High-sensitivity peptide mapping by micro-LC with on-line membrane blotting and subsequent detection by scanning-IR-MALDI mass spectrometry

Journal of Protein Chemistry

Volumn 16, Issue 5, 1997, Pages 349-362

  Eckerskorn, Christoph ^a,b   Strupat, Kerstin ^a,b   Kellermann, Josef ^a   Lottspeich, Friedrich ^a   Hillenkamp, Franz ^b  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

ESI MS; Micro LC; Peptide mapping; Scanning IR MALDI MS; Sequencing

Indexed keywords

AMINO ACID SEQUENCE; CONFERENCE PAPER; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; PEPTIDE MAPPING;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; MASS SPECTROMETRY; MEMBRANES, ARTIFICIAL; MICROCHEMISTRY; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; POLYVINYLS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPECTROPHOTOMETRY, INFRARED; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 53249129192     PISSN: 02778033     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026324419398     Document Type: Conference Paper

References (12)

1. Biais, J. C., Nagnan-Le-Meillour, P., Bolbach, G., and Tabet, J. C. (1996). Rapid Commun. Mass Spectrom. 10, 1-4.
2. Covey, T. R., Bronner, R. F., Shushan, B. I., and Henion, J. (1988). Rapid Commun. Mass Spectrom. 2, 249-256.
3. Eckerskorn, C., and Grimm, R. (1996). Electrophoresis 17, 899-906.
4. Eckerskorn, C., Strupat, K., Karas, M., Hillenkamp, F., and Lottspeich, F. (1992). Electrophoresis 13, 664-665.
5. Hsi, K.-L., Kochersperger, M. L., Werner, W. E., Grimley, C., Ly, H., Sandell, S., and Yuan, P. M. (1995). In PE Applied Biosystems: "Moving Forward in Protein Analysis" 1995, Posterior Presentations, pp. 1-10.
6. Mehl, E., Plambeck, J., and Lottspeich, F. (1993). In Würzburger Kolloquium 1993 (Engelhardt, H., ed.), Bertsch, Straubing, pp. 189-195.
7. Schreiner, M., Strupat, K., Lottspeich, F., and Eckerskorn, C. (1996). Electrophoresis 17, 954-961.
8. Strupat, K., Eckerskorn, C., Karas, M., and Hillenkamp, F. (1994a). In Proceedings of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics.
9. Strupat, K., Karas, M., Hillenkamp, F., Eckerskorn, C., and Lottspeich, F. (1994b). Anal. Chem. 66, 464-470.
10. Vestling, M., and Fenselau, C. (1994). Anal. Chem. 64, 471-476.
11. Zhang, H., and Caprioli, R. M. (1996a). In Proceedings of the 44nd ASMS Conference on Mass Spectrometry and Allied Topics, THP237.
12. Zhang, H., and Caprioli, R. M. (1996b). J. Mass Spectrom. 31, 690-692.