Comparison of white spot syndrome virus PCR-detection methods that use electrophoresis or antibody-mediated lateral flow chromatographic strips to visualize PCR amplicons

Journal of Virological Methods

Volumn 153, Issue 2, 2008, Pages 129-133

  Srisala, Jiraporn ^a,b   Tacon, Philip ^a   Flegel, Timothy W ^b   Sritunyalucksana, Kallaya ^a,b  

^a NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

^b MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Ethidium bromide; Gel electrophoresis alternative; Lateral flow chromatography detection strip (LFCS); White spot syndrome virus (WSSV)

Indexed keywords

ANTIBODY;

ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; ELECTROPHORESIS; GEL ELECTROPHORESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VIRUS DETECTION; WHITE SPOT SYNDROME VIRUS;

ANIMALS; ANTIBODIES, VIRAL; CHROMATOGRAPHY; ELECTROPHORESIS, AGAR GEL; PENAEIDAE; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WHITE SPOT SYNDROME VIRUS 1;

DECAPODA (CRUSTACEA); SHRIMP WHITE SPOT SYNDROME VIRUS;

EID: 53249107533     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.07.027     Document Type: Article

References (7)

1. Flegel T.W. Detection of major penaeid shrimp viruses in Asia, a historical perspective with emphasis on Thailand. Aquaculture 258 (2006) 1-33
2. Hsu H.C., Lo C.F., Lin S.C., Liu K.F., Peng S.E., Chang Y.S., Chen L.L., Liu J., and Kou G.H. Studies on effective PCR screening strategies for white spot syndrome virus (WSSV) detection in Penaeus monodon brooders. Dis. Aquat. Org. 39 (1999) 13-19
3. Lightner D.V., Hasson K.W., White B.L., and Redman R.M. Experimental infection of western hemisphere penaeid shrimp with Asian white spot syndrome virus and Asian yellow head virus. Dis. Aquat. Org. 10 (1998) 271-281
4. Lo C.F., Chang Y.S., Cheng C.T., and Kou G.H. PCR monitoring of cultured shrimp for white spot syndrome virus (WSSV) infection in growout ponds. In: Flegel T.W. (Ed). Advances in Shrimp Biotechnology (1998), National Center for Genetic Engineering and Biotechnology, Bangkok 281-286
5. ®, a immunochromatographic assay for white spot syndrome virus (WSSV), using quantitative real-time PCR. Aquaculture 257 (2006) 167-172
6. Sithigorngul W., Rukpratanporn S., Pecharaburanin N., Longyant S., Chaivisuthangkura P., and Sithigorngul P. A simple and rapid immunochromatographic strip for detection of white spot syndrome virus (WSSV) of shrimp. Dis. Aquat. Org. 72 (2006) 101-106
7. Sritunyalucksana K., Srisala J., McColl K., Nielsen L., and Flegel T.W. Comparison of PCR testing methods for white spot syndrome virus (WSSV) infections in penaeid shrimp. Aquaculture 255 (2006) 95-104