Carvacrol given to rats in drinking water reduces the level of DNA lesions induced in freshly isolated hepatocytes and testicular cells by H 2O2

Neoplasma

Volumn 55, Issue 5, 2008, Pages 394-399

  Slamenova, D ^a   Horvathova, E ^a   Marsalkova, L ^a   Wsolova, L ^b  

^a INSTITUTE OF CHEMISTRY   (Slovakia)

^b SLOVAK MEDICAL UNIVERSITY   (Slovakia)

Author keywords

Carvacrol; Hepatocytes; Single cell gel electrophoresis; Testicular cells

Indexed keywords

ANTIOXIDANT; CARVACROL; DRINKING WATER; HYDROGEN PEROXIDE; TERPENE;

ANIMAL EXPERIMENT; ANTIOXIDANT ACTIVITY; ARTICLE; CELL PROTECTION; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; GEL ELECTROPHORESIS; LIVER CELL; MALE; NONHUMAN; OXIDATIVE STRESS; RAT; TESTIS; TREATMENT DURATION; ANIMAL; COMET ASSAY; DRINKING; DRUG EFFECT; IN VITRO STUDY; SPRAGUE DAWLEY RAT;

ANIMALS; COMET ASSAY; DNA DAMAGE; DRINKING; HEPATOCYTES; HYDROGEN PEROXIDE; MALE; MONOTERPENES; RATS; RATS, SPRAGUE-DAWLEY; TESTIS;

EID: 53449084535     PISSN: 00282685     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. NAFISI S, HAJIAKHOONDI A, YEKTADOOST A. Thymol and carvacrol binding to DNA: model for drug-DNA interaction. Biopolymers 2004; 74: 345-351.
2. NAKATANI N. Antioxidative and antimicrobial constituents of herbs and spices. In: Spices, Herbs and Edible Fungi, Ed. G. Charalambous, Elsevier, New York 1994; pp. 251-271.
3. CHAMI N, CHAMI F, BENNIS S et al. Antifungal treatment with carvacrol and eugenol of oral candidiasis in immunosuppressed rats. Braz J Infect Dis 2004; 8: 217-226.
4. PINTO E, PINA-VAZ C, SALGUEIRO L et al. Antifungal activity of the essential oil of Thymus pulegioides on Candida, Aspergillus and dermatophyte species. J Med Microbiol 2006; 55: 1367-1373. (Pubitemid 44651663)
5. STAMMATI A, BONSI P, ZUCCO F et al. Toxicity of selected plant volatiles in microbial and mammalian short-term assays. Food Chem Toxicol 1999; 37: 813-823.
6. IPEK E, TÜYLÜ BA, ZEYTINOGLU H. Effects of carvacrol on sister chromatid exchanges in human lymphocyte cultures. Cytotechnology 2003; 43: 145-148.
7. AZIRAK S, RENCUZOGULLARI E. The in vivo genotoxic effects of carvacrol and thymol in rat bone marrow cells. Environ Toxicol 2008; 24: [Epub ahead of print]
8. HALLIWELL B, ARUOMA OI. DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems. FEBS Lett 1991; 281: 9-19.
9. EPE B, PFLAUM M, HÄRING M et al. Use of repair endonucleases to characterize DNA damage induced by reactive oxygen species in cellular and cell-free systems. Toxicol Lett 1993; 67: 57-72. (Pubitemid 23087361)
10. MICHALOPOULOS G, CIANCIULLI HD, NOVOTNY AR et al. Liver regeneration studies with rat hepatocytes in primary culture. Cancer Res 1982; 42: 4673-4682. (Pubitemid 13205972)
11. BRADLEY MO, DYSART G. DNA single-strand breaks, double-strand breaks, and crosslinks in rat testicular germ cells: measurements of their formation and repair by alkaline and neutral filter elution. Cell Biol Toxicol 1985; 1: 181-195.
12. SØDERLUND EJ, BRUNBORG G, OMICHINSKI JG et al. Testicular necrosis and DNA damage caused by denaturated and methylated analogs of 1,2-dibromo-3-chloropropane in the rat. Toxicol Appl Pharmacol 1988; 94: 437-447.
13. SINGH NP, McCOY MT, TICE RR et al. A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184-191.
14. SLAMEŇOVÁ D, GÁBELOVÁ A, RUŽ EKOVÁ L' et al. Detection of MNNG-induced DNA lesions in mammalian cells; validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations. Mutat Res 1997; 383: 243-252.
15. SLAMEŇOVÁ D, HORVÁTHOVÁ E, ŠRAMKOVÁ M et al. DNA-protective effects of two components of essential plant oils carvacrol and thymol on mammalian cells cultured in vitro. Neoplasma 2007; 54: 108-112.
16. MARKLUND SL, WESTMAN NG, LUNDGREN E et al. Copper- and zinc-containing superoxide dismutase, manganese-containing superoxide dismutase, catalase, and glutathione peroxidase in normal and neoplastic human cell lines and normal human tissues. Cancer Res 1982; 5: 1955-1961.
17. KALEMBA D, KUNICKA A. Antibacterial and antifungal properties of essential oils. Curr Med Chem 2003; 10: 813-829. (Pubitemid 36443237)
18. SLAMEŇOVÁ D, HORVÁTHOVÁ E, ŠRAMKOVÁ M et al. Toxic, genotoxic and DNA-protective effects of selected plant volatiles on human cells cultured in vitro. In: The activity of natural compounds in diseases prevention and therapy, Ed. Zdenka Ďuračková and Siegfried Knasmüller, Mind &Health Civil Asociation and the Slovak Academic Press LtD 2007, pp. 149-170.
19. AESCHBACH R, LOLIGER J, SCOTT BC et al. Antioxidant actions of thymol, carvacrol, 6-gingerol, zingerone and hydroxytyrosol. Food Chem Toxicol 1994; 32: 31-36.
20. SOKMEN M., SERKEDJIEVA J, DAFERERA D et al. In vitro antioxidant, antimicrobial, and antiviral activities of the essential oil and various extracts from herbal parts and callus cultures of Origanum acutidens. J Agric Food Chem 2004; 52: 3309-3312.
21. LIMA CF, ANDRADE PB, SEABRA RM et al. The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats. J Ethnopharmacol 2005; 97: 383-389.
22. HORVÁTHOVÁ E, ŠRAMKOVÁ M, LÁBAJ J et al. Study of cytotoxic, genotoxic and DNA-protective effects of selected plant essential oils on human cells cultured in vitro .Neuro Endocrinology Letters, Supplement 2, 2006; 44-47.
23. HORVÁTHOVÁ E, TURČÁNYOVÁ V, SLAMEŇOVÁ D. Comparative effects of DNA-damaging and DNA-protective effects of selected components of essential plant oils in human leukemic cells K562. Neoplasma 2007; 54: 478-483.