Synthesis and biochemical characterization of obligatory dimers of the sugar non-specific nuclease from Serratia marcescens using specifically designed bismaleimidoalkanes as SH-specific crosslinking reagents

Journal of Protein Chemistry

Volumn 18, Issue 1, 1999, Pages 137-146

  Franke, Ingo ^a   Pingoud, Alfred ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

Circular dichroism; Crosslink; Monomer dimer equilibrium; Peptide mapping; Site directed mutagenesis

Indexed keywords

CYSTEINE; MALEIMIDE DERIVATIVE; NUCLEASE; THIOL GROUP;

ALPHA HELIX; ARTICLE; CIRCULAR DICHROISM; COVALENT BOND; CROSS LINKING; ENZYME ANALYSIS; ENZYME SUBUNIT; ENZYME SYNTHESIS; GENETIC ENGINEERING; NONHUMAN; PEPTIDE MAPPING; PROTEIN SECONDARY STRUCTURE; SERRATIA MARCESCENS; SITE DIRECTED MUTAGENESIS;

SERRATIA; SERRATIA MARCESCENS;

EID: 53149140261     PISSN: 02778033     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1020616020507     Document Type: Article

References (28)

1. Ball, T. K., Saurugger, P. N. and Benedik, M. J. (1987) The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli, Gene 57, 183-92.
2. Ball, T. K., Sih, Y., and Benedik, M. J. (1992) Disulfide bonds are required for Serratia marcescens nuclease activity, Nucleic Acids Res. 20, 4971-4974.
3. Chen, L. Y., Ho, H. C., Tsai, Y. C. and Liao, T. H. (1993) Deoxyribonuclease of Syncephalastrum racemosum: enzymatic properties and molecular structure, Arch Biochem Biophys 303, 51-56.
4. Cheronis, J. C., Whalley, E. T., Nguyen, K. T., Eubanks, S. R., Allen, L. G., Duggan, M. J., Loy, S. D., Bonham, K. A., and Blodgett, J. K. (1992) J. Med. Chem. 35, 1563-1572.
5. Dake, E., Hofmann, T. J., McIntire, S., Hudson, A. and Zassenhaus, H. P. (1988) Purification and properties of the major nuclease from mitochondria of Saccharomyces cerevisiae, J. Biol. Chem. 263, 7691-7702.
6. Eaves, G. N. and Jeffries, C. D.: (1963) Isolation and properties of an exocellular nuclease of Serratia marcescens, J. Bacteriol. 85, 273-278.
7. Friedhoff, P., Gimadutdinow, O., Rüter, T., Wende, W., Urbanke, C., Thole, H., and Pingoud, A. (1994a) A procedure for renaturation and purification of the extracellular Serratia marcescens nuclease from genetically engineered Escherichia coli, Prot. Express. Purif. 5, 37-43.
8. Friedhoff, P., Gimadutinow, O., and Pingoud, A. (1994b) Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis, Nucleic Acids Res. 22, 3280-3287.
9. Friedhoff, P., Kolmes, B., Gimadutdinow, O., Wende, W., Krause, K. L. and Pingoud, A. (1996a) Analysis of the mechanism of the Serratia nuclease using site directed mutagenesis, Nucleic Acids Res. 24(14), 2632-2639.
10. Friedhoff, P., Meiss, G., Kolmes, B., Pieper, U., Gimadutdinow, O., Urbanke, C. and Pingoud, A. (1996b) Kinetic analysis of the cleavage of natural and synthetic substrates by the Serratia nuclease, Eur. J. Biochem. 241, 572-580.
11. Ikeda, S., Maeda, N., Ohshima, T. and Takata, N. (1996) Identification and characterization of a mitochondrial endonuclease from yeast, Schizosaccharomyces pombe, Biochem. Mol. Biol. Intern. 40, 1017-1024.
12. Kolmes, B., Franke, I., Friedhoff, P. and Pingoud, A. (1996) Analysis of the reaction mechanism of the non-specific endonuclease of Serratia marcescens using an artificial substrate, FEBS Lett. 397, 343-346.
13. Kunitz, M. (1950) Crystalline deoxyribonuclease I. Isolation and general properties. Spectrophotometric method for the measurement of deoxyribonuclease activity, J. Gen. Physiol. 33, 349-362
14. Lacks, S. & Neuberger, M. (1975) Membrane location of a deoxyri-bonuclease implicated in the genetic transformation of Diplococcus pneumoniae, J. Bacteriol. 124, 1321-1329.
15. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of bacteriophage T4, Nature 227, 680.
16. Meiss, G., Franke, I., Gimadutdinow, O., Urbanke, C. and Pingoud, A. (1998) Biochemical characterization of Anabaena sp. strain PCC 7120 non-specific nuclease NucA and ist inhibitor NuiA, Eur. J. Biochem. in press.
17. Meiss, G., Friedhoff, P., Hahn, M., Gimadutdinow, O., and Pingoud, A. (1995) Sequence preferences in cleavage of dsDNA and ssDNA by the extracellular Serratia marcescens endonuclease, Biochemistry 34, 11979-11988.
18. Metha, N. B., Phillips, A. P., Lui, F. F., and Brooks, R. E. (1960) Maleamic and Citraconamic Acids, Methyl Esters, and Imides, J. Org. Chem. 25, 1012-1015.
19. Miller, M. D., and Krause, K. L. (1996) Identification of the Serratia endonuclease dimer: Structural basis and implications for catalysis, Protein Science 5, 24-33
20. Miller, M. D., Tanner, J., Alpaugh, M., Benedik, M. J., and Krause, K. L. (1994) 2.1 Å structure of Serratia endonuclease suggests a mechanism for binding to double-stranded DNA, Structural Biology 1, 461-468.
21. Muro-Pastor, A. M., Flores, E., Herrero, A. and Wolk, C. P. (1992) Identification, genetic analysis and characterization of a sugar-non-specific nuclease from the cyanobacterium Anabaena sp. strain PCC 7120, Mol. Microbiol. 6, 3021-3030.
22. Muro-Pastor, A. M., Kuritz, T., Flores, E., Herrero, A. and Wolk, C. P. (1994). Transfer of a genetic marker from a megaplasmid of Anabaena sp. strain PCC 7120 to a megaplasmid of a different Anabaena strain, J. Bacteriology 176, 1093-1098.
23. Nestle, M. and Roberts, W. K. (1969a). An extracellular nuclease from Serratia marcescens - I. Purification and some properties of the enzyme, J. Biol. Chem. 244, 5213-5218.
24. Puyet, A., Greenberg, B. and Lacks, S. A. (1990) Genetic and structural characterization of EndA: A membrane-bound nuclease required for transformation of Streptococcus pneumoniae, J. Mol. Biol. 213, 727-738.
25. Rosenthal, A. L. and Lacks, S. A. (1977) Nuclease detection in SDS-polyacrylamide gel electrophoresis, Anal. Biochem. 80, 76-90.
26. Ruiz-Carrillo, A. and Cote, J. (1993) Primers for mitochondrial DNA replication generated by endonuclease G, Science 261, 765-769.
27. Vincent, R. D., Hofmann, T. J. and Zassenhaus, H. P. (1988) Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisae, Nucleic Acids Res. 16, 3297-3312.
28. Yonemura, K., Matsumoto, K. and Maeda, H. (1983) Isolation and characterization of nucleases from a clinical isolate of Serratia marcescens kums 3958, J. Biochem. 93, 1287-1295.