Flippase activity in proteoliposomes reconstituted with Spinacea oleracea endoplasmic reticulum membrane proteins: Evidence of biogenic membrane flippase in plants

Biochemistry

Volumn 47, Issue 39, 2008, Pages 10481-10490

  Sahu, Santosh Kumar ^a   Gummadi, Sathyanarayana N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY MADRAS   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CELL MEMBRANES; FLIP FLOP CIRCUITS; LIFE SUPPORT SYSTEMS (SPACECRAFT); PHOSPHOLIPIDS; PROTEINS;

CYTOPLASMIC MEMBRANES; ENDOPLASMIC RETICULUM; ENDOPLASMIC RETICULUM MEMBRANES; EUKARYOTIC CELLS; FLIPPASE; IN PLANTS; MEMBRANE BIOGENESIS; METABOLIC ENERGY; MODEL SYSTEMS; PHOSPHATIDYL CHOLINE; PHOSPHATIDYL ETHANOLAMINE; PROTEOLIPOSOMES; RAT LIVERS; TRANSLOCATOR; TRITON X-100;

MEMBRANES;

ADENOSINE TRIPHOSPHATE; CALCIUM; CARRIER PROTEIN; FLIPPASE; FLUORESCENT DYE; MEMBRANE ENZYME; MEMBRANE PROTEIN; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHOLIPID; PROTEINASE; REAGENT; SCRAMBLASE; TRITON X 100; UNCLASSIFIED DRUG;

ARTICLE; BIOGENESIS; CELL MEMBRANE; ENDOPLASMIC RETICULUM; ENERGY; ENZYME ACTIVITY; ENZYME ASSAY; EUKARYOTIC CELL; MEMBRANE VESICLE; NONHUMAN; PRIORITY JOURNAL; PROTEOLIPOSOME; SPINACH;

CASP8 AND FADD-LIKE APOPTOSIS REGULATING PROTEIN; CELL MEMBRANE; ENDOPLASMIC RETICULUM; LIPOSOMES; MEMBRANE PROTEINS; PHOSPHOLIPIDS; PLANT LEAVES; PLANT PROTEINS; PROTEOLIPIDS; SPINACIA OLERACEA;

ANIMALIA; BACTERIA (MICROORGANISMS); EUKARYOTA; RATTUS; SPINACIA OLERACEA;

EID: 52949121441     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi8014339     Document Type: Article

References (33)

1. Menon, A. K. (1995) Flippases. Trends Cell Biol. 5, 355-360.
2. Gummadi, S. N., and Kumar, K. S. (2004) Mystery of phospholipid flip-flop in biogenic membranes. Cell. Mol. Biol. Lett. 9, 101-121.
3. Bell, R. M., Ballas, L. M., and Coleman, R. A. (1981) Lipid topogenesis. J. Lipid Res. 22, 391-403.
4. Moreau, P., Bessoule, J. J., Mongrand, S., Testest, T., Vincent, P., and Cassagne, C. (1998) Lipid trafficking in plant cells. Prog. Lipid Res. 37, 371-391.
5. Roughan, P. G., and Slack, C. R. (1984) Glycerolipid synthesis in leaves. Trends Biochem. Sci. 9, 383-386.
6. Kagawa, T., Lord, J. M., and Beevers, H. (1973) The origin and turnover of organelle membranes in castor bean endosperm. Plant Physiol. 51, 61-65.
7. Vandor, S. L., and Richardson, K. E. (1968) Incorporation of ethanolamine-1,2-14 C into plant microsomal phospholipids. Can. J. Biochem. 46, 1309-1315.
8. Andrews, J., and Keegstra, K. (1983) Acyl-CoA synthetase is located in the outer membrane and acyl-CoA thioesterase in the inner membrane of pea chloroplast envelopes. Plant Physiol. 72, 735-740.
9. Rothman, J. E., and Kennedy, E. P. (1977) Rapid transmembrane movement of newly synthesized phospholipids during membrane assembly. Proc. Natl. Acad. Sci. U.S.A. 74, 1821-1825.
10. Bishop, W. R., and Bell, R. M. (1985) Assembly of the endoplasmic reticulum phospholipid bilayer: The phosphatidylcholine transporter. Cell 42, 51-60.
11. Buton, X., Morrot, G., Fellmann, P., and Seigneuret, M. (1996) Ultrafast glycerophospholipid-selective transbilayer motion mediated by a protein in the endoplasmic reticulum membrane. J. Biol. Chem. 271, 6651-6657.
12. Kubelt, J., Menon, A. K., Müller, P., and Herrmann, A. (2002) Transbilayer movement of fluorescent phospholipid analogues in the cytoplasmic membrane of Escherichia coli. Biochemistry 41, 5605-5612.
13. Hrafnsdo'ttir, S., Nicholas, J. W., and Menon, A. K. (1997) Transbilayer movement of fluorescent phospholipids in Bacillus megaterium membrane vesicles. Biochemistry 36, 4969-4978.
14. Hrafnsdo'ttir, S., and Menon, A. K. (2000) Reconstitution and partial characterization of phospholipid flippase activity from detergent extracts of Bacillus subtilis cell membrane. J. Bacteriol. 182, 4198-206.
15. Gummadi, S. N., and Menon, A. K. (2002) Transbilayer movement of dipalmitoylphosphatidylcholine in proteoliposomes reconstituted from detergent extracts of endoplasmic reticulum: Kinetics of transbilayer transport mediated by a single flippase and identification of protein fractions enriched in flippase activity. J. Biol. Chem. 277, 25337-25343.
16. Chang, Q., Gummadi, S. N., and Menon, A. K. (2004) Chemical modification identifies two populations of glycerophospholipid flippase in rat liver ER. Biochemistry 43, 10710-10718.
17. Menon, A. K., Watkins, W. E., and Hrafnsdo'ttir, S. (2000) Specific proteins are required to translocate phosphatidylcholine bidirectionally across the endoplasmic reticulum. Curr. Biol. 10, 241-252.
18. Backer, J. M., and Dawidowicz, E. A. (1987) Reconstitution of a phospholipid flippase from rat liver microsomes. Nature 327, 341-343.
19. Sanyal, S., Frank, C. G., and Menon, A. K. (2008) Distinct flippases translocate glycerophospholipids and oligosachharide diphosphate across the endoplasmic reticulum. Biochemistry 47, 7937-7946.
20. Sahu, S. K., Gummadi, S. N., Manoj, N., and Aradhyam, G. K. (2007) Phospholipid scramblases: An overview. Arch. Biochem. Biophys. 462, 103-114.
21. 2+ dependent transbilayer movement of phospholipid. J. Biol. Chem. 271, 17205-17210.
22. Lord, J. M., Kiagawva, T., and Beevers, H. (1972) Intracellular distribution of enzymes of the cytidine diphosphate choline pathway in castor bean endosperm. Proc. Natl. Acad. Sci. U.S.A. 69, 2429-2432.
23. Buege, J. A., and Aust, S. D. (1975) Comparative studies of rat liver and lung NADPH-cytochrome c reductase. Biochim. Biophys. Acta 385, 371-379.
24. Lèvy, D., Bluzat, A., Seigneuret, M., and Rigaud, J. L. (1990) A systematic study of liposome and proteoliposomes reconstitution involving Bio-Bead mediated TritonX-100 removal. Biochim. Biophys. Acta 1025, 179-190.
25. Bligh, E. G., and Dyer, W. J. (1959) A rapid method of total lipid extraction and purification. Can. J. Med. Sci. 37, 911-917.
26. Kaplan, R. S., and Pedersen, P. L. (1989) Sensitive protein assay in the presence of high levels of lipid. Methods Enzymol. 172, 393-399.
27. Rigaud, J. L., Paternostre, M. T., and Bluzat, A. (1988) Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 2. Incorporation of the light-driven proton pump bacteriorhodopsin. Biochemistry 27, 2677-2688.
28. Vermilion, J. L., and Coon, M. J. (1794) Highly purified detergent solubilized NADPH-cytochrome c P-450 reductase from phenobarbital-induced rat liver microsomes. Biochem. Biophys. Res. Commun. 60, 1315-1322.
29. Vehring, S., Pakkiri, L., Schroer, A., Baerens, N. A., Herrmann, A., Menon, A. K., and Pomorski, T. (2007) Flip-flop of fluorescently labelled phospholipids in proteoliposomes reconstituted with Saccharomyces cereviceae microsomal proteins. Eukaryotic Cell 6, 1625-1634.
30. Nagaoka, T., Hachimori, A., Takeda, A., and Samejima, T. (1977) DTNB modification of SH groups of isocitrate dehydrogenase from Bacillus stearotherophilus purified by affinity chromatography. J. Biochem. 81, 71-88.
31. Follmer, C., and Carlini, C. R. (2005) Effects of chemical modifications of histidines on the copper induced oligomerization of Jack bean urease (E.C. 3.5.1.5). Arch. Biochem. Biophys. 435, 15-20.
32. Morkin, E., Flink, I. L., and Banerjee, S. K. (1979) Phenyl glyoxal modification of cardiac myosin S-I. Evidence for essential arginine residues at active site. J. Biol. Chem. 254, 12647-12652.
33. Barr, S. C., and Warner, K. (2003) Characterization of a serine protease activity Sarcocystis neurona merozoites. J. Parasitol. 8, 385-388.