Polyethylene glycol conjugation enhances the nitrite reductase activity of native and cross-linked hemoglobin

Biochemistry

Volumn 47, Issue 40, 2008, Pages 10773-10780

  Lui, Francine E ^a   Dong, Pengcheng ^a   Kluger, Ronald ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ETHYLENE GLYCOL; GLYCOLS; NITRIC OXIDE; OXIDES; OXYGEN; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; RATE CONSTANTS; THERMOPLASTICS;

CELL-FREE; IN-VIVO; MULTIPLE CHAINS; NITRITE IONS; NITRITE REDUCTASE; RED CELLS; VASOACTIVITY;

HEMOGLOBIN;

FUMARYLACETOACETASE; HEME; HEMOGLOBIN; MACROGOL; NITRIC OXIDE; NITRITE; NITRITE REDUCTASE;

ARTICLE; BLOOD VESSEL REACTIVITY; DRUG CONJUGATION; ENZYME ACTIVATION; ERYTHROCYTE TRANSFUSION; HEMOGLOBIN DETERMINATION; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; HUMAN TISSUE; IN VIVO STUDY; MOLECULAR STABILITY; NORMAL HUMAN; OXYGEN TRANSPORT; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN STABILITY; REACTION ANALYSIS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; DITHIONITROBENZOIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEMOGLOBINS; HUMANS; KINETICS; LINEAR MODELS; MOLECULAR STRUCTURE; NITRIC OXIDE; NITRITE REDUCTASES; POLYETHYLENE GLYCOLS;

EID: 53249134617     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi801116k     Document Type: Article

References (25)

1. Winslow, R. M. (2003) Current status of blood substitute research: Towards a new paradigm. J. Intern. Med. 253, 508-517.
2. Jahr, J. S., Walker, V., and Manoochehri, K. (2007) Blood substitutes as pharmacotherapies in clinical practice. Curr. Opin. Anaesthesiol. 20, 325-330.
3. Tsai, A. G., Cabrales, P., Manjula, B. N., Acharya, S. A., Winslow, R. M., and Intaglietta, M. (2006) Dissociation of local nitric oxide concentration and vasoconstriction in the presence of cell-free hemoglobin oxygen carriers. Blood 108, 3603-3610.
4. 2 binding to a maleimide poly(ethylene glycol)-conjugated human haemoglobin. Biochem. J. 382, 183-189.
5. Rohlfs, R. J., Bruner, E., Chiu, A., Gonzales, A., Gonzales, M. L., Magde, D., Magde, M. D., Jr., Vandegriff, K. D., and Winslow, R. M. (1998) Arterial blood pressure responses to cell-free hemoglobin solutions and the reaction with nitric oxide. J. Biol. Chem. 273, 12128-12134.
6. Gladwin, M. T., Raat, N. J., Shiva, S., Dezfulian, C., Hogg, N., Kim-Shapiro, D. B., and Patel, R. P. (2006) Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilation. Am. J. Physiol. 291, H2026-H2035.
7. Huang, K. T., Keszler, A., Patel, N., Patel, R. P., Gladwin, M. T., Kim-Shapiro, D. B., and Hogg, N. (2005) The reaction between nitrite and deoxyhemoglobin. Reassessment of reaction kinetics and stoichiometry. J. Biol. Chem. 280, 31126-31131.
8. Huang, Z., Shiva, S., Kim-Shapiro, D. B., Patel, R. P., Ringwood, L. A., Irby, C. E., Huang, K. T., Ho, C., Hogg, N., Schechter, A. N., and Gladwin, M. T. (2005) Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control. J. Clin. Invest. 115, 2099-2107.
9. Doyle, M. P., Pickering, R. A., DeWeert, T. M., Hoekstra, J. W., and Pater, D. (1981) Kinetics and mechanism of the oxidation of human deoxyhemoglobin by nitrites. J. Biol. Chem. 256, 12393-12398.
10. Jones, R. T. (1994) Structural characterization of modified hemoglobins. Methods Enzymol. 231, 322-343.
11. Walder, R. Y., Andracki, M. E., and Walder, J. A. (1994) Preparation of intramolecularly cross-linked hemoglobins. Methods Enzymol. 231, 274-280.
12. Snyder, S. R., Welty, E. V., Walder, R. Y., Williams, L. A., and Walder, J. A. (1987) HbXL99 α: A hemoglobin derivative that is cross-linked between the α subunits is useful as a blood substitute. Proc. Natl. Acad. Sci. U.S.A. 84, 7280-7284.
13. Vandegriff, K. D., Malavalli, A., Wooldridge, J., Lohman, J., and Winslow, R. M. (2003) MP4, a new nonvasoactive PEG-Hb conjugate. Transfusion 43, 509-516.
14. Jones, R. T., Shih, D. T., Fujita, T. S., Song, Y., Xiao, H., Head, C., and Kluger, R. (1996) A doubly cross-linked human hemoglobin. Effects of cross-links between different subunits. J. Biol. Chem. 271, 675-680.
15. Wright, S. K., and Viola, R. E. (1998) Evaluation of methods for the quantitation of cysteines in proteins. Anal. Biochem. 265, 8-14.
16. Manjula, B. N., Tsai, A. G., Intaglietta, M., Tsai, C. H., Ho, C., Smith, P. K., Perumalsamy, K., Kanika, N. D., Friedman, J. M., and Acharya, S. A. (2005) Conjugation of multiple copies of polyethylene glycol to hemoglobin facilitated through thiolation: Influence on hemoglobin structure and function. Protein J. 24, 133-146.
17. 6-HbA is non-hypertensive. Artif Cells, Blood Substitutes, Immobilization Biotechnol. 33, 239-255.
18. Veronese, F. M., and Harris, J. M. (2002) Introduction and overview of peptide and protein pegylation. Adv. Drug Delivery Rev. 54, 453-456.
19. Shiva, S., Huang, Z., Grubina, R., Sun, J., Ringwood, L. A., MacArthur, P. H., Xu, X., Murphy, E., Darley-Usmar, V. M., and Gladwin, M. T. (2007) Deoxymyoglobin is a nitrite reductase that generates nitric oxide and regulates mitochondrial respiration. Circ. Res. 100, 654-661.
20. Cosby, K., Partovi, K. S., Crawford, J. H., Patel, R. P., Reiter, C. D., Martyr, S., Yang, B. K., Waclawiw, M. A., Zalos, G., Xu, X., Huang, K. T., Shields, H., Kim-Shapiro, D. B., Schechter, A. N., Cannon, R. O., III, and Gladwin, M. T. (2003) Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation. Nat. Med. 9, 1498-1505.
21. 2 during extreme hemodilution with hemoglobin site specifically PEGylated at Cys93(β) in hamster window chamber. Am. J. Physiol. 287, H1609-H1617.
22. Harris, J. M., and Chess, R. B. (2003) Effect of pegylation on pharmaceuticals. Nat. Rev. Drug Discovery 2, 214-221.
23. Dai, S., and Tam, K. C. (2006) Effect of cosolvents on the binding interaction between poly(ethylene oxide) and sodium dodecyl sulfate. J. Phys. Chem. B 110, 20794-20800.
24. Morar, A. S., Schrimsher, J. R. L., and Chavez, M. D. (2006) PEGylation of proteins: A structural approach. Biopharm. Int. 19, 34-47.
25. 3 by the concerted nitrite reductase and anhydrase activity of hemoglobin. Nat. Chem. Biol. 3, 785-794.