Probing the critical residues for intramolecular fructosyl transfer reaction of a levan fructotransferase

Journal of Microbiology and Biotechnology

Volumn 18, Issue 6, 2008, Pages 1064-1069

  Moon, Keum Ok ^a   Choi, Kyoung Hwa ^a   Kang, Ho Young ^a   Oh, Jeong Il ^a   Jang, Se Bok ^a   Park, Cheon Seok ^b   Lee, Jong Hoon ^c   Cha, Jaeho ^a  

^a Pusan National University   (South Korea)

^b Kyung Hee University   (South Korea)

^c Kyonggi University   (South Korea)

Author keywords

DFAIV; Error prone PCR mutagenesis; Levan fructotransferase; Microbacterium sp.; Transfructosylation

Indexed keywords

LEVAN; LEVAN FRUCTOTRANSFERASE; LEVANASE; LEVANBIOSE; TRANSFERASE; UNCLASSIFIED DRUG; DIFRUCTOSE ANHYDRIDE IV; DISACCHARIDE; FRUCTAN; GLYCOSYLTRANSFERASE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME MODIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; MUTANT; NONHUMAN; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; TRANSFRUCTOSYLATION; WILD TYPE; ACTINOMYCETALES; BINDING SITE; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MUTAGENESIS; THIN LAYER CHROMATOGRAPHY;

MICROBACTERIUM SP.; MICROBACTERIUM SP. AL-210;

ACTINOMYCETALES; BINDING SITES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, THIN LAYER; DISACCHARIDES; ESCHERICHIA COLI; FRUCTANS; HEXOSYLTRANSFERASES; HYDROLYSIS; MUTAGENESIS; POLYMERASE CHAIN REACTION;

EID: 53149084946     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Alberto, F., E. Jordi, B. Henrissat, and M. Czjzek. 2004. Crystal structure of inactivated Thermotoga maritima invertase in complex with the trisaccharide substrate raffinose. Biochem. J. 395: 457-462.
2. Avigad, G. and S. Bauer. 1996. Fructan hydrolases. Methods Enzymol. 8: 621-628.
3. Avigad, G. and R. Zelikson. 1963. Cleavage of fructans to levanbiose by a specific hydrolase. Bull. Res. Counc. Isr. 11A4: 253-257.
4. Cairns, A. J. 2003. Fructan biosynthesis in transgenic plants. J. Exp. Bot. 54: 549-567.
5. Cha, J., N. H. Park, S. J. Yang, and T. H. Lee. 2001. Molecular and enzymatic characterization of a levan fructotransferase from Microbacterium sp. AL-210. J. Biotechnol. 91: 49-61.
6. Han, Y. W. 1990. Microbial levan. Adv. Appl. Microbiol. 35: 171-194.
7. Hendry, G. 1987. The ecological significance of fructan in a contemporary flora. New Phytol. 106: 201-216.
8. Jung, Y.-J., C. S. Park, H. G. Lee, and J. Cha. 2006. Isolation of a novel gellan-depolymerizing Bacillus sp. strain YJ-1. J. Microbiol. Biotechnol. 16: 1868-1873.
9. Kim, M. J., H. E. Park, H. K. Sung, T. H. Park, and J. Cha. 2005. Action mechanism of transfructosylation catalyzed by Microbacterium laevaniformans levansucrase. J. Microbiol. Biotechnol. 15: 99-104.
10. Lee, C. Y., K. H. Kim, S. Y. Hur, J.-H. Heo, M. H. Choi, S. K. Rhee, and C. H. Kim. 2006. Enzymatic synthesis of ascorbic acid fructoside by transfructosylation using levan fructotransferase. J. Microbiol. Biotechnol. 16: 64-67.
11. Leemhuis, H., G. J. Euverink, and L. Dijkhuizen. 2003. High-throughput screening for gene libraries expressing carbohydrate hydrolase activity. Biotechnol. Lett. 25: 1643-1645.
12. Meng, G. and K. Fütterer. 2003. Structural framework of fructosyl transfer in Bacillus subtilis levansucrase. Nat. Struct. Biol. 10: 935-941.
13. Nagem, R. A. P., A. L. Rojas, A. M. Golubev, O. S. Korneeva, E. V. Eneyskaya, A. A. Kulminskaya, K. N. Neustrov, and I. Polikarpov. 2004. Crystal structure of exo-inulinase from Aspergillus awamori: The enzyme fold and structural determinants of substrate recognition. J. Mol. Biol. 344: 471-480.
14. Saito, K., H. Goto, A. Yokota, and F. Tomita. 1997. Purification of levan fructotransferase from Arthrobacter nicotinovorans GS-9 and production of DFAIV from levan by the enzyme. Biosci. Biotech. Biochem. 61: 1705-1709.
15. Song, E. K., H. Kim, H. K. Sung, and J. Cha. 2002. Cloning and characterization of a levanbiohydrolase from Microbacterium laevaniformans ATCC 15953. Gene 291: 45-55.
16. Tanaka, K., T. Uchiyama, K. Yamauchi, U. Suzuki, and S. Hashiguchi. 1982. Formation of a di-D-fructose dianhydride from levan by Arthrobacter ureafaciens. Carbohydr. Res. 99: 197-204.
17. Tanaka, K., T. Karigane, F. Yamaguchi, S. Nishikawa, and N. Yoshida. 1983. Action of levan frutosyltransferase of Arthrobacter ureafaciens on levanoligosaccharide. J. Biochem. 94: 1569-1578.
18. Tanaka, K., M. Shimonishi, M. Kitagaki, and M. Ikunaga. 1990. Action of levan fructotransferase of Arthrobacter ureafaciens on three oligosaccharides containing a bifurcose residue. Agric. Biol. Chem. 54: 815-817.
19. Verhaest, M., W. V. Ende, K. L. Roy, C. J. De Ranter, A. V. Laere, and A. Rabijns. 2005. X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: Fructan 1-exohydrolase IIa of Cichorium intybus. Plant J. 41: 400-411.
20. Yang, S. J., N. H. Park, T. H. Lee, and J. Cha. 2002. Expression, purification and characterization of a recombinant levan fructotransferase. Biotechnol. Appl. Biochem. 35: 199-203.
21. Yokota, A., K. Kondo, M. Nakagawa, I. Kojima, and F. Tomita. 1993. Production of levanbiose by a lvan-degrading enzyme from Streptomyces exfoliatus. Biosci. Biotech. Biochem. 57: 745-749.