and U. Schulte-Spechtel. 2007. Microbiological diagnosis of Lyme borreliosis. FEMS Immunol. Med. Microbiol. 49:13-21.
25. Zore, A., E. Ružić-Sabljić, V. Maraspin, J. Cimperman, S. Lotrič-Furlan, A. Pikelj, T. Jurca, M. Logar, and F. Strle. 2002. Sensitivity of culture and PCR for the etiologic diagnosis of erythema migrans. Wien. Klin. Wochenschr. 114:606-609.