Catalytically important amino-acid residues of abalone alginate lyase HdAly assessed by site-directed mutagenesis

Enzyme and Microbial Technology

Volumn 43, Issue 6, 2008, Pages 396-402

  Yamamoto, Sayo ^a   Sahara, Takehiko ^b   Sato, Daisuke ^b   Kawasaki, Kosei ^b   Ohgiya, Satoru ^a,b   Inoue, Akira ^a   Ojima, Takao ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Abalone; Alginate lyase; Cold inducible expression in yeast; Haliotis discus hannai; Recombinant enzyme; Site directed mutagenesis

Indexed keywords

ALGAE; ALGINATE; AMINATION; ENZYMES; FOOD ADDITIVES; POLYSACCHARIDES; YEAST;

ABALONE; ACID RESIDUES; ALGINATE LYASE; BROWN ALGAE; CATALYTIC ACTIVITIES; CDNA SEQUENCE; COLD-INDUCIBLE EXPRESSION IN YEAST; EXPRESSION VECTORS; HALIOTIS DISCUS HANNAI; LAMINARIA JAPONICA; OPTIMAL TEMPERATURES; RECOMBINANT ENZYME; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENTS; SITE-DIRECTED MUTAGENESIS; SPECIFIC ACTIVITY; WILD-TYPE; YEAST EXPRESSION;

AMINES;

ALGINATE LYASE HDALY; AMINO ACID; ARGININE; LYASE; UNCLASSIFIED DRUG;

ABALONE; AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME INACTIVATION; LAMINARIA; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

HALIOTIDAE; HALIOTIS DISCUS HANNAI; LAMINARIA JAPONICA; PHAEOPHYCEAE; SACCHAROMYCES CEREVISIAE;

EID: 52949106859     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2008.06.006     Document Type: Article

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