Short communication: Genetic transformation and shoot regeneration procedure for pepper (capsicum annuum L.)

Acta Agronomica Hungarica

Volumn 54, Issue 2, 2006, Pages 147-149

  Mihalka V ^a   Balazs E ^b  

^a SZENT ISTVÁN UNIVERSITY   (Hungary)

^b INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

Methodology; Pepper regeneration; Recalcitrant species; Stable transformation

Indexed keywords

EID: 53249149862     PISSN: 02380161     EISSN: 15882527     Source Type: Journal    
DOI: 10.1556/AAgr.54.2006.2.4     Document Type: Article

References (5)

1. Chilton, M. D., Currier, T. C., Farrand, S. K., Bendich, A. J., Gordon, M. P., Nester, E. W. (1974): Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc. Nat. Acad. Sci. USA, 71, 3672-3676.
2. Ditta, G., Stanfield, S., Cobbin, D., Helinski, D. R. (1980): Broad host range DNA cloning system for Gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc. Nat. Acad. Sci. USA, 77, 7347-7351.
3. Figurski, D. H., Helinski, D. R. (1979): Replication of an origin-containing derivative of plasmid function provided in trans. Proc. Nat. Acad. Sci. USA, 76, 1648-1652.
4. Mihálka, V., Fári, M., Szász, A., Balázs, E., Nagy, I. (2003): Optimized protocols for efficient plant regeneration and gene transfer in pepper (Capsicum annuum L.). J. Plant Biotechnol., 2, 143-149.
5. Mihálka, V., Balázs, E., Nagy, I. (2003): Binary transformation system based on shooter mutants of Agrobacterium tumefaciens: A simple, efficient and universal gene transfer technology allowing marker gene elimination. Plant Cell Reports, 21, 778-784.